Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth

Abstract

Precise classification of bacteria facilitates prediction of their ecological niche. The genus Enterobacter includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of Enterobacter FY-07 (FY-07), a strain that has many plant-growth-promoting traits and produces bacterial cellulose (BC), to the Kosakonia genera. To re-examine the taxonomic position of FY-07, a polyphasic approach including 16S rRNA gene sequence analysis, ATP synthase β subunit (atpD) gene sequence analysis, DNA gyrase (gyrB) gene sequence analysis, initiation translation factor 2 (infB) gene sequence analysis, RNA polymerase β subunit (rpoB) gene sequence analysis, determination of DNA G + C content, average nucleotide identity based on BLAST, in silico DNA–DNA hybridization and analysis of phenotypic features was applied. This polyphasic analysis suggested that Enterobacter sp. FY-07 should be reclassified as Kosakonia oryzendophytica FY-07. In addition, the potential of FY-07 to promote plant growth was also investigated by detecting related traits and the colonization of FY-07 in rice roots.

Isolation and Characterization of Endophytic Kosakonia radicincitans to Stimulate Wheat Growth in Saline Soil

“…The in vitro test on Jensen's nitrogen free media showed that the new bacterial isolate could grow on Jensen's medium and was acid producer. This agrees with [47] who suggested that Kosakonia sp. was an associative nitrogen-fixing bacteria or endophytic nitrogen-fixing bacteria.…”

“…This agrees with [51] who proved that pH decrease resulting from the release of organic acids could be related to P & K solubilization. These PGP activities may be due to sharing the bulk of genes that involved in plant growthpromotion as indicated with [47] who found that there were many genes related to promoting plant growth in Enterobacter FY-07.…”

Complete genome sequence of Kosakonia pseudosacchari RX.G5M8, a putative methylotroph isolated from Hong Kong soil

“…Genus Kosakonia , designated in 2013 after reassignment of four species of Enterobacter ( 1 ), currently comprises at least nine species ( 2 ). These include K. sacchari , first isolated from Saccharum officinarum ( 3 , 4 ); and, K. pseudosacchari , found as a Zea mays endophyte ( 5 ).…”

Apirhabdus apintestini gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the western honey bee Apis mellifera

“…In 2016, the Enterobacteriaceae faced taxonomic amendments when whole genome phylogeny was used to propose the order Enterobacterales and subdivide it into seven distinct families, namely Budviciaceae, Enterobacteriaceae, Erwiniaceae, Hafniaceae, Morganellaceae, Pectobacteriaceae and Yersiniaceae [1]. Since then, the family Enterobacteriaceae has undergone further taxonomic revision [2][3][4][5] and currently consists of 37 genera according to the List of Prokaryotic Names with Standing in Nomenclature [6]. Despite some exceptions, most members of the family are motile, catalase positive and oxidase negative.…”