Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

Abstract

Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweed Turbinaria conoides and their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2–17 nm and 2–19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded against E. coli (17.6 ± 0.42 mm), followed by Salmonella sp., S. liquefaciens, and A. hydrophila. The macrotube dilution method inferred the MIC (20–40 µL mL−1) and MBC (40–60 µL mL−1) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. The Artemia cytotoxicity assay recorded the LC50 value of 88.914 ± 5.04 µL mL−1. Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.

Vitis vinifera Assisted Silver Nanoparticles with Antibacterial and Antiproliferative Activity against Ehrlich Ascites Carcinoma Cells

“…In the present study, LC 50 value of the synthesized AgNPs was calculated to be 163 g/mL. In accordance with the present study, Vijayan et al [37] reported 50% mortality of brine shrimp nauplii at 88.91 g/mL concentration of AgNPs synthesized from seaweed Turbinaria conoides, which was a more toxic compound compared to our synthesized AgNPs. Therefore, the biosynthesized V. vinifera mediated AgNPs can definitely be used as an ecofriendly antibacterial and anticancer agent in food and pharmaceutical industries.…”

A Review of Current Research into the Biogenic Synthesis of Metal and Metal Oxide Nanoparticles via Marine Algae and Seagrasses

“…On another front, Ramkumar Vijayan et al have used an aqueous solution containing an extract taken from Turbinaria conoides to biosynthesize both Ag and Au nanoparticles. Their study also investigated the antibiological film formation activity of the nanoparticles against marine biofilm forming bacteria [115]. Also, studies into antimicrobial agents have found that AgCl nanoparticles biosynthesized using an aqueous extract containing Sargassum plagiophyllum were effective agents against bacterial pathogens such as Escherichia coli [116].…”

Nanoparticles and Their Toxicology Studies: A Green Chemistry Approach

“…Their study also investigated the anti biological film formation activity of the nanoparticles against marine biofilm forming bacteria. Also, studies into antimicrobial agents have found that AgCl nanoparticles biosynthesized using an aqueous extract containing Sargassumplagiophyllum were effective agents against bacterial pathogens such as Escherichia coli [41].…”