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Regenerative medicine has received potential attention around the globe, with improving cell performances, one of the necessary ideas for the advancements of regenerative medicine. It is crucial to enhance cell performances in the physiological system for drug release studies because the variation in cell environments between in vitro and in vivo develops a loop in drug estimation. On the other hand, tissue engineering is a potential path to integrate cells with scaffold biomaterials and produce growth factors to regenerate organs. Scaffold biomaterials are a prototype for tissue production and perform vital functions in tissue engineering. Silk fibroin is a natural fibrous polymer with significant usage in regenerative medicine because of the growing interest in leftovers for silk biomaterials in tissue engineering. Among various natural biopolymer-based biomaterials, silk fibroin-based biomaterials have attracted significant attention due to their outstanding mechanical properties, biocompatibility, hemocompatibility, and biodegradability for regenerative medicine and scaffold applications. This review article focused on highlighting the recent advancements of 3D printing in silk fibroin scaffold technologies for regenerative medicine and tissue engineering.

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The Panchagavya is a combination of five products obtained from the cow which is used in traditional medicine and it has been experimented by various organic farmers.It has a significant role in providing Resistance to pests and disease and increasing the overall yield.The present research work was carried out to study the effect of panchagavya spray on growth,yield and Biochemical changes of Sesamum indicum. A field Experiment was conducted to find the variation in growth, Biochemical and yield parameters of Sesamum indicum Under different concentrations are control,1,3,5,7.5 and10% of panchagavya,and also at two stages of plant,where two stages are seedling and yielding stage and all the biochemical parameters were increased in 3% concentration Since there was an increasein growth and Yield at low concentration of panchagavya.It is proposed that,The panchagavya can be used for Sesamum indicum after diluted properly.

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