Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of &lt;I&gt;Yersinia pestis&lt;/I&gt; on Biotic Surfaces, &lt;I&gt;in vivo&lt;/I&gt; and &lt;I&gt;in vitro&lt;/I&gt; Conditions

Eroshenko, G. A.; Vidyaeva, N. A.; Куклева, Л. М.; Koshel, Elena I.; Odinokov, G. N.; Shavina, N. Yu.; Knyazeva, T. V.; Mokrousova, T. V.; Krasnov, Ya. M.; Anisimova, L. V.; Novichkova, L. A.; Erokhin, P. S.; Бойко, А. В.; Кутырев, Владимир

doi:10.21055/0370-1069-2012-3-45-49

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…Some authors surmised the involvement of entomoparasitic nematodes in the transmission of the plague agent [ 4 ], as it was observed that biofilms of Yersinia pestis adhere to cuticle receptors of Caenorhabditis elegans [ 147 – 149 ]. In this perspective, nematodes parasitizing fleas in natural foci of plague take on greater importance, as they may provide for the transmission route that does not include a mammal [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Entomoparasitic Nematodes, Potential Control Agents of Flea Populations in Natural Foci of Plague

Koshel

Aleshin

Eroshenko

et al. 2014

BioMed Research International

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Entomoparasitic nematodes are natural control agents for many insect pests, including fleas that transmit Yersinia pestis, a causative agent of plague, in the natural foci of this extremely dangerous zoonosis. We examined the flea samples from the Volga-Ural natural focus of plague for their infestation with nematodes. Among the six flea species feeding on different rodent hosts (Citellus pygmaeus, Microtus socialis, and Allactaga major), the rate of infestation varied from 0 to 21%. The propagation rate of parasitic nematodes in the haemocoel of infected fleas was very high; in some cases, we observed up to 1,000 juveniles per flea specimen. Our study of morphology, life cycle, and rDNA sequences of these parasites revealed that they belong to three distinct species differing in the host specificity. On SSU and LSU rRNA phylogenies, these species representing three genera (Rubzovinema, Psyllotylenchus, and Spilotylenchus), constitute a monophyletic group close to Allantonema and Parasitylenchus, the type genera of the families Allantonematidae and Parasitylenchidae (Nematoda: Tylenchida). We discuss the SSU-ITS1-5.8S-LSU rDNA phylogeny of the Tylenchida with a special emphasis on the suborder Hexatylina.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Entomoparasitic Nematodes, Potential Control Agents of Flea Populations in Natural Foci of Plague

Koshel

Aleshin

Eroshenko

et al. 2014

BioMed Research International

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“…у штаммов основного подвида в отличие от всех неосновных подвидов выявлены мутации -единичные нуклеотидные замены в генах waaA, hmsT, rcsC и phoP, которые могут отвечать за различия у них в регуляции этого свойства. у беспигментных природных штаммов Y. pestis и у мутантов, дефектных по образованию биопленки, идентифицированы новые типы мутаций, такие как делеция единичного нуклеотида (-а) в гене hmsH, вставка четырех нуклеотидов (+ATGA) в гене hnsS, протяженная делеция участка ypo1969-ypo1926 хромосомной области пигментации, включающая ast, hms и прилегающие к ним гены [50,51].…”

Section: изменения генома обеспечившие трансмиссию Y Pestis с помощьюunclassified

Evolution of Yersinia pestis as the Causative Agent of a Vector-Borne Disease Transmitted by Arthropods

Eroshenko,

Kukleva

2024

Problemy Osobo Opasnykh Infektsii

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The review summarizes the data of modern domestic and foreign studies on the mechanisms of evolutionary adaptation of the plague pathogen to transmissive spread by arthropods. The data on the molecular basis of the rapid formation of a highly pathogenic bacterium due to the acquisition of new genetic information; structural and functional changes in the genome, causing the disruption of functionality of some genes that prevent survival in the vector are presented. The stage of the complex life cycle of the pathogen associated with the peculiarities of its stay in the vector’s organism and its tactics of evasion from the action of antibacterial substances formed by the flea is considered. The importance of biofilm formation for effective transmission of the pathogen is discussed. A complex cascade of transcriptional regulators of biofilm in Yersinia pestis is considered, which includes activators and repressors of biofilm formation, as well as regulators of synthesis or modification/transport of exopolysaccharide. The hms-dependent mechanism of biofilm formation in Y. pestis is described in detail, as well as the impact on biofilm formation through the regulation of LPS-related genes and its role in the modification and transport of biofilm exopolysaccharide. The data from our own studies on the variability of genes involved in biofilm formation in the main subspecies of the plague pathogen in comparison with non-main subspecies of the plague pathogen, as well as on the ability of strains of different subspecies to form biofilm not only in the proventriculus of the flea, but also on the cuticle of soil nematodes of the Tylenchida and Rhabditida orders and the genus Panagrolaimus are presented. The latter allows us to assume the possible participation of soil and entomoparasitic nematodes in the removal of Y. pestis biofilms from the soil to the above-ground biocoenosis of the natural plague focus.

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“…белки, детерминируемые генами hms-оперона, являются компонентами наружной (hmsH и hmsF) и внутренней (hmsR и hmsS) мембран клетки Y. pestis [3]. гены hms-оперона отвечают за образование экзополисахарида, биосинтез которого необходим для проявления штаммами Y. pestis следующих признаков: способность образовывать биопленку, формирование «чумного» блока в преджелудке блохи, образование пигментированных колоний при выращивании на среде с добавлением гемина или красителя конго красного [4,5]. продукция этого экзополисахарида регулируется генами дигуанилатциклазы hmsT и фосфодиэстеразы hmsP, расположенными на значительном расстоянии от оперона и действующими как положительный и отрицательный регуляторы соответственно [6].…”

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Variability of pgm‑Region Genes in Yersinia pestis Strains from the Caspian Sandy and Adjacent Plague Foci

Goryunova

Eroshenko

Куклева

et al. 2023

Problemy Osobo Opasnykh Infektsii

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The aim of the study was to compare the nucleotide sequences of pgm‑region genes in Yersinia pestis strains isolated on the territory of the Caspian sandy and adjacent plague foci in 1925–2015. Materials and methods. 65 Y. pestis strains from the Caspian sandy and adjacent plague foci were used in the work. DNA isolation was performed using the PureLink Genomic DNA Mini Kit. Whole genome sequencing was conducted in Ion S5 XL System (Thermo Fischer Scientific). Data processing was carried out using Ion Torrent Suite software package 3.4.2 and NewblerGS Assembler 2.6. To compare the obtained sequences with the NCBI GenBank database, the Blast algorithm was used. The phylogenetic analysis was performed according to the data of whole genome SNP analysis based on 1183 identified SNPs. The search for marker SNPs was performed using the Snippy 4.6 program. The phylogenetic tree was constructed using the Maximum Likelihood algorithm, the GTR nucleotide substitution model. Results and discussion. The nucleotide sequences of pgm‑region genes of 65 Y. pestis strains from the Caspian sandy and adjacent plague foci have been assessed. Single nucleotide substitutions have been identified in Y. pestis strains from the Caspian sandy and Kobystan plain-foothill foci in the hmsR, astB, ybtS, ypo1944, ypo1943, ypo1936 genes, as well as a deletion of 5 bp in the ypo1945 gene, which is characteristic of strains of one of the phylogenetic lines of Y. pestis from the foci of Caucasus and Transcaucasia, isolated in 1968–2001. The data obtained can be used to differentiate Y. pestis strains from the Caspian sandy focus, as well as to establish the directions of microevolution of the plague pathogen in this region and adjacent foci.

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Studies of Biofilm Formation in Non-Pigmented and Plasmid-Deprived Mutants of <I>Yersinia pestis</I> on Biotic Surfaces, <I>in vivo</I> and <I>in vitro</I> Conditions

Cited by 6 publications

References 7 publications

Phylogenetic Analysis of Entomoparasitic Nematodes, Potential Control Agents of Flea Populations in Natural Foci of Plague

Phylogenetic Analysis of Entomoparasitic Nematodes, Potential Control Agents of Flea Populations in Natural Foci of Plague

Evolution of <I>Yersinia pestis</I> as the Causative Agent of a Vector-Borne Disease Transmitted by Arthropods

Variability of <i>pgm</i>‑Region Genes in <i>Yersinia pestis</i> Strains from the Caspian Sandy and Adjacent Plague Foci

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