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Plant beneficial microbes mediate biocontrol of diseases by interfering with pathogens or via strengthening the host. Although phytohormones, including cytokinins, are known to regulate plant development and physiology as well as plant immunity, their production by microorganisms has not been considered as a biocontrol mechanism. Here we identify the ability of Pseudomonas fluorescens G20-18 to efficiently control P. syringae infection in Arabidopsis, allowing maintenance of tissue integrity and ultimately biomass yield. Microbial cytokinin production was identified as a key determinant for this biocontrol effect on the hemibiotrophic bacterial pathogen. While cytokinin-deficient loss-of-function mutants of G20-18 exhibit impaired biocontrol, functional complementation with cytokinin biosynthetic genes restores cytokinin-mediated biocontrol, which is correlated with differential cytokinin levels in planta. Arabidopsis mutant analyses revealed the necessity of functional plant cytokinin perception and salicylic acid-dependent defence signalling for this biocontrol mechanism. These results demonstrate microbial cytokinin production as a novel microbe-based, hormone-mediated concept of biocontrol. This mechanism provides a basis to potentially develop novel, integrated plant protection strategies combining promotion of growth, a favourable physiological status and activation of fine-tuned direct defence and abiotic stress resilience.

In order to study the species composition of endophytes from wheat healthy plants in Buenos Aires Province (Argentina) and to determine their infection frequencies from leaves, stems, glumes and grains, wheat plants were collected from five cultivars at five growth stages from crop emergence to harvest. A total of 1,750 plant segments (leaves, stems, glumes and grains) were processed from the five wheat cultivars at five growth stages, and 722 isolates of endophytic fungi recovered were identified as 30 fungal genera. Alternaria alternata, Cladosporium herbarum, Epicoccum nigrum, Cryptococcus sp., Rhodotorula rubra, Penicillium sp. and Fusarium graminearum were the fungi that showed the highest colonization frequency (CF%) in all the tissues and organs analysed. The number of taxa isolated was greater in the leaves than those in the other organs analysed.

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Regional surveys are being conducted in Argentina to assess the presence of wheat ( Triticum aestivum ) pathogens on grains across the main cropping area. During 2001 and 2002, grain samples with a dark brown or blackish discoloration around the embryo end, known as black point, were observed on several cultivars across the wheat region of Buenos Aires Province.Seed analysis by blotter and agar tests (Neergaard, 1979) showed up to 55% of prevalence (number of samples infected over the total) of Alternaria spp. and a mean natural infection of 37%. Morpho-cultural studies were carried out on potato carrot agar. The isolates were typified as belonging to the A. infectoria species-complex according to their sporulation pattern and the production of long secondary conidiophores (Andersen et al ., 2002).Pathogenicity tests were carried out in Petri dishes following two standard methods: blotter test and agar test using 2% potato dextrose agar, inoculating wheat seeds with a conidial suspension (2 × 10 5 conidia mL -1

Tan spot, caused by Pyrenophora triticirepentis, is a common disease of wheat (Triticum aestivum) responsible for economic losses in some wheat growing areas worldwide. In this study the pathogenic and genetic diversity of 51 P. triticirepentis isolates collected from different ecological regions of Argentina were analyzed. Virulence tests were conducted on 10 selected wheat cultivars: Buck Halcón, Chris, Gabo, Glenlea, Klein Dragón, Klein Sendero, Max, ND 495, ProInta Guazú and ProInta Imperial. Data revealed significant differences between all main factors evaluated and the interactions for 19 of the isolates analyzed. Based on the reaction type of each isolate/cultivar combination, 48 different pathogenic patterns were detected. The molecular analysis using Inter-Simple Sequence Repeats (ISSR) revealed the existence of 36 different haplotypes among 37 isolates of P. tritici-repentis originally selected for this study. These results indicate that P. tritici-repentis on wheat in Argentina is a heterogeneous fungus, implying that screening wheat germoplasm for resistance for tan spot disease requires a wide range of pathogen isolates.

Zero tillage oft en leads to wheat (Triticum aestivum L.) yield losses from diseases caused by necrotrophic foliar pathogens. Th e aim of this work was to evaluate the combined eff ect of tillage, N fertilization, fungicides, and resistant cultivars in reducing foliar disease severity to prevent signifi cant yield losses. A 2-yr study including combinations of (i) conventional and zero tillage; (ii) N fertilization rates 0, 80, or 160 kg ha -1 N; (iii) two fungicide treatments (with and without a fungicide (1 L of metconazole, 9%) at growth stages (GS) 32 and 39; and (iv) three wheat cultivars was conducted in the Rolling Pampas region in Argentina. Th e most common foliar disease in the trial was tan spot [Pyrenophora tritici-repentis (Died.) Drechs.]. Conventional tillage reduced foliar disease severity at GS 23 by 46 and 56% and the area under disease progress curve (AUDPC) by 20 and 14% for each season, respectively compared with zero tillage. Th e cultivar Buck Bigua had signifi cantly lower AUDPC values than the others. Fungicide and N application reduced disease severity at GS 23 by 35 and 34% respectively, on average over both years. Disease was less severe in zero tillage plots which received a fungicide compared to conventional tillage plots that were not treated with fungicide. In 2002 yields were greater in conventional tillage plots with 160 kg ha -1 N and fungicide application than in all other treatments. In 2003 yields were greatest in zero tillage plots with 160 kg ha -1 N and fungicide. Th e results of this study indicate that in spite of the increase of necrotrophic diseases, developing no-till systems in wheat monoculture is possible without signifi cant yield losses if eff ective disease management practices are applied.

Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n=62) and England (n=35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.