Emergence of plasmid mediated carbapenemase OXA-48 in a Klebsiella pneumoniae strain in Algeria

Aggoune, Nadjet; Tali-Maamar, Hassiba; Assaous, Farida; Benamrouche, Nabila; Naim, M.; Rahal, K

doi:10.1016/j.jgar.2014.06.001

Cited by 17 publications

(6 citation statements)

References 11 publications

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“…20 Oxacillinase-type OXA-48 was first identified in Enterobacteriaceae in 2010. 19 The OXA-48 enzyme was detected in an E. coli isolate from a patient from a province bordering Tunisia 21 and recently in a K. pneumoniae isolate from patients in Algiers 22 and Constantine 23 ( Fig. 1 and Table 4).…”

Section: Discussionmentioning

confidence: 97%

First Detection of VIM-4-Producing Pseudomonas aeruginosa and OXA-48-Producing Klebsiella pneumoniae in Northeastern (Annaba, Skikda) Algeria

Mellouk

Bakour

Meradji

et al. 2017

Microbial Drug Resistance

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Section: Discussionmentioning

confidence: 97%

First Detection of VIM-4-Producing Pseudomonas aeruginosa and OXA-48-Producing Klebsiella pneumoniae in Northeastern (Annaba, Skikda) Algeria

Mellouk

Bakour

Meradji

et al. 2017

Microbial Drug Resistance

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“… 3 , 4 , 6 , 8 The occurrence of OXA-48-producing K. pneumoniae has also been reported in Senegal, Saudi Arabia, India, Ireland, Switzerland, and Russia. 6 Recently, in Algeria, a few clinical cases of OXA-48-producing K. pneumoniae have been described: in Algiers (one isolated case, in 2014), 16 in Constantine (three isolated cases, in 2015), 1 in Guelma (two isolated cases, in 2014), 17 in Ouargla (one isolated case recovered between 2014 and 2015), 13 in Batna (seven isolated strains collected between 2014 and 2015), 18 and two strains isolated from the collection of 55 isolates collected between 2013 and 2015 from hospitalized patients in the cities of Annaba (one isolate) and Skikda (one isolate). 2 However, it is important to note that the Enterobacteriaceae family- and also non-fermentative bacilli-producing carbapenemase have always been associated with hospitalized patients.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of carbapenem-resistant Gram-negative bacilli clinical isolates in Algeria

Bourafa¹,

Chaalal²,

Bakour³

et al. 2018

IDR

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ObjectivesThe aims of this study were to investigate the occurrence of carbapenem-resistant Gram-negative bacilli (GNB) isolated from inpatients and outpatients in Algeria between July and September 2015, and to screen their resistance mechanisms and genetic relatedness.Materials and methodsA total of 68 non-redundant isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) antibiotic susceptibility testing was performed using disk diffusion and Etest methods. Carbapenemase activity was carried out using modified Carba NP test, EDTA assay, and the modified Hodge test. Molecular characterization of carbapenemases and extended-spectrum β-lactamase (ESBL) genes were detected by standard PCR and sequencing. Genotyping of carbapenem-resistant isolates was performed by multilocus sequence typing (MLST) analysis.ResultsOf the 68 GNB isolates, 13 (19%) showed reduced susceptibility to carbapenems, including, four Klebsiella pneumoniae, one Escherichia coli, six Acinetobacter baumannii, and two Pseudomonas aeruginosa. blaOXA-48 gene was detected in the five Enterobacteriaceae isolates, and blaOXA-23 was identified in all A. baumannii isolates. OprD mutations were revealed in the two P. aeruginosa isolates. A total of 11 out of the 13 carbapenem-resistant GNB were detected in inpatients, and the two remaining strains were isolated from outpatients. Molecular typing showed the presence of four sequence types (STs) among the OXA-48-producing K. pneumoniae isolates: ST101, ST147, ST163, and ST2017. ST533 was identified for the OXA-48 producing E. coli isolate. All of the A. baumannii and P. aeruginosa were assigned to the international clonal lineages ST2 and ST654, respectively.ConclusionThis study reports the first detection of the epidemic multidrug-resistant lineage, K. pneumoniae ST147 coproduced blaOXA-48 and ESBL genes in Algeria and represents the first description of OXA-48-producing E. coli ST533 and K. pneumoniae ST163 and ST2017. In addition, this study describes for the first time the emergence of OXA-48-producing E. coli and K. pneumoniae in the community in Algeria, leading to major problems for managing microbial infections.

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“…68 However, an earlier study in 2011 found blaOXA-48 genes on a 70 kb plasmid in a K. pneumoniae strain ST307. 2 Other resistance genes, such as aac(6¢)-lb-cr, qnrBl, blaSHV, blaTEM, and blaCTX-M, have been found co-expressed with the blaOXA-48 genes in K. pneumoniae (ST11, ST14, ST16, ST17, and ST101) and E. coli (ST23, ST38, and ST101) strains. 1,68 In A. baumannii, OXA-54, OXA-23, OXA-51, OXA-24, OXA-58, and OXA 24/40 were described between 2010 and 2012 6,29 as mediating high levels of resistance to all b-lactams, gentamicin, amikacin, ciprofloxacin, and tobramycin, 3 with MICs between 8 and 128 mg/L.…”

Section: Egyptmentioning

confidence: 99%

The Molecular Epidemiology and Genetic Environment of Carbapenemases Detected in Africa

Sekyere

Govinden

Essack

2016

Microbial Drug Resistance

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Research articles describing carbapenemases and their genetic environments in Gram-negative bacteria were reviewed to determine the molecular epidemiology of carbapenemases in Africa. The emergence of resistance to the carbapenems, the last resort antibiotic for difficult to treat bacterial infections, affords clinicians few therapeutic options, with a resulting increase in morbidities, mortalities, and healthcare costs. However, the molecular epidemiology of carbapenemases throughout Africa is less described. Research articles and conference proceedings describing the genetic environment and molecular epidemiology of carbapenemases in Africa were retrieved from Google Scholar, Scifinder, Pubmed, Web of Science, and Science Direct databases. Predominant carbapenemase genes so far described in Africa include the blaOXA-48 type, blaIMP, blaVIM, and blaNDM in Acinetobacter baumannii, Klebsiella pneumoniae, Enterobacter cloacae, Citrobacter spp., and Escherichia coli carried on various plasmid types and sizes, transposons, and integrons. Class D and class B carbapenemases, mainly prevalent in A. baumannii, K. pneumoniae, E. cloacae, Citrobacter spp., and E. coli were the commonest carbapenemases. Carbapenemases are mainly reported in North and South Africa as under-resourced laboratories, lack of awareness and funding preclude the detection and reporting of carbapenemase-mediated resistance. Consequently, the true molecular epidemiology of carbapenemases and their genetic environment in Africa is still unknown.

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Emergence of plasmid mediated carbapenemase OXA-48 in a Klebsiella pneumoniae strain in Algeria

Cited by 17 publications

References 11 publications

First Detection of VIM-4-Producing Pseudomonas aeruginosa and OXA-48-Producing Klebsiella pneumoniae in Northeastern (Annaba, Skikda) Algeria

First Detection of VIM-4-Producing Pseudomonas aeruginosa and OXA-48-Producing Klebsiella pneumoniae in Northeastern (Annaba, Skikda) Algeria

Molecular characterization of carbapenem-resistant Gram-negative bacilli clinical isolates in Algeria

The Molecular Epidemiology and Genetic Environment of Carbapenemases Detected in Africa

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