Pseudomonas populi sp. nov., an endophytic bacterium isolated from Populus euphratica

Abstract

A Gram-stain negative, aerobic, non-motile, short-rod-shaped bacterium, designated KBL-4-9 T , was isolated from the storage liquid in the stems of Populus euphratica trees in Xinjiang, PR China. Strain KBL-4-9 T grew at 4-45 8C (optimum 37 8C), 1-3 % (w/v) NaCl (optimum 1 %, w/v) and pH 5.5-9.5 (optimum pH 7.5). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KBL-4-9 T belonged to the genus Pseudomonas and showed the highest 16S rRNA gene sequence similarity of 97.36 % to Pseudomonas pelagia CL-AP6 T , followed by Pseudomonas xinjiangensis S3-3 T (97.16 %), Pseudomonas sabulinigri J64 T (97.15 %) and Pseudomonas xiamenensis C10-2 T (96.47 %). Analysis of strain KBL-4-9 T based on the three housekeeping genes, rpoB, rpoD and gyrB, further confirmed the phylogenetic assignment of the isolates. The DNA G+C content was 61.6 mol% (SD52.19). DNA-DNA hybridization with P. pelagia CL-AP6 T , P. xinjiangensis S3-3 T and P. sabulinigri J64 T revealed 49.3 % (SD53.04), 41.2 % and 52.5 % (SD54.45) relatedness, respectively. The major cellular fatty acids of strain KBL-4-9 T were C 16 : 0 , C 12 : 0 , summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) and C 17 : 0 cyclo. The major isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain KBL-4-9 T is considered to represents a novel species of the genus Pseudomonas, for which the name Pseudomonas populi sp. nov. is proposed. The type strain is

Complete Genome Sequence of Pseudomonas psychrotolerans CS51, a Plant Growth-Promoting Bacterium, Under Heavy Metal Stress Conditions

“…The genus Pseudomonas has a wide distribution, and it is very easy to culture under laboratory conditions [15]. Pseudomonas can be found in various environments, such as plants [16], straw [16], soil [17], animals [18], and saline water [19,20]. Currently, 322 valid species names have been reported in the literature (http://www.bacterio.net).…”

Pseudomonas defluvii sp. nov., isolated from hospital sewage

“…net/ pseudomonas. html) [1], which are Gramnegative aerobic bacteria and widely distribute in various niches of nature [2,3]. Pseudomonas has been considered the most varied and complex groups of Gram-negative bacteria [4].…”

Induced Salt Tolerance of Perennial Ryegrass by a Novel Bacterium Strain from the Rhizosphere of a Desert Shrub Haloxylon ammodendron

“…Among the many known PGPR genera, Pseudomonas has received much research attention because it is widely distributed in various environments and is easy to culture under laboratory conditions [ 18 ]. Pseudomonas can survive and prosper in a wide range of environments with many strains isolated from various environments, such as soil [ 19 ], plant [ 20 ], straw [ 21 ], animal [ 22 ] and fresh and saline water [ 23 , 24 ]. By now, 255 species with validly published names have been described ( ).…”