Objectives: To evaluate the efficiency and stability of Nano seashell, Sodium Fluoride and Signal Complete 8 in treatment of dentine hypersensitivity.Methods: Forty freshly extracted premolar teeth were collected, disinfected, and sliced into 1.0± 0.1mm thickness. The dentin discs were randomly divided into two main groups of 20 discs each. Group 1(n=20): Dentin discs subjected to treatment not followed by acid challenge. Group 2 (n=20): Dentin discs subjected to treatment followed by acid challenge. Each group was further subdivided into 4 subgroups of 5 discs each according to the treatment used; Subgroup A (Control group): discs received no treatment; Subgroup B: discs treated with Sodium Fluoride paste; Subgroup C: discs treated with Nano Seashell paste; Subgroup D: discs treated with Signal Complete 8 toothpaste. Evaluation of dentinal tubules obliteration was done under scanning electron microscope.Results: There was a significant change in the diameter of tubules and percentage of occlusion for group 1. Group 2 (subjected to acid challenge after treatment) has shown high stability for subgroup B (Sodium Fluoride), followed by subgroup D (Signal Complete 8) then subgroup C (Nano seashell paste). Conclusion:Nano seashell and Sodium Fluoride caused significant increase of occluded dentinal tubules than Signal complete 8 but the mineral deposits formed by Sodium Fluoride showed higher resistance to acidic environment than those formed by Nano seashell.
Purpose:The objective of this in vitro study was to evaluate and compare the remineralizing potential of dentifrices containing nanohydroxyapatite, fluoride, and bioactive glass with and without fluoride on enamel by assessing the enamel surface microhardness the enamel structural and elemental analysis through Energy Dispersive X-ray Analysis (EDX). Methods: Sound extracted third molars were divided into 5 groups. Group A (n=15): Hydroxyapatite toothpaste (Karex) was used to treat the teeth; Group B (n=15): teeth were treated with Hydroxyapatite and Fluoride containing toothpaste (Apacare); Group C (n=15): teeth were treated with ChloroCalcium Phosphosilicate containing toothpaste (Biomin C); Group D (n=15): teeth were treated with FluoroCalcium Phosphosilicate containing toothpaste (Biomin F); negative control group (n=15): teeth not subjected to any treatment. All teeth (experimental and negative control groups). After 2 weeks of the dynamic pH-cycling; 10 teeth of each group were subjected to microhardness assessment, while 5 teeth of each group were subjected to EDX Analysis. Results: After 2 weeks of pH-cycling, all experimental groups (A, B, C, and D) showed a percent increase of enamel surface microhardness. Group D reported the highest percent increase (15.07%) while teeth that were not subjected to any treatment (negative control group) showed a percent decrease (-15.7%). Fluoride and calcium ions recorded a significantly higher percent increase in group D, while a significantly lower value was recorded in the control group. Conclusion: All the experimental toothpaste had the potential to remineralize enamel surface subjected to dynamic pH-cycling, but the incorporation of fluoride with the bioactive glass technology as in Biomin F toothpaste had the maximum effect on the demineralized enamel surface.
Objective: This in-vitro study evaluated the remineralizing effect of nano-pearl and nano-seashell incorporated into fluoride-based pits-and-fissures sealants by assessing the enamel surface microhardness and the enamel elemental analysis. Materials and Methods: 75 extracted third molars were randomly allocated to one of the following 5 groups of fluoride containing sealant: 10% nano-pearl; 15% nano-pearl; 10% nano-seashell; 15% nano-seashell; or plain fluoride containing sealant. After 2 weeks of dynamic pH-cycling using Pepsi as demineralizing solution; 10 molars of each group were subjected to microhardness assessment, while 5 molars of each group were subjected to Energy Dispersive X-ray Analysis. Results: Teeth treated with 15% nano-pearl reported the highest percentage increase (20.5%) while teeth treated with plain fluoride containing sealant showed a percentage decrease (-11.62 %). The XRD pattern results confirmed the presence of aragonite and calcite polymorphs of CaCO3 in nano-pearl powder and the presence of aragonite in nano-seashell powder. Calcium ions recorded a significantly higher value in the groups treated with 10% and 15% nano-pearl incorporated pits and fissure sealant. Conclusion:The incorporation of 10 % and 15 % nano-pearl and nano-seashell particles into fluoride-based pits and fissure sealant significantly improved the enamel surface microhardness as well as the enamel mineral content.
Objective: The present study was conducted to assess the impact of Moringa Oleifera extracts and ascorbic acid solution on microshear bond strength of composite resin to bleached Enamel. Methods: Twenty-five lower bovine incisors were randomly divided into five groups of 5 teeth each; Group I (n=5) : Where no bleaching was done and no antioxidants were used , Group II (n=5): Where bleaching was done, but no antioxidants were used, Group III (n=5) (Positive control group): Bleaching followed by treatment with 10 % Ascorbic acid solution for 10 minutes, Group IV (n=5): Bleaching followed by treatment with 80 % ethanol extract of Moringa Oleifera for 10 minutes, Group V (n=5): Bleaching followed by treatment with 80 % methanol extract of Moringa Oleifera for 10 minutes. Resin composite was applied on labial enamel surfaces of all groups. The results obtained were explored for normality using Kolmogorov-Smirnov and Shapiro-Wilk tests and One-way ANOVA followed by Tukey post hic test. Results: The results revealed statistically significant difference found between the five groups were p<0.001 where the highest mean value (17.34 Mpa) was recorded for (Group I; No bleaching) followed by (9.22 Mpa) was recorded for (Group III; Ascorbic Acid treatment after bleaching). The least mean value (4.41 Mpa) wasrecorded for (group II; No antioxidant after bleaching). No statistically significant difference was found between (Group III =Ascorbic Acid treatment after Bleaching) and (Group IV= Ethanol extract of Moringa Oleifera treatment after Bleaching). Conclusion: Using 10 % Ascorbic acid solution could reverse the reduced bond strength after bleaching and Ethanol extract of Moringa Oleifera can be a future candidate to be used as antioxidant solution after bleaching if immediate bonding is to be carried out.
Objectives: This study was conducted to evaluate the effect of incorporating Nanocalcium phosphate (NCP) particles into Biodentine™ on pulpal tissue response as a direct pulp capping material (entire tooth culture model). Materials and methods: -A total of forty freshly human premolar teeth extracted for orthodontic reasons were used. After cavity preparation and mechanical pulp exposure, the teeth were divided into 2 main groups (20 teeth each) according to the capping material used; where group (M1) refers to pulps capped with Biodentine™, and group (M2) refers to pulps capped with Biodentine incorporated with NCP. Each group was further subdivided into two subgroups according to the culture period; where subgroup (P1) refers to teeth cultured for 14 days, and subgroup (P2) refers to teeth cultured for 28 days. At the end of each culture period, the teeth were histologically examined to assess the dentine bridge thickness of each group after 14 and 28 days. Moreover, elemental analysis of Calcium and Phosphorous ions in the dentine bridge was performed using SEM-EDX. Results: Direct pulp capping with Biodentine incorporated with NCP showed thicker dentine bridge when compared to Biodentine™ alone. There is a positive correlation between the weight % of Calcium and Phosphorous ions in the dentine bridge below both capping materials and the dentine bridge thickness. Conclusion: Biodentine incorporated with NCP could be a good candidate for pulp capping in the future.
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