The number of mosquito populations resistant to insecticides is increasing along with the reemerging of vector-borne diseases. New technologies are under evaluation to complement the strategies used against these mosquitoes. Transgenic mosquitoes are one approach that some countries are considering and they are being evaluated to control the wild population. Although they have achieved success in population suppression of Aedes aegypti (L.) (Diptera: Culicidae), these studies have not demonstrated what the outcomes are when releases are interrupted (ceased). In this study, after demonstrating suppression of Ae. aegypti using transgenic technology, changes in the spatial distribution of the infestation and the abundance of the vector Ae. aegypti were assessed in the post-release period, along with fluctuation of transgenic mosquitoes in two areas of Brazil. In both pilot trials, there was an average suppression of ca. 70% of the wild population due to the release of transgenic males compared to the pre-release period. In Juazeiro (Mandacaru), in the post-release phase, the number of eggs per trap ranged between 0.06 and 14.41 (mean AE SE = 4.44 AE 0.44), and the ovitrap index (OI = number of ovitraps with eggs/total number of ovitraps recovered) ranged from 0.01 to 0.43 (0.13 AE 0.01). In Jacobina (Pedra Branca), during the post-release phase, the number of eggs per trap ranged between 1 and 7.2 (1.72 AE 0.72), and the OI ranged from 1 to 0.83 (0.095 AE 0.032). The mosquito population in Juazeiro (Mandacaru) remained suppressed for 17 weeks after the release interruption, whereas in Jacobina (Pedra Branca) suppression lasted 32 weeks. In Juazeiro, transgenic larvae were detected up to 5 months after the interruption of the over-flooding releases of transgenic males. In Jacobina, they were found up to 2 months after the release interruption. The number of eggs collected increased 4-5 months after the release interruption, which indicated that the Ae. aegypti population had been re-established after the interruption of releases. The results demonstrate that the technique requires a continuous release in the treated areas, and after suppression, the release rate can be decreased and used as a barrier against external migration.

Purpose: Quantify the progressive decrease of the cellular viability of rabbit meniscus preserved for transplants over a 30 day period at different freezing temperatures. Methods: 180 menisci were removed from 45 rabbits. Menisci were frozen from 2 to 30 days at -7.2°Celsius -21.4°Celsius and -73°Celsius. Four menisci from each temperature were thawed every two days and the number of present cells was quantified. RESUMOObjetivo: Quantificar a diminuição progressiva da viabilidade celular de meniscos de coelhos preservados para transplantes durante trinta dias em três temperaturas diferentes de congelamento. Métodos: Retirou-se 180 meniscos de 45 coelhos. Os meniscos foram congelados, de dois até trinta dias, a -7,2°Celsius -21,4°Celsius e -73°Celsius. A cada dois dias, de cada temperatura, foram descongelados quatro meniscos e quantificou-se o número de células presentes. Resultados: No 14º dia de congelamento a -7,2°Celsius, a média de células viáveis foi de 92,38%. Entretanto, a partir do 16º dia observou-se uma redução significante de 12% (p=0,001), comparando-se com a média no 14° dia. A média da viabilidade celular, nas temperaturas -21,4°Celsius e -73ºCelsius, até 16º dia apresentou comportamento estatisticamente semelhante. A partir do 18º dia na temperatura de -21,4°Celsius a redução do número de células foi significante (p<0,001), especialmente do 28°( 54,5%) para o 30° dia (30%) de congelamento. O número de células viáveis a -73°Celsius mostrou uma redução não significante (p=1,000) de 2,3% no número de células viáveis do 28° (40,2%) ao 30° dia (37,9%). Conclusão: A quantidade de células de meniscos viáveis é semelhante até o décimo quarto dia de preservação, em qualquer das três temperaturas testadas. No entanto, à partir deste período até o trigésimo dia a quantidade de células viáveis é menor nas temperaturas mais baixas. Descritores: Congelamento. Meniscos. Transplante. Coelhos.

Dioctophymosis is a worldwide renal parasitosis caused by the Dioctophyma renale nematode, which results in progressive destruction of renal tissue. Aquatics annelids are considered the main intermediate hosts and the literature refers as permanent hosts of dogs, wild mammals and even humans. During procedures for population control of coatis (Nasua nasua) in the Ecological Park of Tietê (PET), was noticed the presence of parasitosis by D. renale. From 68 animals, males and females, young and adults, submitted to exploratory laparotomy, 51 were positive for the presence of worms, 9 were found only in the right kidney. In 10 cases, in addition to right kidney parasitism, worms were also observed in the abdominal cavity. In 24 cases D. renale was found only in the abdominal cavity and in 8 animals the right kidney was reduced to a small rigid structure. The study showed that the preferred site for parasitism of the worm, considered erratic, was the abdominal cavity in 66.66% of the cases.

Introdução. Patologias neurológicas são as doenças do siste­ma nervoso central e periférico que incluem desor­dens do encéfalo, da medula espinhal, dos nervos pe­riféricos e da junção neuromuscular. Os indivíduos acometidos por lesões neurológicas necessitam dos cuidados da reabilitação realizados por uma equipe multidisciplinar. Diante a isto, a fisioterapia faz- se importante na reinserção desses pacientes no contexto social. Objetivo. O presente estudo objetivou descrever o perfil epidemiológico e clínico dos pacientes neurológicos atendidos no ambulatório de reabilitação do Hospital Universitário da Universidade Federal do Vale do São Francisco (HU-UNIVASF). Método. Trata-se de um estudo observacional de natureza descritiva, transversal de caráter retrospectivo, com a coleta de prontuários de pacientes neurológicos entre maio de 2015 a maio de 2018. Os dados foram processados e analisados utilizando o programa Statistical Package for the Social Sciences – SPSS. Resultados. Os dados demonstraram, que do total pesquisado, o gênero masculino foi predominante, na faixa etária entre 34 e 67 anos, em sua maioria trabalhadores rurais, sendo o AVC a condição clínica mais prevalente com maior frequência para o tipo isquêmico. A maioria dos pacientes não apresentou causa definida das patologias e a melhora do quadro clínico foi o principal motivo da alta fisioterapêutica. Conclusão. Este estudo contribuiu para o conhecimento sobre o perfil da região, bem como para despertar o interesse de entidades representativas da sociedade para o fortalecimento de políticas públicas direcionadas para a reabilitação e prevenção de novos casos.

PURPOSE:To investigate the morphological aspects of the healing of traumatic wounds in rats using low-power laser. METHODS:Twenty four non isogenic, young adult male Wistar rats (Rattus norvegicus) weighing between 200 and 300g was used. The animals were randomly distributed into two groups: Control (GC) and Laser (GL), with 12 animals each. After shaving, anesthesia was performed in the dorsal region and then a surgical procedure using a scapel was carried out to make the traumatic wound. GL received five sessions of laser therapy in consecutive days using the following laser parameters: wavelength 660 nm, power 100 mW, dose 10 J/cm 2 . The wounds were evaluated through measurement of the area and depth of the wound (MW) and histological analysis (HA). RESULTS:When comparing the GC with the GL in MW there was a difference in area (p<0.001) and depth (p=0.003) measurement of the wounds in GL. The laser group presented more epithelization than GC (p=0.03). The other histological parameters were similar. CONCLUSION:The healing of wounds in rats was improved with the use of the laser.

Pesq. Vet. Bras. 33(7): 942-948, julho 2013 942 RESUMO.-Glândulas vesiculares são essenciais para a reprodução, pois suas secreções afetam a função espermá-tica. Cobaias (Cavia porcellus) são um excelente modelo experimental para estudo destas glândulas, contudo não existem dados morfológicos e morfométricos durante seu desenvolvimento. Neste estudo a morfologia (projeções das pregas (PP) e altura das células epiteliais (AE) da tú-nica mucosa) e a morfometria (massa (MG), volume (VG), comprimento (CG), largura das porções cranial (LCR), mé-dia (LM) e caudal (LCA)) das glândulas vesiculares foram determinadas em cobaios (N= 25) com uma (S1), três (S3), (Cavia porcellus) are an excellent experimental model for studying vesicular glands, but no morphologic and morphometric data during its development are available. In the present study, the morphology (projections of the folds (PF) and epithelial tissue high (EH) of the mucosa) and the morphometry (mass (GM), volume (GV), length (GC), width of the portions cranial (CRW), medium (MW) and caudal (CAW)) of the vesicular glands were determined at 1, 3, 5, 8, and 11-weeks of age in male guinea pigs (n = 5/age group). In addition, body mass (BM), body length (BL) and height (BH) and organo-somatic index (OSI) were also determined and the simple correlation coefficient (r) was established between the variables. The glands were restricted to the pelvic cavity, and had its interior filled with a semi-solid mass and, after S8, they occupied also the abdominal cavity and the contents were increased significantly. The mucous membrane presented variable folding and simple columnar epithelium. BM, BL and BH increased continuously with age. GM, GV, GC and CAW did not change from 1 to 5-weeks of age, but increased at 8-weeks of age; GM, GV and LCA also increased at 11-weeks of age. OSI and PF increased at 8 and 11-weeks of age and EH at 5, 8 and 11-weeks of age. There was significant (r) between age, body and glandular parameters; OSI and GM at 8 and 11-weeks of age; age and PF, age and EH and between PF and EH. In conclusion, the guinea pigs vesicular glands followed morphology observed in rats and hamsters, but differed in some aspects from others histricomorfs, can be used as experimental model and its morphological and morphometric development can be divided into three phases: 1 to 5-weeks of age, when they are discrete; 6 to 8-weeks of age when they are generally sharp and >8-weeks of age, when there is an intense increase of the secretory capacity.

OBJECTIVES:The purpose is to study the effects of hyperbaric oxygen therapy and autologous platelet concentrates in healing the fibula bone of rabbits after induced fractures.METHODS:A total of 128 male New Zealand albino rabbits, between 6–8 months old, were subjected to a total osteotomy of the proximal portion of the right fibula. After surgery, the animals were divided into four groups (n = 32 each): control group, in which animals were subjected to osteotomy; autologous platelet concentrate group, in which animals were subjected to osteotomy and autologous platelet concentrate applied at the fracture site; hyperbaric oxygen group, in which animals were subjected to osteotomy and 9 consecutive daily hyperbaric oxygen therapy sessions; and autologous platelet concentrate and hyperbaric oxygen group, in which animals were subjected to osteotomy, autologous platelet concentrate applied at the fracture site, and 9 consecutive daily hyperbaric oxygen therapy sessions. Each group was divided into 4 subgroups according to a pre-determined euthanasia time points: 2, 4, 6, and 8 weeks postoperative. After euthanasia at a specific time point, the fibula containing the osseous callus was prepared histologically and stained with hematoxylin and eosin or picrosirius red.RESULTS:Autologous platelet concentrates and hyperbaric oxygen therapy, applied together or separately, increased the rate of bone healing compared with the control group.CONCLUSION:Hyperbaric oxygen therapy and autologous platelet concentrate combined increased the rate of bone healing in this experimental model.

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