Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential

Awais, Sadia; Balouch, Samira Shabbir; Riaz, Nabeela; Choudhery, Mahmood S

doi:10.1515/biol-2020-0023

Cited by 13 publications

(3 citation statements)

References 25 publications

(54 reference statements)

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“…As can be seen from the results, the (+), (c), and (c/+) fractions could successfully synthesize a mineralized matrix, chondrocyte-typical proteins as well as neuronal cell markers. These are important characteristics of DPSCs, which are frequently reported in the literature [ 150 , 151 , 152 , 153 , 154 , 155 ]. The minor differences in the respective expression profiles might be relevant when aiming at the regeneration of a specific cell type.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization

Dieterle

Gross

Steinberg

et al. 2022

Cells

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Human dental pulp stem cells (hDPSCs) are promising for oral/craniofacial regeneration, but their purification and characterization is not yet standardized. hDPSCs from three donors were purified by magnetic activated cell sorting (MACS)-assisted STRO-1-positive cell enrichment (+), colony derivation (c), or a combination of both (c/+). Immunophenotype, clonogenicity, stemness marker expression, senescence, and proliferation were analyzed. Multilineage differentiation was assessed by qPCR, immunohistochemistry, and extracellular matrix mineralization. To confirm the credibility of the results, repeated measures analysis and post hoc p-value adjustment were applied. All hDPSC fractions expressed STRO-1 and were similar for several surface markers, while their clonogenicity and expression of CD10/44/105/146, and 166 varied with the purification method. (+) cells proliferated significantly faster than (c/+), while (c) showed the highest increase in metabolic activity. Colony formation was most efficient in (+) cells, which also exhibited the lowest cellular senescence. All hDPSCs produced mineralized extracellular matrix. Regarding osteogenic induction, (c/+) revealed a significant increase in mRNA expression of COL5A1 and COL6A1, while osteogenic marker genes were detected at varying levels. (c/+) were the only population missing BDNF gene transcription increase during neurogenic induction. All hDPSCs were able to differentiate into chondrocytes. In summary, the three hDPSCs populations showed differences in phenotype, stemness, proliferation, and differentiation capacity. The data suggest that STRO-1-positive cell enrichment is the optimal choice for hDPSCs purification to maintain hDPSCs stemness. Furthermore, an (immuno) phenotypic characterization is the minimum requirement for quality control in hDPSCs studies.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization

Dieterle

Gross

Steinberg

et al. 2022

Cells

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“…Usually, HDPSC morphology is fibroblast-like, but some of the cells changed to a cuboidal or polygonal shape in the mechanical vibration groups at 14 d. It was previously reported that DPSCs showed a fibroblastic-elongated morphology at 7 d in differentiation media [ 22 ], and other studies have reported altered polygonal morphology of DPSCs after 21 d in osteogenic differentiation media [ 23 ]. In addition, Demiray et al have shown that a polygonal morphological change can be induced by mechanical vibrations (90 Hz, 0.15 g) of mouse MSCs during osteogenesis [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

The Osteogenic Differentiation of Human Dental Pulp Stem Cells through G0/G1 Arrest and the p-ERK/Runx-2 Pathway by Sonic Vibration

Lee

Choi

et al. 2021

IJMS

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Mechanical/physical stimulations modulate tissue metabolism, and this process involves multiple cellular mechanisms, including the secretion of growth factors and the activation of mechano-physically sensitive kinases. Cells and tissue can be modulated through specific vibration-induced changes in cell activity, which depend on the vibration frequency and occur via differential gene expression. However, there are few reports about the effects of medium-magnitude (1.12 g) sonic vibration on the osteogenic differentiation of human dental pulp stem cells (HDPSCs). In this study, we investigated whether medium-magnitude (1.12 g) sonic vibration with a frequency of 30, 45, or 100 Hz could affect the osteogenic differentiation of HDPSCs. Their cell morphology changed to a cuboidal shape at 45 Hz and 100 Hz, but the cells in the other groups were elongated. FACS analysis showed decreased CD 73, CD 90, and CD 105 expression at 45 Hz and 100 Hz. Additionally, the proportions of cells in the G0/G1 phase in the control, 30 Hz, 45 Hz, and 100 Hz groups after vibration were 60.7%, 65.9%, 68.3%, and 66.7%, respectively. The mRNA levels of osteogenic-specific markers, including osteonectin, osteocalcin, BMP-2, ALP, and Runx-2, increased at 45 and 100 Hz, and the ALP and calcium content was elevated in the vibration groups compared with those in the control. Additionally, the western blotting results showed that p-ERK, BSP, osteoprotegerin, and osteonectin proteins were upregulated at 45 Hz compared with the other groups. The vibration groups showed higher ALP and calcium content than the control. Vibration, especially at 100 Hz, increased the number of calcified nodes relative to the control group, as evidenced by von Kossa staining. Immunohistochemical staining demonstrated that type I and III collagen, osteonectin, and osteopontin were upregulated at 45 Hz and 100 Hz. These results suggest that medium magnitude vibration at 45 Hz induces the G0/G1 arrest of HDPSCs through the p-ERK/Runx-2 pathway and can serve as a potent stimulator of differentiation and extracellular matrix production.

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“…Mesenchymal Stem Cells (MSCs) often used in stem cell-based therapies, are most often obtained from bone marrow (Bone Marrow Derived-MSCs, BM-MSCs) or adipose tissue (Adipose Derived MSCs, ADSCs), although they can also be obtained from the tooth pulp [ 21 , 22 ]. Such therapies often focus on collecting the target tissue from the patient, culture extracted cells in vitro and use for autogenous transplantation, but commercial lines for allogeneic transplantation are also available [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Antlerogenic stem cells extract accelerate chronic wound healing: a preliminary study

Kmiecik¹,

Kulus

Popiel

et al. 2021

BMC Complement Med Ther

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Background Chronic wounds constitute a significant medical and social problem. Chronic wound treatment may be supported by various techniques, such as negative pressure therapy, phototherapy or stem cells therapy, yet most of those supporting therapies need more evidence to be used for standard wound care. Current study covers the use of sonicated Antlerogenic Stem Cells (ASC) extract on chronic wounds. Methods Study was performed on 20 dermatological patients with venous leg ulcers, divided into two groups – treated with and without ASC extract respectively. The area and circumference of the wounds during the follow-up visits were measured on the wound imprint. Dynamics of wound healing was determined and compared between control and study group; statistics includes changes in absolute values (wound area, circumference), as well as relative (percentage of wound decrease, circumference/area ratio) and their change in time. For the purpose of Ki-67 immunohistochemical staining, sections were sampled from the wound edge at distinct check-points during therapy. Results of both groups were compared with Student test or Mann-Whitney test, depending on results distribution. Results Besides Ki-67 expression, all tested wound healing parameters (including relative and absolute wound decrease and changes in circumference/area ratio) were statistically significant more favorable in experimental group. Conclusion ASC extract significantly supported standard chronic wound treatment. Due to small population of study the results should be considered preliminary, yet promising for further research.

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Human Dental Pulp Stem Cells Exhibit Osteogenic Differentiation Potential

Cited by 13 publications

References 25 publications

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization

The Osteogenic Differentiation of Human Dental Pulp Stem Cells through G0/G1 Arrest and the p-ERK/Runx-2 Pathway by Sonic Vibration

Antlerogenic stem cells extract accelerate chronic wound healing: a preliminary study

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