The constructed avirulent biplasmid Vibrio cholerae strain KM182 pro ducing cholera toxin B subunit and Escherichia coli colonization factor CFA/1 (providing for antitoxic and anti-colonization immunity formation, correspon dently) was demonstrated to protect immunized model laboratory animals from experimental cholera caused by the virulent Vibrio cholerae O139 strain. The optimal doses for immunization and challenging were determined.
Cellular morphologic variations of Yersinia pseudotuberculosis strain 164/84 were assessed depending on the cultivation temperature and the plague agent's fra-operon carriage by these cells. Notably slower population growth of the two isogenic variants of strain 164/84 (fra+ and fra-) was observed at the cultivation temperatures of 4 to 6 °C as compared with those at 26-28 °C and 36-38 °C. Only late growth stages at the temperatures of 36 to 38 °C brought about significant differences between the fra+ and fra-cell variants expressed in the occurrence of relatively bigger cells in the population of the capsule forming Y. pseudotuberculosis variant.
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