First Detection of VIM-4-Producing <i>Pseudomonas aeruginosa</i> and OXA-48-Producing <i>Klebsiella pneumoniae</i> in Northeastern (Annaba, Skikda) Algeria

Mellouk, Fatma Zohra; Bakour, Sofiane; Meradji, Sameh; Al-Bayssari, Charbel; Bentakouk, Mohamed Cherif; Zouyed, Fatiha; Djahoudi, Abdelghani; Boutefnouchet, Nafissa; Rolain, Jean‐Marc

doi:10.1089/mdr.2016.0032

Cited by 32 publications

(28 citation statements)

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“…Metallo-β-lactamase-producing P. aeruginosa has been extensively reported from different countries in the world [8]. However, data on the prevalent carbapenems resistant phenotypes are limited and poorly investigated in Algeria [9][10][11][12][13]. This work is one among the fewest studies conducted on hospitals in Algeria aiming to project the light on the state of the antibioctic resistance, its level and the main emergent mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital

Merradi

Kassah-Laouar

Ayachi

et al. 2019

J Infect Dev Ctries

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Introduction: Pseudomonas aeruginosa is one of the most common nosocomial pathogens, known with a wide resistance to antimicrobials. Carbapenemases producing Pseudomonas aeruginosa is a growing global public health concern as this pathogen is easily transmissible among patients. Metallo-Beta-lactamases is the most important class of these carbapenemases with their broad-spectrum resistance profile. This study was conducted to investigate the prevalence of MBL-producing P. aeruginosa collected in an Algerian hospital. Methodology: All Metallo-β-lactamase (MBL)-producing P. aeruginosa isolates recovered from patients during a 2 years period (2015-2016) were studied using a combination of phenotypic and molecular typing methods (susceptibility testing, molecular characterization of carbapenemase-encoding genes, multi-locus sequence typing and pulsed-field gel electrophoresis). Results: A total of twenty-six MBL producing P. aeruginosa of 188 isolates were investigated. The burns unit ranked in the first position of the majority of identified cases with 73.07%. About 73.07% of total MBL isolates were mainly isolated from pus samples. The studied isolates were subjected to the molecular typing, in which 4 different Dra1-PFGE patterns and 3 sequences type were assigned (ST244, ST381, and ST1076), and all isolates were revealed positive for VIM-4. Conclusions: We report the third description of blaVIM-4 in Algeria indicating the emergence and spread of carbapenemase-encoding genes among P. aeruginosa in the hospital environment.

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Section: Introductionmentioning

confidence: 99%

Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital

Merradi

Kassah-Laouar

Ayachi

et al. 2019

J Infect Dev Ctries

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“…All the strains were positive for phenotypic carbapenemase production as tested by the modified Carba NP (MCNP) test (7). Real-time and standard PCR and sequencing for ␤-lactamase-encoding genes, which were performed using specific primers for the bla CTX-M , bla SHV , bla TEM , bla KPC , bla NDM , bla VIM , bla IMP , and bla OXA-48 genes (8,9), indicated the presence of the bla CTX-M-15 , bla SHV-1 , bla TEM-1D , and bla OXA-48 genes in all isolates. Multilocus sequence typing (MLST) was performed according to the Institute Pasteur scheme (http://bigsdb.web .pasteur.fr/klebsiella/klebsiella.html) and showed that all strains belonged to sequence type 101 (ST101) except for the Kp1 strain, which was assigned to ST985.…”

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confidence: 99%

Outbreak of OXA-48-Producing Klebsiella pneumoniae Involving a Sequence Type 101 Clone in Batna University Hospital, Algeria

Loucif

Kassah-Laouar

et al. 2016

Antimicrob Agents Chemother

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Seven nonredundant ertapenem-resistant Klebsiella pneumoniae isolates were collected between May 2014 and 19 January 2015 in the nephrology and hematology units of Batna University Hospital in Algeria. All strains coproduced the bla OXA-48 , bla CTX-M-15 , bla SHV-1 , and bla TEM-1D genes. Six of these isolates belonged to the pandemic clone sequence type 101 (ST101). The bla OXA-48 gene was located on a conjugative IncL/M-type plasmid. This is the first known outbreak of OXA-48-producing K. pneumoniae isolates involving an ST101 clone in Batna University Hospital.

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“…The modified Hodge test (MHT) and the modified Carba NP (MCNP) test were used to screen for carbapenemase activity (15,16). All strains were screened using real-time PCR for the presence of the following ␤-lactamase-encoding genes: bla KPC , bla NDM , bla VIM , bla IMP , bla OXA-48 , bla CTX-M , bla TEM , and bla SHV (17)(18)(19). Strains positive by real-time PCR were subjected to standard PCR and sequencing (18,20).…”

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confidence: 99%

First Report of German Cockroaches (Blattella germanica) as Reservoirs of CTX-M-15 Extended-Spectrum-β-Lactamase- and OXA-48 Carbapenemase-Producing Enterobacteriaceae in Batna University Hospital, Algeria

Loucif

Gacemi-Kirane

Cherak

et al. 2016

Antimicrob Agents Chemother

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Here we report the isolation of extended-spectrum ␤-lactamase (ESBL)-and carbapenemase-producing Enterobacteriaceae from German cockroaches caught in the burn unit of Batna University Hospital in Algeria. Nine of 12 isolates harbored the bla CTX-M-15 ESBL gene. One Enterobacter cloacae isolate belonging to sequence type 528 coexpressed the bla OXA-48 , bla CTX-M-15 , and bla TEM genes. Our findings indicate that cockroaches may be one of the most dangerous reservoirs for ESBL and carbapenemase producers in hospitals.T he emergence of multidrug-resistant Enterobacteriaceae (MDRE) and their dissemination in hospitals have become a major health concern worldwide (1). The production of ␤-lactamases such as extended-spectrum ␤-lactamase (ESBL) and carbapenemase remains the main mechanism of resistance in Enterobacteriaceae (2). Algeria is now feaures among the Mediterranean countries known to be affected by the spread of CTX-M-15 type ESBL and the emergence of the phantom menace OXA-48 type carbapenemase (3, 4). Indeed, the transmission and dissemination of MDRE in hospitals are usually related to hand contact (5) and poorly sterilized instruments (6). However, cockroaches which colonize these environments may also act as potential mechanical vectors of antibiotic-resistant bacteria (7). Cockroaches are among the most prevalent pests in hospitals, where they are attracted by moisture, food, and suitable temperatures (8, 9). The medical significance of cockroaches has been largely overlooked, but it has been proven that they can harbor a number of pathogenic microorganisms with different levels of antibiotic resistance (10). Blattella germanica is the most abundant cockroach species (11), and the external surfaces and excrement of these cockroaches can contaminate food, human habitats, and hospital equipment; therefore, these insects can present a threat to human health (12).The aim of our study was to screen for broad-spectrum-cephalosporin-and carbapenem-resistant Enterobacteriaceae in B. germanica cockroaches collected from the burn unit of Batna University Hospital in Algeria and then to investigate the molecular support of ESBL and carbapenemase production.In March 2015, 10 cockroaches were randomly captured from different parts of the burn unit, including the kitchen and the guard, treatment, and patient rooms, directly in sterile containers. Samples were immobilized by freezing at 0°C for 10 min (8). Each cockroach was soaked in 5 ml of Tween 80 solution at 0.05% and vortexed vigorously for 2 min. The resulting wash was used as an external-body-homogenate sample. To remove the external body contamination, cockroaches were immersed in bleach for 2 min, in sterile physiological saline for 2 min, and then in 70% ethanol for 5 min (12). Then, each sample was washed with sterile physiological saline. Subsequently, the insect was soaked in a sterile bottle containing 5 ml of Tween 80 solution at 0.05% and then crushed inside using a sterile pestle. The triturate was then vortexed vigorously for 2 min. The resulting s...

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First Detection of VIM-4-Producing Pseudomonas aeruginosa and OXA-48-Producing Klebsiella pneumoniae in Northeastern (Annaba, Skikda) Algeria

Cited by 32 publications

References 40 publications

Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital

Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital

Outbreak of OXA-48-Producing Klebsiella pneumoniae Involving a Sequence Type 101 Clone in Batna University Hospital, Algeria

First Report of German Cockroaches (Blattella germanica) as Reservoirs of CTX-M-15 Extended-Spectrum-β-Lactamase- and OXA-48 Carbapenemase-Producing Enterobacteriaceae in Batna University Hospital, Algeria

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