Cytogenetic studies in peripheral blood of bovines afflicted by papillomatosis

Melo, Thatiana Corrêa de; Diniz, N.; Campos, Stella Andrade Rodrigues; Ferraz, O. P.; Lindsey, Charles Julian; Rieger, Tania T.; Beçak, Willy; Stocco, Rita de Cássia

doi:10.1111/j.1476-5829.2011.00263.x

Cited by 20 publications

(27 citation statements)

References 25 publications

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“…The comet assay showed a high clastogenic level in PBMCs infected with BPV. This result reinforces the point that DNA breaks result from viral activity, leading to genomic instability and eventual malignancy (Stocco dos Santos et al, 1998;Melo et al, 2011;Araldi et al, 2013a). The clastogenic action of HPV has already been reported and is associated with spontaneous abortions (Calinisan et al, 2002).…”

Section: Discussionsupporting

confidence: 57%

“…The presence of BPV sequences in peripheral blood has already been described in the literature (Stocco dos Santos et al, 1998;Roperto et al, 2008;Diniz et al, 2009;Melo et al, 2011;Araldi et al, 2013b). The peripheral blood does not represent the first site of virus contact, according to the infection pathway commonly described (Florin et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection

et al. 2015

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ABSTRACT. Bovine papillomavirus (BPV) is an oncogenic virus with mucous and epithelial tropism. Possible productive virus infection in other tissues, such as blood, has been hypothesized. In order to investigate this possibility, three samples of skin papillomas and blood were collected from bovines with BPV infection and five samples of peripheral blood and one sample of normal tissue were collected from a calf without BPV infection. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and examined by reverse transcription-polymerase chain reaction, immunofluorescence, in situ hybridization, and electron microscopy. The tissue samples were examined for histopathological and (2015) immunohistochemical features. The skin papillomas showed the presence of DNA sequences of BPV-2, BPV-11, and a putative virus type. The blood samples showed DNA sequences of BPV-1, 2, and 4 simultaneously. Immunohistochemistry showed BPV L1 protein in both epithelium and stroma and BPV E2 protein in koilocytes. In situ hybridization confirmed the presence of BPV DNA in PBMCs and immunofluorescence showed nuclear labeling of E2 and L1 BPV proteins in PBMCs. The transcription analysis revealed transcripts of BPV-1 L1, BPV-2 L2, and BPV-4 E7 in blood and papilloma samples of BPV-infected cattle. The comet assay revealed high levels of host cell DNA damage upon BPV infection. Electron microscopy analysis of PBMCs identified the presence of particles in the cytoplasm that are consistent with papillomavirus in size and shape. The productive infection of PBMCs with BPV has been previously discussed and this study provides evidence indicating that PBMCs are a target of BPV.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection

et al. 2015

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“…The presence of BPV-2 in peripheral blood leads to chromatin changes (Stocco dos Santos et al, 1998;Melo et al, 2011;Araldi et al, 2013), which result in genomic instability, evidenced by chromosomal loss and gain. This event is frequently observed in solid tumors, with it being associated with the oncogenic process (Duensing and Münger, 2002;Toledo, 2011;Córtes-Gutiérrez et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

et al. 2014

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ABSTRACT. Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn. The blood diagnosis showed the presence of BPV-2 and the analysis of cutaneous papillomas showed the presence of BPV-4, a new First description of BPV-12 and putative type BAPV8 in Brazil putative virus type BAPV8, and BPV-12, revealing for the first time the presence of BPV-12 and the putative type BAPV8 in beef cattle in Brazil. The sequences were deposited in the GenBank. Histopathology revealed acanthosis, hyperkeratosis, and koilocytosis in all samples analyzed. The presence of BAPV8 and BPV-12 in Brazil emphasizes the ubiquitous dissemination of BPVs in the herds of Brazil.

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“…Considering specific situations in which clinical signs that suggest malignance could not be observed, BPV infection could cause cytogenetic aberrations, leading to genomic instability and cancer (Stocco dos Santos et al, 1998;Melo et al, 2011). The bovine lifetime is long enough to allow cancer to arise from years of accumulated mutations.…”

Section: Molecular Diagnosis and Histopathological And Immunohistochementioning

confidence: 99%

Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects

et al. 2015

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ABSTRACT. The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. The comet assay (CA) was used to evaluate the clastogenic potential of BPV. The results confirmed the presence of BPV-2, 3, 5, and 9 in infected samples. Histopathological analysis revealed acanthosis, koilocytosis, hypergranulosis, hyperkeratosis, and transformed fibroblasts. E7 and L1 BPV proteins were detected in the epithelium, as well as in the connective tissues, indicating productive infection at different sites. CA 12943 Genetics and histopathological aspects of BPV ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 12942-12954 (2015) results showed that BPV-2, 5, and 9 exhibit the same level of clastogenicity. These findings support the oncogenic action of BPV in establishing a favorable microenvironment for oncogenesis.

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Cytogenetic studies in peripheral blood of bovines afflicted by papillomatosis

Cited by 20 publications

References 25 publications

Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection

Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects

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