Introduction: The aim of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) in Algerian hospitals and to characterize the molecular types of carbapenemases found.
Methodology: During a four years study lasting between 2012 and 2015, 81 strains of Enterobacteriaceae with reduced susceptibility to carbapenems were collected from different hospitals. Carbapenemase genes were detected by PCR. Multi locus sequence typing was used to study genetic relationships between carbapenemase- producing Klebsiella pneumoniae isolates.
Results: Among 56 confirmed CPE, blaOXA-48 was detected in 98.21% of isolates. Two isolates co-expressed NDM, and a single one was only an NDM producer. The strains displayed various susceptibility patterns to antibiotics with variable levels of resistance to carbapenems. Multilocus sequence typing (MLST) revealed the presence of multiple sequence types in circulation.
Conclusions: This report highlights the wide distribution of several clones of OXA-48-producing Enterobacteriaceae in Algeria. Urgent action should be taken to avoid epidemic situations.
This study aimed to characterize two third-generation cephalosporins- and
quinolone-resistant Escherichia coli (TGCs- and Q-R-Ec)
isolates recovered from the ovaries of a broiler breeder flock and the internal contents
of hatching eggs produced by the broiler breeder flock. Clonal relatedness was determined
by multilocus sequence typing (MLST). The isolates displayed the same multidrug resistance
profile, with resistance to ampicillin, ticarcillin, piperacillin, cefazollin,
cephalothin, cefotaxime, nalidixic acid, tetracycline and sulfonamides. Double disk
synergy test demonstrated that the two isolates presented an ESBL phenotype. PCR and
sequencing results showed that both the isolates harbored the
blaCTX-M-1 and qnrS1 genes. MLST revealed a
novel allele combination, designated as ST461, in these isolates. This study would
contribute to the molecular epidemiological understanding of TGCs- and/or
Q-R-Ec.
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