In non-pigmented and plasmid-deprived mutants-isogenic variants of highly virulent Yersinia pestis 231 strain-studied is the mechanism of biofilm formation on biotic surfaces, both in vitro (on the laboratory model of nematode Caenorhabdiitis elegans) and in vivo (inside the alimentary tract of Nosopsyllus laeviceps flea). It is determined that spontaneous loss of ability to form biofilms and generate pigmented colonies in the mutants is probably caused not only by the deletion of the whole chromosome pigmentation fragment, but also by a point(single base) mutation in structural hms operon. It is demonstrated that the absence of pCad, pFra or pPst plasmids does not have an impact on the ability of plasmid-deprived mutants to form biofilm on the cuticle of nematode C. elegans.
The data of antibiotic-resistant strains of Yersinia pestis isolated from clinical sources and animals are shown in the present study. Highlighted are the reasons of their resistance to different drugs. Represented are two PCRs developed for detection of antibiotic resistance genes, their efficiency has been confirmed using collection of antibiotic-resistant strains of Y. pestis.
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