-In the present study, the micromorphological structure of achene, pappus and style using scanning electron microscope (SEM), stomatal characteristics, anatomy of stem and achene together with chromosome number and nuclear DNA content of the Turkish endemic Crepis macropus Boiss. & Heldr. are provided in order to expand knowledge of its taxonomy. The SEM studies in this species show that dense spiny cells are found on the achene surface, the pappus bristle has 3-5 spikes and the style possesses slender papillae. The stem structure is composed of epidermis, collenchyma, parenchymatous cortex and pith. The species has anomocytic stomata in both the upper and the lower surface of the leaves. The pericarp of the achene is mainly composed of several layers of sclerenchymatous cells. In this species, the chromosome number is 2n = 2x = 8, karyotype consists of two submetacentric and six subtelocentric chromosomes and nuclear DNA content (2C-value) is 12.96 pg. These data are presented here for the fi rst time and their taxonomic values are discussed.
Achene anatomy and stomatal characteristics of eighteen Crepis taxa from Turkey are here described for the first time. In all taxa examined the pericarp is composed of several layers of sclerenchymatous and parenchymatous cells. As for the achene, differences among taxa mainly concern the pericarp structure and its thickness and width. Stomata are present on both surface of the leaf in all studied taxa and all taxa have anomocytic type stomata. However, the dimensions (length and width) and density of the stomata differ significantly among the studied taxa. In addition, the dimensions of stomata are negatively correlated with stomata density. It is concluded that achene anatomy and stomatal characteristics are useful for delimitation of Crepis taxa and a key to taxa based on these characters is provided. However, based on achene anatomy and stomatal characteristics, we found no argument for an exclusion of the Lagoseris group from Crepis as has previously been proposed.
Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz (Asteraceae) is a critically endangered endemic species in Turkey that is face the risk of extinction as a result of the fragmentation of its habitat as well as overgrazing and trampling of its natural population. However, the protocol for micropropagation of this threatened species has not been developed yet. Here, its regeneration ability on MS media supplemented with different plant growth regulators were evaluated using nodal segments. The higher number and length of shoot per explant was achieved with the addition 4.6 µM ZEA and 0.5 µM IAA to the culture medium. Besides, the highest node number of shoot per explant was obtained from MS medium supplemented with 4.6 µM ZEA and 0.5 µM IBA. Flow cytometric analysis also revealed that most of the in vitro developed shoots of T. insularum possessed similar nuclear DNA content as well as ploidy level as initial material and plants from natural population. In vitro rooting of shoots was achieved at 100 % e ciency containing 2.9 µM IAA. Rooted and well-developed plantlets were initially acclimatized under greenhouse conditions and then moved to the botanical garden, where they matured and owered. Finally, 76% and 74% survivals were achieved during the acclimatization process, respectively. This is the rst report of a successfully developed micropropagation protocol of threatened T. insularum for its ex situ conservation. et al. 2011;Valletta et al. 2015). Furthermore, the genetic diversity of local ecotypes can be maximized if plants are multiplied from seeds (Benson et al. 2000a;Coste et al. 2012).Tripleurospermum Sch.Bip. is a small genus of the tribe Anthemideae of the family Asteraceae with ca. 40 species that is mainly distributed in Europe, temperate Asia, North America and North Africa (Oberprieler et al. 2007). Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz is an insular endemic species with a highly restricted geographic range in Turkey, and is known from the single population in Gökçeada, one of the Aegean Islands (Inceer and Hayirlioglu-Ayaz 2014). It grows in open places and on rocky slopes in Gökçeada. On the other hand, T. insularum has a pharmaceutical potential (
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