Halomonas endophytica sp. nov., isolated from liquid in the stems of Populus euphratica

Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated MC28, was isolated from storage liquid collected from the stems of Populus euphratica in the Xinjiang province of China. The growth range of NaCl concentration was 0.5-6.0 % (w/v), with an optimum at 3.0 % (w/v), the temperature range for growth was 10-45 °C, with an optimum at 40 °C, and the pH range for growth was 6.0-9.0, with an optimum around pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MC28 formed a distinct lineage in the clade of genus Halomonas and is closely related to Halomonas desiderata DSM 9502 (96.4 %), Halomonas heilongjiangensis DSM 26881 (96.2 %) and Halomonas urumqiensis JCM 30202 (95.2 %). The average nucleotide identity, average amino acid identity and in silico DNA-DNA hybridization values between strain MC28 and the references strains were 77.2-80.3, 65.8-76.8 and 21.6-25.6 %, respectively. Chemotaxonomic analysis indicated that the main respiratory quinones were Q-9 and Q-8, the predominant cellular fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 1ω9c, C16 : 0 and C17 : 1ω9c, the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannoside, an aminophospholipid, an unidentified phospholipid, an unidentified aminolipid and two unidentified lipids. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain MC28 is considered to represent a novel species, for which the name Halomonas endophytica sp. nov. is proposed. The type strain is MC28 (=KCTC 52999=MCCC 1K03343).

Halomonas radicis sp. nov., isolated from Arthrocnemum macrostachyum growing in the Odiel marshes(Spain) and emended descriptions of Halomonas xinjiangensis and Halomonas zincidurans

“…Additionally, it was here got that strain EAR18 T neither hydrolyses aesculin nor gelatine ( Similar results were already described for other species within the genus [9,15,16,18,19,51] with the exception of urease activity and the capability to assimilate potassium gluconate which are so far only described for the isolate EAR18 T . Finally, strain EAR18 T was catalase positive and oxidase negative in line with other species in the genus [53].…”

“…At the time of writing, Halomonas comprises 94 species [6] being the type species Halomonas elongata [5]. Representatives of the genus have been mainly isolated from saline lakes [7,8], fermented seafood [9,10], saline soils/sediments [11,12,13,14,15], and marine environment [16,17], although recently they were also found associated with plant rhizosphere [18] and inhabiting plant stems [19]. Strain EAR18 T was an endophytic bacterium isolated from roots of an Arthrocnemum macrostachyum plant growing in the Odiel marshes (37º 13'N -6º 57'O) (Huelva, Spain) [20].…”

Halomonas salinarum sp. nov., a moderately halophilic bacterium isolated from saline soil in Yingkou, China

“…The catalase activity and oxidase activity were tested by assessing the formation of bubbles when 3% (v/v) H 2 O 2 was added and the oxidation of tetramethyl-p-phenylenediamine, respectively (Kovacs 1956 ). The antibiotic susceptibility was tested after incubating on MA medium at 30 °C and a 5% salt concentration for 2 days, as described by Chen et al ( 2018a , b ). The physiological and biochemical characteristics were examined using GEN III MicroPlates (Biolog), and API 50 CHB, API ZYM, and API 20NE strips (bioMérieux), following the manufacturers’ instructions.…”

Complete genome sequencing and comparison of two nitrogen-metabolizing bacteria isolated from Antarctic deep-sea sediment

“…The genus Halomonas was described as a facultative anaerobe with gram-negative, straight or curved rodlike cells [11,12]. Halomonas is distributed in Lake Pengyanco on the Tibetan Plateau [13], the Bohai Gulf of the Yellow Sea in China [14], the Pentha beach of Odisha in India [15], Urmia Lake in Iran and other places [16]; it grows in Gobi soil [17], salt lake sediment [18], the liquid in the stems of Populus euphratica [19], the rhizosphere sand of a coastal sand dune plant [20] and other environments, with slight or moderate halophily. Among Halomonas sp., Halomonas bluephagenesis is a relatively comprehensively studied species with engineering tools and methods for genetic modification available.…”