Aeromonas hydrophila bacteria was isolated from the suspected EUS-affected shing fish, Heteropneustes fossilis (Bloch). The disease investigations were primarily based on clinical signs and subsequently confirmed by the isolation of bacterial pathogen Aeromonas hydrophila from lesion of liver and kidney. The A. hydrophila isolates were identified by a series of morphological, physiological and biochemical tests. The total bacterial load in liver, intestine and kidney were 1.67 × 10 4 to 6.46 × 10 8 CFU/g, 1.71 × 10 3 to 1.18 × 10 9 CFU/g and 1.47 × 10 4 to 3.70 × 10 8 CFU/g respectively.

Objectives : To determine the prevalence, aetiology and susceptibility profile of bacterial agents of wound infection among in- and- out patients.Methods : Wound swabs collected from 150 patients were, cultured and microbial isolates identified using standard methods. Antibiotic susceptibility testing was done on bacterial isolates.Results : Of the 150 swabs 131 (87.4%) were culture positive for bacterial pathogens, while 19 (12.6%) were bacteriologically sterile showing an isolation rate of 87.4%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%). All isolates showed high frequency of resistance to ampicillin, penicillin, cephalothin and tetracycline. The flouroquinolones were the most potent antimicrobial agents against bacterial isolates from both in - and out -patients.Conclusion : Staphylococcus aureus was the most predominant etiologic agent of wound infection among in and out patients. A generally higher resistance pattern was observed among nosocomial bacterial pathogens. Prudent use of antibiotics is recommended.TAJ 2011; 24(2): 136-141

Objective: The present study was done to see the antimicrobial susceptibility pattern of bacterial isolates from wound infection.Methods: Wound swabs collected from 150 patients of wound infection were cultured and microbial isolates identified using standard methods. Antimicrobial susceptibility testing was done on bacterial isolates by Modified Kirby Bauer method.Results: Of the 150 swabs 131 (87.4%) were culture positive for bacterial pathogens, while 19 (12.6%) were bacteriologically sterile showing an isolation rate of 87.4%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%).In case of gram positive bacteria- rate of isolates resistant to ampicillin was 94%, followed by penicillin G, 86.8%, Tetracycline, 51.5%. In case of gram negative bacteria- rate of isolates resistant to ampicillin was 96%, followed by cephalothin, 92.4%, Tetracycline, 74%.Conclusion: In antimicrobial susceptibility testing ampicillin, penicillin, cephalothin and tetracycline were the least effective. Gentamicin, norfloxacin, ciprofloxacin, vancomycin and amikacin were the most effective antibiotics.TAJ 2013; 26: 61-66

Objective: The present study was done on Chromogenic agar media to identify uropathogens more efficiently by its characteristic colony colour for each of the organism.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto Chromogenic agar media, Blood agar and MacConkey agar media.Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both Chromogenic agar media and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on Chromogenic agar media when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on Chromogenic agar media in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on Chromogenic agar media, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: Chromogenic agar media has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2012; 25: 64-71

We report a case of middle aged man who presented with stroke with right sided weakness. Later on, routine investigations reveal erythrocytosis on complete blood count and left renal mass on USG of abdomen. Further investigations correlate the above scenario with renal cell carcinoma.TAJ 2009; 22(1): 248-250

Objective: The present study was done to compare the performance of chromogenic agar medium and conventional culture media for the isolation and presumptive identification of uropathogen.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh during January to June, 2008. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto 2 conventional media (Blood agar and MacConkey agar) and chromogenic agar medium (HiCrome UTI agar medium). Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both HiCrome UTI agar and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on HiCrome UTI agar when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on HiCrome UTI agar medium in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on HiCrome UTI agar, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: HiCrome UTI agar medium has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2011; 24(2): 128-135

Then Present study was done to assess the distribution of bacterial pathogens with their pattern of antibiotic susceptibility in an urban referral hospital in RMCH. A total of 393 bacteria strains were isolated from various specimens over a 10-months period. The majority of the organisms were Escherichia coli (33.33%) followed by Klebsiella species (27.48%), staphylococcus aureus (17.05%), Acinetobcter species (8.14%), Pseudomonas species (7.12%), and others. The third-generation Cephalosporins like Ceftriaxone, Ceftazidime and Cefotaxime were sensitive to Ciprofloxacin of various Enterobacteriaceae was only between 33-40% compared to 52.8-37.9% against Gentamicin. Majority of the Enterobacteriaceae were resistant to Ampicillin, whereas almost all of the Enterobacteriaceae (94-100%) were sensitive to Imipenem. About 97.0% Acinetobacter species were susceptible to Imipenem. Sensitivity of the organism (Acinetobacter) to third-generation Cephalosporins ranged between 50-56%, whereas 40.6% were found sensitive to Ciprofloxacin. The sensitivity to Chloramphenicol, Co-trimixazole, Cephalexin and Ampicillin ranged between 9.3% to 34.3%. About 93.0% of Pseudomonas species were sensitive to Imipenem. The rate of susceptibility to Gentamicin and Netilmicin was higher than those of the Ciprofloxacin and Ceftriaxone (37.8% and 53.5% vc. 39.2%). About 70% of isolated S. aureus were resistane of Oxacillin but all were sensitive to Vancomycin. The result of this study would help the physicians to make a judicious choice of antibiotics for therapeutic purposes.TAJ 2012; 25: 72-76