Bio-prospecting of coral ( Porites lutea ) mucus associated bacteria, Palk Bay reefs, Southeast coast of India

Ahila, Natarajan Kumari; Prakash, S.; Balakrishnan, M.; Ravindran, J.; Prabhu, Nagesh; Ethiraj, Kannapiran

doi:10.1016/j.micpath.2017.09.056

Cited by 10 publications

(5 citation statements)

References 54 publications

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“…Like Pseudomonads, some members of the Moraxellaceae family can break down oil 17 , 53 , and may use that ability to recycle carbon for corals. Bacillaceae bacteria also populate the microbiomes of soft 19 , 54 and stony 54 corals, including tropical scleractinians 36 , 40 , 54 , and may benefit the corals by producing antimicrobial compounds that prevent host infection 54 , 55 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of microbiomes of cold-water corals Primnoa pacifica and Primnoa resedaeformis, with possible link between microbiome composition and host genotype

Goldsmith

Kellogg

Morrison

et al. 2018

Sci Rep

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Cold-water corals provide critical habitats for a multitude of marine species, but are understudied relative to tropical corals. Primnoa pacifica is a cold-water coral prevalent throughout Alaskan waters, while another species in the genus, Primnoa resedaeformis, is widely distributed in the Atlantic Ocean. This study examined the V4-V5 region of the 16S rRNA gene after amplifying and pyrosequencing bacterial DNA from samples of these species. Key differences between the two species’ microbiomes included a robust presence of bacteria belonging to the Chlamydiales order in most of the P. pacifica samples, whereas no more than 2% of any microbial community from P. resedaeformis comprised these bacteria. Microbiomes of P. resedaeformis exhibited higher diversity than those of P. pacifica, and the two species largely clustered separately in a principal coordinate analysis. Comparison of P. resedaeformis microbiomes from samples collected in two submarine canyons revealed a significant difference between locations. This finding mirrored significant genetic differences among the P. resedaeformis from the two canyons based upon population genetic analysis of microsatellite loci. This study presents the first report of microbiomes associated with these two coral species.

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Section: Discussionmentioning

confidence: 99%

Comparison of microbiomes of cold-water corals Primnoa pacifica and Primnoa resedaeformis, with possible link between microbiome composition and host genotype

Goldsmith

Kellogg

Morrison

et al. 2018

Sci Rep

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“…Some examples include Simkania ( Simkaniaceae ), a coral endosymbiont occurring in close association with Endozoicomonas bacteria 71 ; Delftia ( Commamonadaceae ), a key member of coral microbiomes 72 that plays roles in anti-quorum sensing and antibiofilm activity 73 and may help to control pathogenic microbes associated with bleaching 74 ; and Ruegeria ( Rhodobacteraceae ), known for their role in antimicrobial effects against coral pathogens 75 , colonization of early life stages of coral 76 , and degradation of toxic compounds 70 . Other examples include fermentative bacteria such as Limosilactobacillus ( Lactobacillaceae ), formerly classified as Bacillus , isolated from healthy coral mucus 77 , capable of forming stable associations with probiotic bacteria from the genus Lactobacillus 78 . Other fermentative bacteria include Rikenellaceae RC9 gut group, Saccharofermentas , Ruminococcus , Pseudobutyrivibrio , Butyrivibrio , and Christensenellaceae R-7 group, which may play a key role in carbon metabolism and nitrogen cycling within the coral holobiont 63 , 79 , 80 .…”

Section: Discussionmentioning

confidence: 99%

Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

Delgadillo-Ordoñez,

Garcias-Bonet,

Raimundo

et al. 2024

Commun Biol

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Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability of probiotics to restructure the coral microbiome in situ is yet to be determined. As a first step to elucidate this, we inoculated putative probiotic bacteria (pBMCs) on healthy colonies of Pocillopora verrucosa in situ in the Red Sea, three times per week, during 3 months. pBMCs significantly influenced the coral microbiome, while bacteria of the surrounding seawater and sediment remained unchanged. The inoculated genera Halomonas, Pseudoalteromonas, and Bacillus were significantly enriched in probiotic-treated corals. Furthermore, the probiotic treatment also correlated with an increase in other beneficial groups (e.g., Ruegeria and Limosilactobacillus), and a decrease in potential coral pathogens, such as Vibrio. As all corals (treated and non-treated) remained healthy throughout the experiment, we could not track health improvements or protection against stress. Our data indicate that healthy, and therefore stable, coral microbiomes can be restructured in situ, although repeated and continuous inoculations may be required in these cases. Further, our study provides supporting evidence that, at the studied scale, pBMCs have no detectable off-target effects on the surrounding microbiomes of seawater and sediment near inoculated corals.

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“…Although microbes isolated from corals have been reported to produce seco metabolites that could be of technological interest [54][55][56], there is still very l information about the biotechnological enzymatic potential of CAM [55,[57][58][59][60][61]. He screened the bacteria associated with three different coral species from the same and evaluated how the isolated obtained might have different biotechnology pot Investigation of new bioactive compounds is essential not only for industry, but increase even more the economic value of coral reefs, which can eventually contrib enhance policies for their preservation and recovery [62].…”

Section: Methodsmentioning

confidence: 99%

“…Although microbes isolated from corals have been reported to produce secondary metabolites that could be of technological interest [54][55][56], there is still very limited information about the biotechnological enzymatic potential of CAM [55,[57][58][59][60][61]. Here, we screened the bacteria associated with three different coral species from the same region and evaluated how the isolated obtained might have different biotechnology potential.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria

et al. 2021

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Coral-associated microbes are crucial for the biology of their hosts, contributing to nutrient cycling, adaptation, mitigation of toxic compounds, and biological control of pathogens. Natural products from coral-associated micro-organisms (CAM) may possess unique traits. Despite this, the use of CAM for biotechnological purposes has not yet been adequately explored. Here, we investigated the production of commercially important enzymes by 37 strains of bacteria isolated from the coral species Mussismilia braziliensis, Millepora alcicornis, and Porites astreoides. In-vitro enzymatic assays showed that up to 56% of the isolates produced at least one of the seven enzymes screened (lipase, caseinase, keratinase, cellulase, chitinase, amylase, and gelatinase); one strain, identified as Bacillus amyloliquefaciens produced all these enzymes. Additionally, coral species-specific cultured and uncultured microbial communities were identified. The phylum Firmicutes predominated among the isolates, including the genera Exiguobacterium, Bacillus, and Halomonas, among others. Next-generation sequencing and bacteria culturing produced similar but also complementary data, with certain genera detected only by one or the other method. Our results demonstrate the importance of exploring different coral species as sources of specific micro-organisms of biotechnological and industrial interest, at the same time reinforcing the economic and ecological importance of coral reefs as reservoirs of such diversity.

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Bio-prospecting of coral ( Porites lutea ) mucus associated bacteria, Palk Bay reefs, Southeast coast of India

Cited by 10 publications

References 54 publications

Comparison of microbiomes of cold-water corals Primnoa pacifica and Primnoa resedaeformis, with possible link between microbiome composition and host genotype

Comparison of microbiomes of cold-water corals Primnoa pacifica and Primnoa resedaeformis, with possible link between microbiome composition and host genotype

Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria

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