AbstractBone regeneration after trauma, pathologic and surgical procedures is considered a major medical challenge. Due to limitations in using conventional approaches, cell based regenerative strategies may provide an alternative option to address such issues. In the current study, we sought to determine the osteogenic potential of dental pulp stem cells (DPSCs) isolated from impacted 3rd molars. DPSCs were isolated from human dental pulp tissue (n=6) using explant culture. Growth characteristics of DPSCs were determined using plating efficiency, and the number and time of population doublings. After characterization, DPSCs were induced to differentiate into osteoblasts and were assessed using polymerase chain reactions (PCR) and histological analysis. Results indicated that DPSCs can be isolated from impacted human third molars, and that DPSCs exhibited typical fibroblastic morphology and excellent proliferative potential. In addition, morphological changes, histological analysis and expression of lineage specific genes confirmed osteogenic differentiation of DPSCs. In conclusion, DPSCs isolated from impacted 3rd molars have high proliferative potential and ability to differentiate into osteoblasts.
Aim: To describe the clinic-o-pathological pattern of patients presented with salivary gland tumors at Department of Maxillofacial surgery Mayo hospital Lahore during time period of one year from 1st January 2019 to 31st December 2019. Methods: This was a retrospective cross-sectional study conducted at Department of Oral and Maxillofacial Surgery, Mayo Hospital Lahore. 75 cases of Salivary gland tumors in the head and neck region were identified. Patient’s medical record and pathology reports were retrieved and carefully reviewed by a senior doctor to collect basic demographic data as well as tumor related information. Data entry and analysis was done with the help of SPSS version-26. Chi Square Test/Fisher exact test was applied to see the association between qualitative variables. Results: In this study Mean age of patients in this study was 40.45±13.18. Male patients were predominant as compared to female patients; Male 53.3% & Female 46.7%.The most frequent tumor among patients was Pleomorphic Adenoma (61.3%) followed by Adenoid Cystic Carcinoma (17.3%), Mucoepidermoid Carcinoma (13.3%), Warthin’s Tumor (4%), Squamous cell Carcinoma of Parotid (2.7%) and adenocarcinoma (1.3%) respectively. However no significant association was seen between salivary gland tumors with age and gender of patients (p-value=0.521). Conclusion: Among the benign salivary gland tumors pleomorphic adenoma is the most common tumor whereas among malignant salivary gland tumors adenoid cystic carcinoma is the most frequent occurring tumor. Keywords: Salivary gland Tumors, Maxillofacial Surgery, Pathological Pattern,
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