In vitro propagation and cytogenetic stability of Tripleurospermum insularum (Asteraceae) – a critically endangered insular endemic species from Turkey

Abstract

Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz (Asteraceae) is a critically endangered endemic species in Turkey that is face the risk of extinction as a result of the fragmentation of its habitat as well as overgrazing and trampling of its natural population. However, the protocol for micropropagation of this threatened species has not been developed yet. Here, its regeneration ability on MS media supplemented with different plant growth regulators were evaluated using nodal segments. The higher number and length of shoot per explant was achieved with the addition 4.6 µM ZEA and 0.5 µM IAA to the culture medium. Besides, the highest node number of shoot per explant was obtained from MS medium supplemented with 4.6 µM ZEA and 0.5 µM IBA. Flow cytometric analysis also revealed that most of the in vitro developed shoots of T. insularum possessed similar nuclear DNA content as well as ploidy level as initial material and plants from natural population. In vitro rooting of shoots was achieved at 100 % e ciency containing 2.9 µM IAA. Rooted and well-developed plantlets were initially acclimatized under greenhouse conditions and then moved to the botanical garden, where they matured and owered. Finally, 76% and 74% survivals were achieved during the acclimatization process, respectively. This is the rst report of a successfully developed micropropagation protocol of threatened T. insularum for its ex situ conservation. et al. 2011;Valletta et al. 2015). Furthermore, the genetic diversity of local ecotypes can be maximized if plants are multiplied from seeds (Benson et al. 2000a;Coste et al. 2012).Tripleurospermum Sch.Bip. is a small genus of the tribe Anthemideae of the family Asteraceae with ca. 40 species that is mainly distributed in Europe, temperate Asia, North America and North Africa (Oberprieler et al. 2007). Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz is an insular endemic species with a highly restricted geographic range in Turkey, and is known from the single population in Gökçeada, one of the Aegean Islands (Inceer and Hayirlioglu-Ayaz 2014). It grows in open places and on rocky slopes in Gökçeada. On the other hand, T. insularum has a pharmaceutical potential (

Bitki Büyüme Düzenleyicilerinin Crepis bithynica (Asteraceae)’nın Sürgün Çoğaltımı Üzerine Etkileri: Türkiye’de Tehlike Altında Nadir Bir Tür

“…It is known that the species within Asteraceae have low germination rate in the achenes (Inceer et al, 2022). Similarly, the germination rate in the achenes of C. bithynica is relatively low in the in vitro culture.…”

“…The surface sterization of the achenes of C. bithynica was done with 70% ethanol and then soaked 3% sodium hypochlorite and finally washed with sterilized distillated water. Later, they were germinated on Murashige and Skoog (MS) (Murashige & Skoog, 1962) basal medium including vitamins supplemented with 4.7 µM kinetin (KIN) (Inceer et al, 2022).…”

“…Nodal explants were transferred to seven different MS media (Table 1). Culture conditions in the growth room were adjusted at 24 ± 2°C under a 16 h photoperiod at a photosynthetic flux density of 50 μmol m −2 s −1 with cool daylight fluorescent lamps (Inceer et al, 2022).…”

In Vitro Propagation and Phytochemical Composition of Centratherum punctatum Cass—A Medicinal Plant

“…Bip.) [40]. IAA, a naturally occurring auxin, has previously been considered to be crucial for in vitro rooting as well as in the plant regeneration process [41], as was demonstrated in Alocasia longiloba.…”