Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweed Turbinaria conoides and their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2–17 nm and 2–19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded against E. coli (17.6 ± 0.42 mm), followed by Salmonella sp., S. liquefaciens, and A. hydrophila. The macrotube dilution method inferred the MIC (20–40 µL mL−1) and MBC (40–60 µL mL−1) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. The Artemia cytotoxicity assay recorded the LC50 value of 88.914 ± 5.04 µL mL−1. Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.
In the present study, an attempt has been made to screen and evaluate the antifouling potentials of the actinobacterial strainStreptomyces fradiaeRMS-MSU isolated from the manakkudy mangroves of Tamilnadu.
The present study was undertaken to investigate the diversity of mangrove-associated bacterial genera at Manakudy estuary, Southwest coast of India. The root and rhizosphere sediments of both mangrove and their associated plants were collected from chosen area. Results inferred that the maximum nutrients, THB density, and diversity indices were recorded in rhizosphere and root sample of Avicennia officinalis. Altogether, 258 bacterial strains were isolated from the chosen mangrove samples and screened for nitrogen fixing and phosphate solubilizing ability. Screened result inferred that, 36.43 % isolates had nitrogen fixing and 29.45 % isolates had phosphate solubilizing ability. Here, the genus Bacillus spp. (21.71 %) was the most dominant genera. The bacterial diversity indices, i.e., univariate analysis showed remarkable variation between the chosen samples; however, maximum diversity indices was registered by rhizosphere and root sample of A. officinalis. The 95 % confidence interval and ellipse showed that samples were well mendacious within AvTD and VarTD. Likewise, the multivariate analysis like similarity percentage was good discriminator from 16.64 to 100 % by Bray-Curtis dissimilarity. The prinicipal component analysis (PCA) showed marked variation between the tested bacterial communities. Cluster analysis and non-metric multi-dimensional scaling (MDS) were grouped by Bray-Curtis similarity index which strongly evidence that the rhizosphere and root samples of A. officinalis were highly diversified in the study area.
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