Assessment the Impact of Moringa Oleifera Extracts and Ascorbic Acid Solution as Antioxidants on Microshear Bond Strength of Resin Composite to Bleached Bovine Enamel (In Vitro Study)

Abstract

Objective: The present study was conducted to assess the impact of Moringa Oleifera extracts and ascorbic acid solution on microshear bond strength of composite resin to bleached Enamel. Methods: Twenty-five lower bovine incisors were randomly divided into five groups of 5 teeth each; Group I (n=5) : Where no bleaching was done and no antioxidants were used , Group II (n=5): Where bleaching was done, but no antioxidants were used, Group III (n=5) (Positive control group): Bleaching followed by treatment with 10 % Ascorbic acid solution for 10 minutes, Group IV (n=5): Bleaching followed by treatment with 80 % ethanol extract of Moringa Oleifera for 10 minutes, Group V (n=5): Bleaching followed by treatment with 80 % methanol extract of Moringa Oleifera for 10 minutes. Resin composite was applied on labial enamel surfaces of all groups. The results obtained were explored for normality using Kolmogorov-Smirnov and Shapiro-Wilk tests and One-way ANOVA followed by Tukey post hic test. Results: The results revealed statistically significant difference found between the five groups were p<0.001 where the highest mean value (17.34 Mpa) was recorded for (Group I; No bleaching) followed by (9.22 Mpa) was recorded for (Group III; Ascorbic Acid treatment after bleaching). The least mean value (4.41 Mpa) wasrecorded for (group II; No antioxidant after bleaching). No statistically significant difference was found between (Group III =Ascorbic Acid treatment after Bleaching) and (Group IV= Ethanol extract of Moringa Oleifera treatment after Bleaching). Conclusion: Using 10 % Ascorbic acid solution could reverse the reduced bond strength after bleaching and Ethanol extract of Moringa Oleifera can be a future candidate to be used as antioxidant solution after bleaching if immediate bonding is to be carried out.

The antibacterial efficiency of Chitosan photodynamically activated Phycocyanin, and Morinda Oleifera against S.mutans and the bonding strength between composite resin and caries-affected dentin

“…Oxidative stress can compromise the tooth structure and affect the subsequent bond strength between restorative material and CAD. 21 The enhanced bond integrity observed following CAD disinfection with chitosan can be attributed to a study conducted by Ramasetty and colleagues. 22 Their findings suggested that chitosan when used as a cavity disinfectant, enhances the sealing ability and promotes the formation of resin tags between the composite and dentin.…”

Enamel remineralisation prospect of Moringa Oleifera hydrogel, eggshell hydrogel versus sodium fluoride varnish on artificially demineralised primary teeth: in vitro study

“…While teeth showing presence of cracks, fractures, white spots, decalcification, fluorosis, or developmental defects were excluded from the study. Specimens were embedded in self-cure acrylic resin and allowed to set to create blocks [7].…”

“…Teeth were sectioned at cementoenamel junction level. The cut crowns were covered with two layers of acid-resistant varnish leaving only a 4 × 4 mm facial window [7]. The Demineralising solution was composed of 50 mM acetic acid + 2.2 mM Ca(NO3)2.2H 2 O + 2.2 mM KH 2 PO 4 + 0.1 ppm NaF.…”

“…The combined ethanolic extract was evaporated till dryness at 45° C using a rotary evaporator under reduced pressure. The prepared mark was dissolved in water, frozen and lyophilised to obtain a lyophilised dry powder and prepared as a Nanogel hydrogel [7].…”

“…Moreover, occlusion of dentinal tubules by the formation of different-sized mineral particles was observed after the application of Moringa Oleifera [6]. Various animal studies have assessed the safety of Moringa Oleifera extracts and have demonstrated a high degree of safety [7]. Moringa Oleifera extracts have been previously evaluated on the basis of their relative polyphenol, flavonoid contents, and it was found that water extract of leaves exhibits the greatest antibacterial activity [8].…”