An Overview of the Isolation of Causative Agents from Wound Infection in A Tertiary Care Centre

Abstract

Objectives : To determine the prevalence, aetiology and susceptibility profile of bacterial agents of wound infection among in- and- out patients.Methods : Wound swabs collected from 150 patients were, cultured and microbial isolates identified using standard methods. Antibiotic susceptibility testing was done on bacterial isolates.Results : Of the 150 swabs 131 (87.4%) were culture positive for bacterial pathogens, while 19 (12.6%) were bacteriologically sterile showing an isolation rate of 87.4%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%). All isolates showed high frequency of resistance to ampicillin, penicillin, cephalothin and tetracycline. The flouroquinolones were the most potent antimicrobial agents against bacterial isolates from both in - and out -patients.Conclusion : Staphylococcus aureus was the most predominant etiologic agent of wound infection among in and out patients. A generally higher resistance pattern was observed among nosocomial bacterial pathogens. Prudent use of antibiotics is recommended.TAJ 2011; 24(2): 136-141

Phenotypic Detection of Carbapenemase-producing WHO-declared Deadliest Drug-resistant Bacteria in the Rajshahi Region

“…This higher infection cases in female patients may be due to poor nutrition, co-morbidity, malignancy, immunosuppression, and hematological disorders. 26 Our study is nearly similar to the study of Sharma et al in 34,9 Regarding Enterobacteriaceae, this is nearly similar to the study of Kaur et al in India and Diwakar et al in India. 28,35 But our study is nearly dissimilar to the study of Barai et al in Bangladesh and Pawar et al in India.…”

“…S.aureus were the predominant bacteria with 71(30.8%) cases, followed by P.aeruginosa were 47(20.3%) cases, E. coli were 43(18.7%) cases, and Acinetobacter baumannii were 07(3%) cases. 20,21,22 Our study is about dissimilar to the study of Begum et al in Bangladesh and Khan et al in India. 23,24,25 The reason for this high occurrence of culture positivity may be due to the fact that most of the study population were belonged to lower middle and lower socioeconomic groups with poor knowledge about personal hygiene, flawed sanitation system in a hospital, overcrowding of patients in hospitals contribute to high rate of cross infection, inadequate measures for prevention of the spread of the resistant pathogen in a hospital environment.…”

“…The prevalence of carbapenem resistant Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa in Bangladesh were 9.8%, 55% and 53.7% respectively, in India were 44.3%, 56% and 10% respectively and in US were 18%, 22% and 60.3% respective. 5,6,7,8,9,10 Regarding the antimicrobial resistance rates of Carbapenemase-producing Enterobacteriaceae in Bangladesh to third generation cephalosporins 80%-100%, fluoroquinolones, aminoglycosides, monobactam 60%-80% , to the carbapenem 10%-30%. 11,12 The antimicrobial resistance rates of Carbapenemase-producing Acinetobacter baumannii in Bangladesh to third-generation cephalosporins are 90%-100%, fluoroquinolones, aminoglycosides, monobactam 70%-80%, to carbapenem 25%-60%.The antimicrobial resistance rates of Carbapenemase-producing Pseudomonas aeruginosa in Bangladesh to thirdgeneration cephalosporins are 90%-100%, to fluoroquinolones, aminoglycosides, monobactam 65%-80%, to carbapenem 15%-30%.…”