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AbstractBone regeneration after trauma, pathologic and surgical procedures is considered a major medical challenge. Due to limitations in using conventional approaches, cell based regenerative strategies may provide an alternative option to address such issues. In the current study, we sought to determine the osteogenic potential of dental pulp stem cells (DPSCs) isolated from impacted 3rd molars. DPSCs were isolated from human dental pulp tissue (n=6) using explant culture. Growth characteristics of DPSCs were determined using plating efficiency, and the number and time of population doublings. After characterization, DPSCs were induced to differentiate into osteoblasts and were assessed using polymerase chain reactions (PCR) and histological analysis. Results indicated that DPSCs can be isolated from impacted human third molars, and that DPSCs exhibited typical fibroblastic morphology and excellent proliferative potential. In addition, morphological changes, histological analysis and expression of lineage specific genes confirmed osteogenic differentiation of DPSCs. In conclusion, DPSCs isolated from impacted 3rd molars have high proliferative potential and ability to differentiate into osteoblasts.

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Aim: To describe the clinic-o-pathological pattern of patients presented with salivary gland tumors at Department of Maxillofacial surgery Mayo hospital Lahore during time period of one year from 1st January 2019 to 31st December 2019. Methods: This was a retrospective cross-sectional study conducted at Department of Oral and Maxillofacial Surgery, Mayo Hospital Lahore. 75 cases of Salivary gland tumors in the head and neck region were identified. Patient’s medical record and pathology reports were retrieved and carefully reviewed by a senior doctor to collect basic demographic data as well as tumor related information. Data entry and analysis was done with the help of SPSS version-26. Chi Square Test/Fisher exact test was applied to see the association between qualitative variables. Results: In this study Mean age of patients in this study was 40.45±13.18. Male patients were predominant as compared to female patients; Male 53.3% & Female 46.7%.The most frequent tumor among patients was Pleomorphic Adenoma (61.3%) followed by Adenoid Cystic Carcinoma (17.3%), Mucoepidermoid Carcinoma (13.3%), Warthin’s Tumor (4%), Squamous cell Carcinoma of Parotid (2.7%) and adenocarcinoma (1.3%) respectively. However no significant association was seen between salivary gland tumors with age and gender of patients (p-value=0.521). Conclusion: Among the benign salivary gland tumors pleomorphic adenoma is the most common tumor whereas among malignant salivary gland tumors adenoid cystic carcinoma is the most frequent occurring tumor. Keywords: Salivary gland Tumors, Maxillofacial Surgery, Pathological Pattern,

Aim: To investigate the etiology, gender distribution and presenting complaints associated with zygomatic fractures Method: This cross sectional study was done in Oral and Maxillofacial Surgery Department of King Edward Medical University from April to D3ecember 2019. Patient data was collected from OPD and analysed with SPSS. Results: We analyzed the data of 162 patients with zygomatic bone fracture. Out of this 148 were male and 14 were females. There were bilateral cases, more affecting right side of face and affecting left side of face. The most common etiology was road traffic accident followed by interpersonal violence and occupational injuries. When we consider presenting complaints 87 patients came with difficulty in chewing, 45 with limited mouth opening, 23 with depressed malar eminence and only 7 patients with nerve paraesthesia. Conclusion: Considering the geographic and cultural indices of the evaluated population, it can be concluded that the patients gender and trauma causes significantly affect the prevalence of zygomatic bone fracture Keywords: Zygomatic bone, nerve paraesthesia, limited mouth opening

Objectives: The aim of this study was to find the interest in research among the undergraduate students of medical and dental colleges of Pakistan. Study Design: Cross-sectional study. Setting: Amongst the students of four medical institutes. Period: April to May 2018. Material & Methods: Sample size was 500 of undergraduate MBBS and BDS students from 1st year to final year. Participants were given a questionnaire and responses were analyzed with SPSS software version 21. Results: The mean age of participants was 23.24 +/- 1.85 years. Female students were more (63%) as compared to male students (37%). Seventy percent (70%) students were interested in different research work and more than 85% students were interested to continue their future career in academics. Participants were more interested in practical training as compared to research and theory. Most of the students were interested to carry out research in clinical field as compared to non-clinical field. The knowledge of research methodology was more in senior students. Student’s knowledge about “impact factor of journal” was low. Conclusion: In this research it has been found that a good number of participants were interested in research activities so measures should be taken to facilitate and motivate them by giving some sort of award to the researchers to increase the publications from Pakistan. Easy access to internet facilities must be provided to promote research activities. Efforts should be made to find out reasons why some students consider research less important. It is also need of the day to develop culture of research by senior faculty members of academic institutes.

Background: Oral Lichen Planus (OLP) is a relatively common chronic inflammatory mucocutaneous disorder. WHO considers OLP a premalignant lesion. This makes management of OLP important to avoid its malignant transformation. Corticosteroids are considered as the first-line of treatment. Different other treatment modalities are also in use for OLP. But there is no statistically significant data available for a particular therapy. The recent evidences suggest methotrexate may effectively be used in low dose in the treatment of OLP. Aim: To compare the frequency of complete resolution of mucosal lesions of oral lichen planus with low dose oral methotrexate versus systemic corticosteroids. Methods: It was a randomized control trial conducted at Oral & Maxillofacial Surgery Department, King Edward Medical University/Mayo Hospital Lahore in six months. A sample of 60 patients was divided into two sub groups namely Group -A (methotrexate) and Group - B (corticosteroid) using lottery method. More than 75% resolution of mucosal lesions clinically was considered as complete resolution at the end of 8th week. Results: The mean age of the patients was 44.55±12.38 years. On 8th week, frequency of complete resolution of mucosal lesions was 73.3% in Group A and 60% in Group B with an insignificant difference (p-value<0.05). Conclusion: Methotrexate group A showed more complete resolution of mucosal lesions than corticosteroid group B with insignificant statistical difference. Keywords: Oral Lichen Planus, Mucosal lesions, Malignant transformation, Methotrexate, Corticosteroids

Objective: To inquire the oral cancer awareness among general medical and general dental professionalsand their knowledge of early detection and prevention of oral squamous cell carcinoma. Study design: cross sectional study Place and duration of study: Oral and maxillofacial surgery Department King Edward Medical University from 5th November 2019 to 5th February 2020. Methodology: A questionnaire was given to dental and medical practitioners and their responses were included in the results. This study was done in three months’ period from 5th November 2019 to 5th February 2020 after ethical approval from institutional review board. Results:The questions were given back by 57 general medical practitioners and by 40 general dental practitioners, producing return rates of 38% GMPs and 26.66% GDPs respectively. The questions were answered by 93 GMPs and by 110 GDPs producing rates of 62% GMPs and 73.33% GDPs respectively. Conclusion: Patients suffering from oral pathologies often go to their general medical practitioner and dental practitioners. The number of patientsof squamous cell carcinoma of oral cavity are rising in the Pakistan, hence general medical and general dental practitioners need to play significant role. This study talks mainly about the on the need for improvement in awareness about oral squamous cell carcinoma. Keywords: oral cancer, awareness, risk factors