ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

Abstract

Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n=62) and England (n=35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.

Pyrenophora tritici-repentis, Causal Agent of Tan Spot: A Review of Intraspecific Genetic Diversity

“…However, Moreno et al, (2008b) observed several ISSR haplotypes for P. tritici-repentis isolates obtained from Argentina, using five of 28 ISSR primers tested. Dinolfo et al, (2010), detected similar results for other necrotrophic fungus, Fusarium poae and Misrha et al, (2004) for F. graminearum. In all these works, the conclusion was the high intraspecific variability, but they did not reveal a clear relationship between variability and the host/geographic origin.…”

“…During the last 35 years the genetic of fungal pathogen population of different crops have been extensively analysed (Bayraktar et al, 2007;Clay, 1995;Dinolfo et al, 2010;Goodwing et al, 1995;Stenglein & Balatti 2006;Stenglein et al, 2010;Mc Dermot & Mc Donald, 1993;Moreno et al, 2008Moreno et al, , 2009Tóth et al, 2004). The genetic variability is frequent among plant pathogen populations and its understanding can aid to diseases management decisions (Brown & Wolfe, 1990;Clay, 1995;Milgroon et al, 1992).…”

“…ISSR does not require a previous knowledge of the sequence and generates specific and reproducible patterns due to the high stringent conditions of annealing (Bornet & Branchard, 2001). Several authors has been using this technique to determine the genetic variability in plant pathogenic fungi (Camacho & Liston, 2001;Dinolfo et al, 2010;Moreno et al, 2009;Stenglein & Balatti, 2006;Statkevi´ciute et al, 2010). Within Ascomycota fungi, Stenglein and Balatti (2006), observed a high genetic variability among isolates of Phaeoisaripsis griseola.…”

Fusarium poae Pathogenicity and Mycotoxin Accumulation on Selected Wheat and Barley Genotypes at a Single Location in Argentina

“…Four monosporic F. poae isolates isolated from bread wheat (TSS1a, TSa1a, MICAT01, and MICAT08) and one isolate obtained from barley grains (HSu1a), all confirmed previously by a polymerase chain reaction (PCR) species-specific assay and with the potential to produce NIV, were used individually in this study (10,11). Fungal inoculum was produced by placing individual agar plugs with mycelium and conidia onto potato dextrose agar (PDA; Britania) in petri dishes (90 by 20 mm) and incubating for 7 days at 25 ± 2°C under 12 h each of light and darkness.…”

High genetic variation among Aschersonia placenta (Clavicipitaceae) isolates from citrus orchards in China

“…Inter-simple sequence repeat (ISSR) markers, which have the advantage over RAPDs by employing longer primers that improve reproducibility (Godwin et al, 1997) and are more rapid and economical compared to using the amplified fragment length polymorphism (AFLP) technique (Bornet and branchard 2001), have been widely used to reveal the genetic diversity of fungi, such as the entomopathogenic fungus beauveria bassiana (Aquino de Muro et al, 2005;Wang et al, 2005), the plant pathogenic fungus Corynespora cassiicola (Nghia et al, 2008), Schistosoma japonicum isolates (Zhao et al, 2009), and Fusarium poae isolates (Dinolfo et al, 2010). These studies demonstrated that ISSR may be used as robust molecular markers for studying the population genetics of entomopathogenic fungi.…”