Antimicrobial Susceptibility Pattern of Bacterial Isolates from Wound Infection

Abstract

Objective: The present study was done to see the antimicrobial susceptibility pattern of bacterial isolates from wound infection.Methods: Wound swabs collected from 150 patients of wound infection were cultured and microbial isolates identified using standard methods. Antimicrobial susceptibility testing was done on bacterial isolates by Modified Kirby Bauer method.Results: Of the 150 swabs 131 (87.4%) were culture positive for bacterial pathogens, while 19 (12.6%) were bacteriologically sterile showing an isolation rate of 87.4%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%).In case of gram positive bacteria- rate of isolates resistant to ampicillin was 94%, followed by penicillin G, 86.8%, Tetracycline, 51.5%. In case of gram negative bacteria- rate of isolates resistant to ampicillin was 96%, followed by cephalothin, 92.4%, Tetracycline, 74%.Conclusion: In antimicrobial susceptibility testing ampicillin, penicillin, cephalothin and tetracycline were the least effective. Gentamicin, norfloxacin, ciprofloxacin, vancomycin and amikacin were the most effective antibiotics.TAJ 2013; 26: 61-66

Phenotypic Detection of Carbapenemase-producing WHO-declared Deadliest Drug-resistant Bacteria in the Rajshahi Region

“…S.aureus were the predominant bacteria with 71(30.8%) cases, followed by P.aeruginosa were 47(20.3%) cases, E. coli were 43(18.7%) cases, and Acinetobacter baumannii were 07(3%) cases. 20,21,22 Our study is about dissimilar to the study of Begum et al in Bangladesh and Khan et al in India. 23,24,25 The reason for this high occurrence of culture positivity may be due to the fact that most of the study population were belonged to lower middle and lower socioeconomic groups with poor knowledge about personal hygiene, flawed sanitation system in a hospital, overcrowding of patients in hospitals contribute to high rate of cross infection, inadequate measures for prevention of the spread of the resistant pathogen in a hospital environment.…”

“…Our study is nearly similar to the study of Nahar et al in Bangladesh and Saaiq, Ahmad and Zaib in Pakistan. 21,4 The failure to isolate an organism was 14.8% in the present study may be because the patients had received antibiotics, either systemic or topical or both before sample collection, or it may be that samples were collected from sites where there were no organisms, or there was the presence of anaerobic bacterial infection.…”