Angiogenesis, the formation of new capillaries from pre-existing vessels, is essential for tumor progression. Synthetic derivatives of
anti-cancer compound, noscapine (an opium alkaloid) such as Cl-noscapine, Br-noscapine and Folate-noscapine along with two of the
reference compounds, TNP-470 and paclitaxel were examined for anti-angiogenic activities by using human umbilical vein endothelial
cells (HUVECs). The noscapine derivatives showed anti-angiogenic activity albeit at high concentration compared to the reference
compounds. All the tested compounds inhibited angiogenesis in a dose-dependent manner; the drug concentration causing 50%
inhibition of cell survival was 11.87 μM for Cl-noscapine, 6.9 μM for Br-noscapine and 6.79 μM for folate-noscapine. Besides, all the
noscapine derivatives significantly inhibited cord formation (IC50 for Cl-noscapine is 50.76 μM, for Br-noscapine is 90.08 μM and for
folate-noscapine is 18.44 μM) as well as migration and invasion (IC50 value of Cl-noscapine is 28.01 μM, for Br-noscapine is 19.78 μM
and for folate-noscapine is 10.76 μM) of endothelial cells. Based on these results, we speculated that the inhibitory effects on human
endothelial cell proliferation of noscapine derivatives might be important for anti-angiogenesis.
Objectives:The aim was to investigate the antipyretic, anti-infl ammatory and analgesic activity of ethanolic leaf extract of Moringa oleifera, commonly known as drumstick tree. Materials and Methods: The study is a randomized controlled experimental study. The experiments were carried out dividing the animals in six groups, each containing six animals. Ethanolic extract of Moringa M. oleifera (EMO was administered at 50, 100, 200, 400 mg/kg doses orally to the respective four groups. Control was normal saline (orally at 2 ml/kg body weight). Antipyretic activity was done in albino rats using the Brewer's yeast induced pyrexia model, standard used was paracetamol (100 mg/kg). Anti-infl ammatory action was screened using carrageenan induced paw edema model in albino rats. Analgesic actions was evaluated using acetic acid induced writhing test and Eddy's hot plate test for the peripheral and central analgesic actions respectively using albino mice. Results: The ethanolic leaf extract of M. oleifera showed signifi cant (P < 0.05) antipyretic and anti-infl ammatory activities at 100, 200, 400 mg/kg. The percentage inhibition of paw edema at 3 rd h was 64.77% for aspirin and EMO 400 mg/kg showed 56.81% comparing with the control. Signifi cant (P < 0.01) analgesic activity was exhibited by ethanolic leaf extract of M. oleifera at 100, 200, 400 mg/kg in both the acetic acid induced writhing test and the Eddy's hot plate test in comparison with control. Dose-dependent increase in the percentage inhibition of writhes was noted with EMO 100, 200, 400 mg/kg showing 26%, 51% and 81%, respectively. Conclusion: Thus, our study concludes that EMO leaves has antipyretic, anti-infl ammatory, and both central and peripheral analgesic actions.
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