A Gram-stain negative, aerobic, non-motile, short-rod-shaped bacterium, designated KBL-4-9 T , was isolated from the storage liquid in the stems of Populus euphratica trees in Xinjiang, PR China. Strain KBL-4-9 T grew at 4-45 8C (optimum 37 8C), 1-3 % (w/v) NaCl (optimum 1 %, w/v) and pH 5.5-9.5 (optimum pH 7.5). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9 T belonged to the genus Pseudomonas and showed the highest 16S rRNA gene sequence similarity of 97.36 % to Pseudomonas pelagia CL-AP6 T , followed by Pseudomonas xinjiangensis S3-3 T (97.16 %), Pseudomonas sabulinigri J64 T (97.15 %) and Pseudomonas xiamenensis C10-2 T (96.47 %). Analysis of strain KBL-4-9 T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the phylogenetic assignment of the isolates. The DNA G+C content was 61.6 mol% (SD52.19). DNA-DNA hybridization with P. pelagia CL-AP6 T , P. xinjiangensis S3-3 T and P. sabulinigri J64 T revealed 49.3 % (SD53.04), 41.2 % and 52.5 % (SD54.45) relatedness, respectively. The major cellular fatty acids of strain KBL-4-9 T were C 16 : 0 , C 12 : 0 , summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) and C 17 : 0 cyclo. The major isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain KBL-4-9 T is considered to represents a novel species of the genus Pseudomonas, for which the name Pseudomonas populi sp. nov. is proposed. The type strain is

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A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated MC28, was isolated from storage liquid collected from the stems of Populus euphratica in the Xinjiang province of China. The growth range of NaCl concentration was 0.5-6.0 % (w/v), with an optimum at 3.0 % (w/v), the temperature range for growth was 10-45 °C, with an optimum at 40 °C, and the pH range for growth was 6.0-9.0, with an optimum around pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MC28 formed a distinct lineage in the clade of genus Halomonas and is closely related to Halomonas desiderata DSM 9502 (96.4 %), Halomonas heilongjiangensis DSM 26881 (96.2 %) and Halomonas urumqiensis JCM 30202 (95.2 %). The average nucleotide identity, average amino acid identity and in silico DNA-DNA hybridization values between strain MC28 and the references strains were 77.2-80.3, 65.8-76.8 and 21.6-25.6 %, respectively. Chemotaxonomic analysis indicated that the main respiratory quinones were Q-9 and Q-8, the predominant cellular fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω9c, C16 : 0 and C17 : 1ω9c, the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside, an aminophospholipid, an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain MC28 is considered to represent a novel species, for which the name Halomonas endophytica sp. nov. is proposed. The type strain is MC28 (=KCTC 52999=MCCC 1K03343).

Discovery of Three 22-Membered Macrolides by Deciphering the Streamlined Genome of Mangrove-Derived Streptomyces sp. HM190.

An endophytic bacterium, MA-69, was isolated from the storage liquid in the stems of Populuseuphratica trees at the ancient Ugan River in Xinjiang, PR China. Strain MA-69 was found to be short rod-shaped, Gram-stain-negative, non-spore-forming, aerobic and motile by means of a monopolar flagellum. According to phylogenetic analysis based on 16S rRNA gene sequences, strain MA-69 was assigned to the genus Pseudomonas with highest 16S rRNA gene sequence similarity of 97.5 % to Pseudomonas azotifigens JCM 12708, followed by Pseudomonas matsuisoli JCM 30078 (97.5 %), Pseudomonas balearica DSM 6083 (97.1 %), Azotobacter salinestris ATCC 49674 (96.1 %) and Pseudomonas indica DSM 14015 (95.9 %). Analysis of strain MA-69 based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the isolate to be distinctly delineated from species of the genus Pseudomonas. The DNA G+C content of strain MA-69 was 64.1 mol%. DNA-DNA hybridization with Pseudomonas azotifigens JCM 12708, Pseudomonas matsuisoli JCM 30078 and Pseudomonas balearica DSM 6083 revealed 62.9, 60.1 and 49.0 % relatedness, respectively. The major fatty acids in strain MA-69 were summed feature 3 (25.7 %), summed feature 8 (24.0 %), C19 : 0cyclo ω8c (19.9 %), C16 : 0 (14.6 %) and C12 : 0 (6.3 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Q-9 was the major quinone in strain MA-69. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain MA-69 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas tarimensis sp. nov. is proposed. The type strain is MA-69 (=CCTCC AB 2013065=KCTC 42447).

Precise classification of bacteria facilitates prediction of their ecological niche. The genus Enterobacter includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of Enterobacter FY-07 (FY-07), a strain that has many plant-growth-promoting traits and produces bacterial cellulose (BC), to the Kosakonia genera. To re-examine the taxonomic position of FY-07, a polyphasic approach including 16S rRNA gene sequence analysis, ATP synthase β subunit (atpD) gene sequence analysis, DNA gyrase (gyrB) gene sequence analysis, initiation translation factor 2 (infB) gene sequence analysis, RNA polymerase β subunit (rpoB) gene sequence analysis, determination of DNA G + C content, average nucleotide identity based on BLAST, in silico DNA–DNA hybridization and analysis of phenotypic features was applied. This polyphasic analysis suggested that Enterobacter sp. FY-07 should be reclassified as Kosakonia oryzendophytica FY-07. In addition, the potential of FY-07 to promote plant growth was also investigated by detecting related traits and the colonization of FY-07 in rice roots.

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