Tall fescue (Festuca arundinacea Schreb.) seedlings were grown in an intermittently irrigated gravel culture system in the greenhouse. Nutrient molybdate levels were 0, 0.1, or 0.21 μequiv./L with sulfate levels of 0 to 1.0 mequiv./L. Leaf tissue was harvested 28, 42, and 49 days after seeding. Molybdenum content of leaf tissue increased with increased solution Mo concentration. Increased solution SO42− levels increased tissue S and nitrate reductase activity (NRA), but decreased tissue Mo and NO3− content. When low tissue S was limiting NRA, and when Mo and NO3− were available, NRA was not increased by 2- to 4-h incubations of leaf tissue in SO42− solution. The MoO42− × SO42− interaction for tissue Mo concentration was significant and the effect of SO42− was relatively greater in the presence of Mo than in the absence of Mo.99Mo root uptake studies indicated the first increment of SO42− added to nutrient solution results in the greatest reduction in Mo accumulation. The data indicated that the greatest effect of SO42− on leaf Mo levels occurred during the absorption process with small reduction of Mo translocated from root to leaf tissue.
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