Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods

Markom, Masturah; Hasan, Mohd Hilmi; Daud, Wan Ramli Wan; Singh, Harcharan; Jahim, Jamaliah Md

doi:10.1016/j.seppur.2006.06.003

Cited by 235 publications

(109 citation statements)

References 19 publications

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“…Pode-se verificar na Tabela 1 que os rendimentos globais obtidos pelo método de Soxhlet, exceto utilizando hexano e éter etílico como solvente, diferenciaram-se dos valores de rendimento global obtidos nas extrações a baixa pressão com e sem ultrassom utilizando etanol como solvente, em todas as condições estudadas. Tal comportamento é explicado devido às condições de temperatura, reciclo do solvente e interações solvente/soluto do método de Soxhlet, que contribuem para a maior solubilização dos solutos da matéria-prima (MARKOM et al, 2007;MAZZUTTI et al, 2012). O baixo rendimento obtido para os experimentos com hexano e éter etílico pode ser explicado devido à polaridade do solvente, uma vez que o hexano tem polaridade menor que a do etanol, que por sua vez teve o maior rendimento entre os solventes testados.…”

Section: Resultsunclassified

EXTRAÇÃO DE PIMENTA MALAGUETA (Capsicum frutescens L.) A BAIXA PRESSÃO ASSISTIDA POR ULTRASSOM

Dias¹,

Santos²,

Aguiar³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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Internacional Agroalimentario, Puerto Real, Cádiz, Espanha RESUMO -A técnica de ultrassom baseia-se na formação de ondas ultrassônicas de alta frequência, capazes de provocar cavitação e causar ruptura na parede celular da matriz vegetal. Isto favorece a penetração do solvente, a transferência de massa e, consequentemente, o aumento do rendimento de extrato. Neste contexto, o objetivo principal do presente trabalho foi obter extratos de pimenta malagueta através da utilização do processo de extração a baixa pressão assistido por ultrassom. A matéria prima utilizada foi a pimenta malagueta desidratada e triturada. Para estudar a influência das variáveis do processo na extração, foram realizados experimentos com e sem ultrassom, nas potências e temperaturas variando de 200 a 600 W e 40 a 60 °C, respectivamente. Em todos os ensaios os parâmetros razão entre matéria prima e solvente e tempo de extração (20 minutos) foram mantidos constantes. A partir dos resultados obtidos pôde-se observar um aumento no rendimento global, na presença de ondas ultrassônicas.

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Section: Resultsunclassified

EXTRAÇÃO DE PIMENTA MALAGUETA (Capsicum frutescens L.) A BAIXA PRESSÃO ASSISTIDA POR ULTRASSOM

Dias¹,

Santos²,

Aguiar³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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“…Upon harvest, leaves were sun-dried and finely ground (88% DM) with 200 mesh sieve. Tannin extraction was conducted using Soxhlet method (Markom et al, 2007), then 5g DM of each C. calothyrsus and L. leucocephala leaf powder was put in timble (Whatman 25x100 mm) with 150ml dissolvent which was 70% acetone containing 1% HCL (Chavan et al, 2001;Shahidi and Naczk, 2004). Heating temperature was adjusted to 6-time dissolution in 1h and extraction was conducted for 3h.…”

Section: Methodsmentioning

confidence: 99%

Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility

Rimbawanto

Yusiati

Baliarti

et al. 2015

anprod

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Abstract. Two plants as condensed tannin (CT) sources were supplemented to protein trash fish silage (TFS) to observe their effect on in vitro ruminal fermentation product, micobial protein synthesis, and digestibility. CT supplementation on protein trash fish silage was on control proportion (0), under optimum level (2.0%), at optimum level (4.0%), and above optimum level (6.0%) of g TFS); of protein precipitation of bovine serum albumin (BSA) with CT from L. leucocephala; and under optimum level (1.5%), at optimum level (3.0%) and above optimum level (4.5% of g TFS), BSA protein precipitation with CT from C. calothyrsus. The effect on degradation in ruminal fluid and ruminal fluid followed by incubation in HCl-pepsin was evaluated using a modified two-step in vitro method. The CT level of L. leucocephala and C. calothyrsus was 4 and 6%, respectively with protein precipitation BSA was 26.25 and 31.77 g BSA/g CT, respectively. CT supplementation to trash fish silage, ruminal fermentation product (NH3-N, C2, C3, and total VFAs) and digestibility (DM and OM) decreased and increased total CP digestibility (in HCl-pepsin). The difference was attributed to CT source without affecting ruminal microbial protein synthesis. CT of L. leucocephala was better in increasing total protein digestibility (70%) than that of C. calothyrsus (15%). It indicated that CT of C. calothyrsus was less effective in protected TSF protein degradation in rumen compared to that of L. leucocephala.Key words: condensed tannin, Leucaena, Calliandra leaves, rumen fermentation, trash fish silage Abstrak. Dua tanaman sebagai sumber tanin kondensasi ditambahkan pada protein silase ikan rucah (SIR) untuk mengetahui pengaruhnya terhadap produk fermentasi dan sintesis protein mikrobia rumen, dan kecernaan secara in vitro. Penambahan tanin kondensasi pada protein silase ikan rucah pada proporsi kontrol (0), di bawah level optimum (2,0%), level optimum (4,0%), dan di atas level optimum (6,0% of g SIR) presipitasi protein bovine serum albumin (BSA) dengan tanin kondensasi L. leucocephala dan di bawah level optimum (1,5%), level optimum (3,0%), dan di atas level optimum (4,5% of g SIR) presipitasi protein BSA dengan tanin kondensasi C. calothyrsus. Pengaruh degradasi di dalam cairan rumen dan inkubasi dalam HCl-pepsin dievaluasi menggunakan metode in vitro dua tahap. Kadar tanin kondensasi L. leucocephala dan C. calothyrsus berturut-turut 4, 6% dengan presipitasi protein BSA 26.25, 31.77 g BSA/g tanin kondensasi. Penambahan tanin kondensasi pada silase ikan rucah, produk fermentasi rumen (NH3-N, C2, C3, and VFAs total) dan kecernaan (BK and BO) di dalam rumen menurun dan meningkatkan kecernaan PK total (dalam HCl-pepsin/paska rumen). Perbedaan tersebut dipengaruhi oleh sumber tanin kondensasi tanpa mempengaruhi sintesis protein mikrobia di dalam rumen. Penggunaan tanin kondensasi dari L. leucocephala lebih baik dalam meningkatkan kecernaan protein total SIR 70% dibanding C. calothyrsus 15%. Ini menunjukkan bahwa tanin kondensasi dari C. calothyrsus kurang ...

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“…Furthermore, methanol is preferred than water to extract phenolic compounds in Phyllanthus species where major components, which are active hydrolysable tannins can be efficiently extracted using semi polar compounds. 31 A study by Cheah and Radu, (2011) 28 also showed that methanol extract had a better TPC and DPPH activity when compared to water extract. Plant antioxidant activity however, also depends on other non-polyphenolic compound such as vitamins, minerals and carotenoids.…”

Section: Antioxidant Assaymentioning

confidence: 96%

Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Water and Methanol Extracts of Phyllanthus species from Malaysia

Zain¹,

Omar²

2018

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Aims:The effects of 2 types of solvents, water and methanol were investigated to determine the presence of antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) from three Phyllanthus species namely, Phyllanthus urinaria, Phyllanthus niruri andPhyllanthus debilis. Material and Methods: The antioxidant activities were measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. The chemical contents of the Phyllanthus sp. were presented as total phenolic content (TPC) and total flavonoid content (TFC). Statistical analysis: All statistical analysis was conducted using SPSS for Windows, Version 22. All data were presented as mean ± standard deviation. Results: Our result showed that P. urinaria showed higher TPC, followed by P. debilis and P. niruri for both methanol and water extracts. Similarly, P. urinaria showed higher TFC than P. debilis and P. niruri. The antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed EC 50 of samples ranged from 15.8 to 29.3 µg/mL for methanol extract and 33.5 to 73.0 µg/mL for water extract. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay showed EC 50 ranged from 11.2 to 26.0 µg/mL for methanol extract and 13.5 to 37.4 µg/mL for water extract. Conclusion: Methanol extract showed higher TPC, TFC value and lower EC 50 values for antioxidant activities when compared to water extract. In both methanol and water extracts, P. urinaria had higher TPC and TFC value and lower EC 50 for both DPPH and ABTS assay followed by P. debilis and P. niruri. Key words: Phyllanthus, Phenolic, Flavonoid, DPPH, ABTS. Key Messages: This paper compared water and methanol extracts between Phyllanthus species in which there is lack of publication available on the effect of different solvents (water and methanol) on the physicochemical profiles (antioxidant, total phenolic and total flavonoid contents) between the species. Our study adds further data information of Phyllanthus debilis, in which this species was not well-researched. We found out that Phyllanthus urinaria, either water or methanol extract, gave better physicochemical profiles compared with P. debilis and P. niruri. Methanolic extract of P. urinaria showed better physicochemical profiles compared with water extract and its biological activity should be further studied.

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Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods

Cited by 235 publications

References 19 publications

EXTRAÇÃO DE PIMENTA MALAGUETA (Capsicum frutescens L.) A BAIXA PRESSÃO ASSISTIDA POR ULTRASSOM

EXTRAÇÃO DE PIMENTA MALAGUETA (Capsicum frutescens L.) A BAIXA PRESSÃO ASSISTIDA POR ULTRASSOM

Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility

Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Water and Methanol Extracts of Phyllanthus species from Malaysia

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