Objectives: To evaluate the efficiency and stability of Nano seashell, Sodium Fluoride and Signal Complete 8 in treatment of dentine hypersensitivity.Methods: Forty freshly extracted premolar teeth were collected, disinfected, and sliced into 1.0± 0.1mm thickness. The dentin discs were randomly divided into two main groups of 20 discs each. Group 1(n=20): Dentin discs subjected to treatment not followed by acid challenge. Group 2 (n=20): Dentin discs subjected to treatment followed by acid challenge. Each group was further subdivided into 4 subgroups of 5 discs each according to the treatment used; Subgroup A (Control group): discs received no treatment; Subgroup B: discs treated with Sodium Fluoride paste; Subgroup C: discs treated with Nano Seashell paste; Subgroup D: discs treated with Signal Complete 8 toothpaste. Evaluation of dentinal tubules obliteration was done under scanning electron microscope.Results: There was a significant change in the diameter of tubules and percentage of occlusion for group 1. Group 2 (subjected to acid challenge after treatment) has shown high stability for subgroup B (Sodium Fluoride), followed by subgroup D (Signal Complete 8) then subgroup C (Nano seashell paste).
Conclusion:Nano seashell and Sodium Fluoride caused significant increase of occluded dentinal tubules than Signal complete 8 but the mineral deposits formed by Sodium Fluoride showed higher resistance to acidic environment than those formed by Nano seashell.
Purpose
The purpose of this study was to evaluate and compare the remineralization potential of phosphorylated chitosan nanoparticles (Pchi) and silver diamine fluoride (SDF) compared to sodium fluoride varnish (NaF) on microhardness of artificial carious lesions in a biomimetic minimally invasive approach that is being regarded as the future of preventive dentistry.
Methods
The sample size included 40 intact extracted maxillary anterior human teeth. Baseline microhardness was recorded using Vickers hardness test and energy-dispersive X-ray spectroscopy (EDX). Artificial caries-like lesions were created on the exposed enamel by suspending all teeth in demineralizing solution for 10 days in a temperature of 37 °C and then the hardness and EDX were remeasured. Samples were then divided into four main groups: Group A (positive control group) n = 10, treated with NaF, Group B n = 10, treated with SDF, Group C n = 10, treated with Pchi and Group D (negative control group) n = 10 that received no treatment. After treatment, samples were incubated in artificial saliva solution at 37 °C in for 10 days and then reassessed. Data were then recorded, tabulated, and statistically analyzed using Kruskal–Wallis test and Wilcoxon signed test. Scanning electron microscope (SEM) was used to analyze the morphological changes of enamel surface after treatment.
Results
Groups B and C showed the highest calcium (Ca) and phosphate (P) content as well as hardness values, while group B had the highest percentage of fluoride. SEM revealed a smooth layer of mineral formed on the surface of enamel for both groups.
Conclusion
Pchi and SDF showed the highest increase in enamel microhardness and remineralization potential.
Clinical relevance
The minimally invasive approach for remineralization could be enhanced using SDF and Pchi.
Background: Endodontically treated teeth are subjected to discoloration which must be treated for aesthetic purposes without weakening of tooth structure.Methods: Intra-coronal bleaching using different bleaching agents was tested for affecting the fracture resistance of teeth in three different groups, group 1(n=17) treated with sodium perborate, group 2 (n=17) treated with spirulina extract using photodynamic theory, and group 3 (n=17) control group without bleaching. Fracture resistance of single-rooted extracted premolar teeth was tested using a universal testing machine under a compressive load of 135 degrees on the palatal aspect of the tooth until fracture.Results: Statistical analysis revealed no significant difference between bleaching protocols on fracture resistance of endodontically treated teeth, but they were significantly lower than the nonbleached control group.Conclusions: Bleaching of endodontically treated may compromise the fracture toughness and structural integrity of dentine after root canal treatment with both the conventional bleaching protocol and the photodynamic theory of bleaching.
Antimicrobial photodynamic therapy (aPDT) can be adopted as a modality for bacterial decontamination before cavity restoration to decrease the risk of secondary caries development. Trials to eliminate the microbial load using cavity disinfectants with no adverse effect on the bond strength of adhesive restoration geared toward the long-term success. C-phycocyanin is a safe photosensitizer to be used in disinfecting cavities before restoration especially in esthetic zone as it does not stain the tooth or the restoration as does the toluidine blue. (16) However no studies had evaluated the bond strength of resin to dentin disinfected with C-phycocyanin extracted from spirulina platensis. The teeth (n=48) will be allocated into 2 groups (n=24) according to the type of exposed dentin surface (coronal dentin and radicular dentin). Artificial caries affected dentin of coronal dentin (ACAD) will be induced after pH cycling for 14 days. Dentin surfaces will be disinfected either by using C-phycocyanin as photosensitizer activated using 635-nm diode laser or 2 % chlorohexidine or not disinfected (control group). Then one step self-etch adhesive will be bonded to coronal dentin followed by resin composite packing into the tygon tube applied perpendicular onto the coronal dentin.Self-adhesive resin cement will be bonded to radicular dentin by packing it into the tygon tubes applied perpendicular onto the radicular dentin. Microshear bond strength of self-etch adhesive to coronal dentin; and resin cement to radicular dentin will be tested. One sample from each group will be prepared for scanning electron microscope (SEM) examination.
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