In the city of Magelang, Indonesia, the distribution of Dengue Haemorhagic Fever (DHF) cases tend to be clustered, ever changing along with human urbanization from 2014 to 2017. Although DHF cases have been less reported in the city of Magelang for the past 5 years, vector control measures by using insecticide space spraying, particularly permethrin, have been continuously performed. Current study aimed to detect kdr mutations associated with pyrethroid resistance in Ae . aegypti and to study possible association between insecticide resistance and DHF case distribution related to human urbanization. The study was a cross sectional study conducted in 3 sub-districts in the city of Magelang, Central Java, Indonesia. Eggs of Ae . aegypti collected from 195 sample households were reared and were tested for resistance to pyrethroids by using PCR. Primers AaSCF1 and AaSCR4, and primers AaSCF7 and AaSCR7 were used in detecting presence of mutation in VGSC IIS6 and IIIS6 gene, respectively. Fragments of amplified DNA were sequenced and were analyzed. Spatio-temporal using Standard Deviational Ellipse (SDE) was performed to obtain mapping of DHF case distribution trends. The total number of DHF case was 380 cases, with the most cases (158) occurred in 2015 and the least cases (66) reported in 2017. DHF case distribution was grouped into several clusters. SDE calculation demonstrated movement of DHF case in the direction to principal arterial road, suggesting link to urbanization. Gene sequencing demonstrated VGSC IIS6 gene mutation (S989P and V1016G) in Ae . aegypti collected from study areas, indicating resistance to permethrin. VGSC IIIS6 gene mutation was not found. Current study concluded that multiple kdr mutations associated with resistance to pyrethroid was detected in Ae . aegypti , and that human urbanization may have a role in the development of such resistance.
Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using egg white sedimentation and percoll density gradient centrifugation technique. Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratio. The obtained data were analyzed with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the sexing methods (non sexing sperm, upper and under fraction using percoll density gradient centrifugation, upper and under fraction using egg white sedimentation technique) had very significant effect (P<0.01) on motility (64.25±3.94%; 48.55±8.28%; 53±7.93%; 56.9±8.22%; 49.75±8.19% respectively) and significant effect (P<0.05) on the ratio of X and Y sperm. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation methods for the upper fraction were 28.0±2.26%:72.0±2.26% and 77.5±1.26%:22.5±1.26%. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation technique for the under fraction were 69.0±15.35%: 26.0±4.37% and 22.9±1.44%: 77.1±1.44%. The study concludes that the sexed with egg white sedimentation technique is better than percoll density gradient centrifugation on sperm motility and ratio of X and Y sperm.
ABSTRAKPenelitian ini bertujuan untuk mengetahui penampilan produksi sapi aceh di BPTU-HPT Indrapuri Aceh. Materi penelitian yaitu sapi aceh umur satu hari, umur sapih, dan umur satu tahun sebanyak 159 ekor. Metode penelitian adalah studi kasus berdasarkan data bobot lahir, bobot sapih, bobot satu tahun beserta ukuran tubuhnya. Data dianalisis menunjukkan bahwa sapi jantan memiliki bobot lahir 13,66±1,08 kg, bobot umur 205 hari 71,60±7,92 kg dan bobot umur 365 hari 104,66±11,72 kg. Sapi betina memiliki bobot lahir 13,88±1,32 kg, bobot umur 205 hari 64,38 ± 10,36 kg dan bobot umur 365 hari 90,29±11,95 kg. Sapi Aceh umur satu hari memiliki lingkar dada, panjang badan dan tinggi pundak berturut-turut 55,41 cm, 46,16 cm dan 55,22 cm pada sapi jantan serta 55,87 cm, 46,83 cm, dan 55,74 cm pada sapi betina. Sapi aceh umur 365 hari memiliki ukuran lingkar dada, panjang badan dan tinggi pundak berturut-turut 114,32 cm, 94,31 cm dan 92,93 cm pada sapi jantan serta 104,25 cm, 85,06 dan 87,71 cm pada sapi betina. Rata-rata penampilan produksi sapi aceh di BPTU Indrapuri berpotensi menjadi ternak unggul berdasarkan kriteria SNI.Kata Kunci: sapi aceh, penampilan produksi, bobot badan, ukuran tubuhABSTRACTThis study aimed to evaluate the productive performance of aceh cattle at Indrapuri Breeding Center for Superior Livestock and Forage. A total of 159 heads of aceh cattle were used in this study. The method used was a case study based on the data of birth weight, weaning weight, yearling weight, and body size. The results showed that the birth weight, weaning weight at 205 days of age, and yearling weight at 365 days of age of male aceh cattle were 13.66±1.08 kg, 71.60±7.92 kg, and 104.66±11.72 kg, respectively. Whereas, female aceh cattle had the birth weight, weaning weight at 205 days of age, and yearling weight at 365 days of age of 13.88±1.32 kg, 64.38±10.36 kg, and 90.29±11.95 kg, respectively. At birth, male Aceh cattle had the chest circumference, body length, and shoulder height of 55.41 cm, 46.16 cm, and 55.22 cm, respectively, while female aceh cattle were 55.87 cm, 46.83 cm, and 55.74 cm, respectively. At 365 days of age, male aceh cattle had the chest circumference, body length, and shoulder height of 114.32 cm, 94.31 cm, and 92.93 cm, respectively, while female aceh cattle were 104.25 cm, 85.06 cm, and 87.71 cm, respectively. The average productive performance of aceh cattle at Indrapuri Breeding Center for Superior Livestock and Forage has the potential to become superior cattle based on the criteria of Indonesian National Standard.Keywords: aceh cattle, performance, body weight, body size
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