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a b s t r a c tEffects of solvent and supercritical carbon dioxide (SC-CO 2 ) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, b-carotene bleaching and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) assays. Extraction with SC-CO 2 gave a higher yield than solvent extraction (3.15 vs. 0.68 mg/100 g dried decanter, corresponding to 42.99 and 33.63 mg of lycopene).No cytotoxicity was found in Chang liver cells supplemented with either extracts (6.25-200 lg/ml). Solvent extract at 25 lg/ml (2.32 lM lycopene) and SC-CO 2 extract at 200 lg/ml (5.09 lM lycopene) had protective effect against hydrogen peroxide-induced cytotoxicity. However, only high concentrations of solvent extract (200 lg/ml; lycopene = 18.65 lM) or lycopene standard (10 lM) protected cells against DNA damage. Supercritical fluid extraction demonstrated a higher yield in lycopene-rich fraction from decanter. These fractions have the potential to be developed as a functional ingredient to prevent oxidative stress and other related diseases.

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