The paper highlights the peculiarities of histological changes in different subdivisions of the intralobular duct of the submandibular gland in rats in case of experimental hyperglycemia. Materials and methods. The study included 40 male Wistar rats weighing 230 to 250g. Experimental hyperglycemia was induced by a single intraperitoneal administration of streptozotocin. Biochemical and morphological investigations were conducted; the morphometric analysis was carried out. Results. Since the 28th day of the experiment, on the background of dynamic increase in the levels of glucose and glycated hemoglobin in the blood, there was observed the development of dystrophic changes in epithelial cells of the granular and striated ducts being accompanied by a gradual decrease in epithelial cell height by 10.28 -29.46% and 10.77 -28.28%, respectively. Morphological changes in the intercalated ducts were detected later -since the 42 nd day of the experiment and the decrease in their epithelial cell height -by 15.60%, was seen on the 70 th day only. Conclusions. Morphological changes in different subdivisions of the intralobular duct are of dystrophic nature and can be histologically detected since the 28 th day of the experiment; they depend on the duration of hyperglycemia and are accompanied by a dynamic decrease in epithelial cell height.
Objective: To demonstrate the capabilities of cluster analysis in receiving scientific innovation results in morphological studies of cells of the bladder urothelium.Materials and methods.10 Wistar rats were used. Histological sections were stained with hematoxylin and eosin; electron microscope studies were conducted; morphometry was performed in ImageJ and statistics – in studio-R using nonparametric methods and multivariate statistics.Results. A brief description of the main stages of cluster analysis shows way to determine the most important features of uroteliocytes and to reveal their heterogeneity, algorithms of Euclidean metrics and methods of clustering were described, the features of the application of the analysis in morphological studies were presented, an example of using these methods in searching for new results was presented, the models of morphological substantiation of clustering results were showed. Conclusion: 1) cluster analysis provides a scientific novelty in studies of transitional epithelium of the bladder; 2) it is used in case of heterogeneity of cellular composition of urothelium that is detected with a help of coefficient of variation; 3) the most significant features of uroteliocytes are their cell area and their nuclei area; 4) new results on the number of clusters were obtained by method of Ward, and new data on their indicators – by k-means; 5) Euclidean metric is the best to use; 6) to assess the adequacy of the results pairwise comparisons between multiple clusters were carried out according to their indicators; 7) results are presented in dimentional projection and they characterize cellular composition of the urothelium as structural system and detect systemic effects.
One of the unresolved issues in experimental neuromorphology is searching for a solution for myelinated nerve fibers clustering on set of morphometric parameters. Therefore, in this article, a new approach for cluster analysis of myelinated fibers is proposed based on their morpho-functional features. The proposed clustering approach was developed in R software environment and uses model-based clustering, which is performed in few steps with increasing number of morphometric parameters on each next step. Applying the proposed clustering solution shown high similarity of identified groups' morphometric parameters with respective physiological types of myelinated A-fibers. This fact, in addition to the algorithm implementation simplicity, facilitates its use on identifying clusters of myelinated fibers that represent different myelinated fibers subpopulation in experimental neuromorphological research with high level of reliability.
The article analyzes the data of literary sources about the histological and ultramicroscopic structure of the endocardium of the ventricles and arteries of various types and caliber normally, as well as under the influence of various endo- and exogenous factors. The data on changes in the structure of the endocardium and major arteries in diabetes mellitus, atherosclerosis, ischemia, hypoxia, infectious endocarditis, poisoning with heavy-density metal salts, and also in hypothyroidism, which has recently been considered as one of the main factors in the development of complications in the cardiovascular system.
The article shows the possibilities of integration of Django web framework and typesetting program LaTeX to automate the creation of methodological guidelines for students by the example of the discipline “Human Anatomy”. The developed system demonstrates the possibilities of separating work on the content and reducing the time for adherence to visual style considering the peculiarities of discipline teaching.
The central organ of hemopoiesis and immune protection is a red bone marrow that contains not only hemopoietic stem cells, but also it forms a unique microenvironment for their proliferation and differentiation. It is a red bone marrow that regulates access to peripheral blood of mature blood corpuscle. Pathological deviations in the blood system are considered to be an increase or decrease in the quantitative composition of individual cells, as well as changes in their ratios relative to each other, or changes in their form.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.