Bovine papillomavirus (BPV) is an oncogenic virus related to serious livestock diseases. Oncoproteins encoded by BPV are involved in several steps of cellular transformation and have been reported as presenting clastogenic effects in peripheral lymphocytes and primary culture cells. The aim of this study was to evaluate the clastogenic potential of BPV types 1, 2, and 4 by comet assay. Peripheral blood was collected from 37 bovines, 32 infected with different levels of papillomatosis (12 animals have no affection) and five calves, virus free (negative control). The viral identification showed presence of more than one virus type in 59.375% of the infected animals. Comet assay was performed according to alkaline technique. The Kruskal-Wallis test showed statistical difference between the negative control group and infected animals (P = 0.0015). The Dunn post hoc test showed difference comparing the infected animals with calves. Mann-Whitney U test verified no difference between animals infected with only one viral type and animals presenting more than one viral type. The comet assay is considered an efficient tool for assessment of damage in the host chromatin due to viral action, specifically highlighting viral activity in blood cells.

ABSTRACT. Bovine papillomaviruses (BPV) are the causal agents of benign and malignant lesions; they can cause dramatic economic losses in cattle. Although 10 virus types have been described, three types are most common in tumors, namely BPV-1, -2 and -4. Previous studies have reported BPV in blood cells and the possibility of blood acting as a latent virus site and/or transmission agent of virus dissemination. We studied a Holstein dairy herd in Pernambuco, Brazil, in which several animals showed severe cutaneous papillomatosis, without previous determination of BPV types. Blood samples and short-term lymphocyte cultures were collected from 54 cows. We compared the BPV types detected in peripheral blood to those identified in the respective lymphocyte cultures: BPV-1 was detected in 74% and BPV-2 in 87% of the whole blood samples. Simultaneous virus presence (BPV-1 and BPV-2) was found in 65% of the blood samples. BPV-1 or BPV-2 were detected in the lymphocyte cultures in 93% of the samples, and both in 89%. The detection of viral DNA in whole blood and in lymphocyte cultures is evidence that this virus is carried by lymphocytes.

The bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, which causes significant economic losses to livestock, characterized by the presence of papillomas that regress spontaneously or persist and progress to malignancy. Currently, there are 13 types of BPVs described in the literature as well as 32 putative new types. This study aimed to isolate viral particles of BPV from skin papillomas, using a novel viral isolation method. The virus types were previously identified with new primers designed. 77 cutaneous papilloma samples of 27 animals, Simmental breed, were surgically removed. The DNA was extracted and subjected to PCR using Delta-Epsilon and Xi primers. The bands were purified and sequenced. The sequences were analyzed using software and compared to the GenBank database, by BLAST tool. The viral typing showed a prevalence of BPV-2 in 81.81% of samples. It was also detected the presence of the putative new virus type BR/UEL2 in one sample. Virus isolation was performed by ultracentrifugation in a single density of cesium chloride. The method of virus isolation is less laborious than those previously described, allowing the isolation of complete virus particles of BPV-2.

ABSTRACT. Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn. The blood diagnosis showed the presence of BPV-2 and the analysis of cutaneous papillomas showed the presence of BPV-4, a new First description of BPV-12 and putative type BAPV8 in Brazil putative virus type BAPV8, and BPV-12, revealing for the first time the presence of BPV-12 and the putative type BAPV8 in beef cattle in Brazil. The sequences were deposited in the GenBank. Histopathology revealed acanthosis, hyperkeratosis, and koilocytosis in all samples analyzed. The presence of BAPV8 and BPV-12 in Brazil emphasizes the ubiquitous dissemination of BPVs in the herds of Brazil.

Ten types of bovine papillomavirus (BPV) have been described and there are reports of viral transmission via blood. The presence of viral DNA in lymphocytes was described to be associated with chromosome instability in these cells. This study presents an evaluation of chromosome instability in short-term peripheral lymphocyte cultures from cows presenting skin papillomatosis, compared with asymptomatic infected animals and non-infected healthy bovines. In a total of 2203 cells, 918 (42%) showed at least one chromosome aberration: 42.7 (± 7.8) in animals with papillomatosis (BPV + W), 40.2 (± 11) in asymptomatic animals (BPV-W) and 4 (± 2) in control animals. Significant differences were found between the infected group (with or without symptoms) and the control group (P < 0.0001). The increased frequencies of chromosome aberrations suggest an interaction between the virus and host cell chromatin.

ABSTRACT. Bovine papillomaviruses (BPVs) are recognized as causal agents of benign and malignant tumors in cattle. Thirteen types of BPVs have already been described and classified into 3 distinct genera. Divergences in the nucleotide sequence of the L1 gene are used to identify new viral types through the employment of PCR assays with degenerated primers. In the present study, a method for identifying BPVs based on PCR-RFLP and DNA sequencing allowed the identification of a new putative Deltapapillomavirus, designated JN/3SP (JQ280500.1). The analysis of the L1 gene showed that this Clinical aspects of a new putative BPV strain was most closely related to the BPVs -1, -2, -13 , and OaPV1 (71-73% genetic similarity). In this study, we describe the detection of this new putative Deltapapillomavirus type and verify its phylogenetic position within the genus.

Este estudo, de natureza teórico-empírica, diz respeito à identificação e análise de atributos relevantes no processo de decisão e compra de vinhos para consumidores do Recife. O modelo teórico adotado foi desenvolvido com base na identificação dos atributos do vinho avaliados mais frequentemente em estudos do comportamento do consumidor em diversos países. Consequentemente, com base no trabalho de Goodman et al. (2007), o grau de relevância de cada atributo foi classificado por meio da escala Best-Worst Scaling (BWS). Destarte, a abordagem metodológica adotada foi a quantitativa, cujo método de coleta de dados foi o questionário, adaptado de Forbes (2008) e Groot (2011), administrado em meio a um amostra de 100 consumidores. Os resultados mostraram que, preço, marca, prêmios, teor alcóolico e rótulo frontal atraente foram considerados atributos importantes para os consumidores. No entanto, os atributos decisivos para a decisão e compra eram outros: variedade da uva, país de origem do vinho, harmonização com alimentos e as informações no rótulo traseiro.

Apresentação do caso: Paciente 46 anos, sexo masculino, sem comorbidades prévias, natural do Piauí e procedente de São Paulo-SP, fazia uso de amitriptilina há 4 meses e negava uso de outras medicações ou álcool, procurou o Pronto Socorro por apresentar quadro de colúria, acolia fecal, febre, prurido e icterícia há 2 dias. Ao exame físico, encontrava-se ictérico, orientado no tempo e no espaço, febril (38,3ºC), abdome inocente. Exames laboratoriais evidenciaram hiperbilirrubinemia importante às custas de bilirrubina direta (bilirrubina total de 18,0 mg/dL; bilirrubina direta de 14,3mg/dL); eosinofilia ( 22,8%); aumento discreto de Gamaglutamil Transferase (122 U/L) e Alanino aminotransferase (125 U/L). Ultrassonografia de abdome e colangiorresonância magnética excluiram processos obstrutivos; sorologias, investigação para Doença de Wilson, hemocromatose, deficiência de A1AT e marcadores de autoimunidade negativos. A biópsia hepática apresentou colestase de padrâo parenquimatosa de natureza indeterminada sugerindo hepatite tóxica ou medicamentosa. Suspenso amitriptilina, tendo, evoluído com melhora sintomática e laboratorial, com exames, após 3 meses da suspensão, apresentando redução de níveis de bilirrubina e da eosinofilia. Discussão: A lesão hepática induzida por drogas (LHID) é causada pela ingestão, inalação ou administração parenteral de agentes químicos ou farmacológicos. O fígado é o órgão responsável por captar, metabolizar e excretar diversas drogas/toxinas, o que o torna mais susceptível à toxicidade e lesão. Este quadro pode apresentar-se desde alterações do perfil bioquímico hepático até à perda de função com insuficiência hepática e necessidade de transplante de fígado, sendo responsável por até 50% dos casos de insuficiência hepática fulminante em todo o mundo. O diagnóstico da LHID é de presunção, afastando-se outras possíveis causas de hepatopatias. Esse trabalho tem como objetivo apresentar um caso de lesão grave induzida por amitriptilina em um paciente sem história prévia de hepatopatia. Comentários finais: Mais de 1100 medicamentos são considerados potencialmente hepatotóxicos, dentre eles a amitriptilina que costuma se apresentar com padrão de acometimento hepatocelular e colestático. O tratamento baseia-se na suspensão da droga e suporte clínico. A melhora clínica e laboratorial completa pode ocorrer dentro de 6 meses a 1 ano após a suspensão da droga. ABCDExpress 2017;1(2):961Codigo: 63968 Acesso está disponível em www.revistaabcd.com.br e www.sbad2017.com.br Acesso pelo