In this study, the antimutagenic effect of Rosa canina (rosehip) infusion against to mutagenic effect of the herbicide linuron was investigated on meristematic root cells of Allium cepa (onion) by Allium test and flow cytometry method. In this context, firstly, roots of A. cepa were treated with 0.1 mg mL 1 linuron for 12 and 24 h. Then the roots were recovered by R. canina infusion for 12 and 24 h. In a result of Allium test, depending on the exposure time of linuron, it was determined linuron suppressed to mitosis in meristematic root cells and caused disturbed chromatin, sticky chromosomes, c-metaphase, chromosome bridges, vagrant chromosome abnormalities in meristematic root cells. In addition, linuron was found to inhibit DNA synthesis in S-phase of the cell cycle with flow cytometric analysis. On the other hand, it was found that the infusions of R. canina have activated DNA synthesis, arranged mitotic cell division, and reduced chromosomal abnormalities of linuron. Thus, protective effect and healing of the negative effects in mitotic cells of R. canina infusions against to the linuron toxicity on cell division was revealed.
Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz (Asteraceae) is a critically endangered endemic species in Turkey that is face the risk of extinction as a result of the fragmentation of its habitat as well as overgrazing and trampling of its natural population. However, the protocol for micropropagation of this threatened species has not been developed yet. Here, its regeneration ability on MS media supplemented with different plant growth regulators were evaluated using nodal segments. The higher number and length of shoot per explant was achieved with the addition 4.6 µM ZEA and 0.5 µM IAA to the culture medium. Besides, the highest node number of shoot per explant was obtained from MS medium supplemented with 4.6 µM ZEA and 0.5 µM IBA. Flow cytometric analysis also revealed that most of the in vitro developed shoots of T. insularum possessed similar nuclear DNA content as well as ploidy level as initial material and plants from natural population. In vitro rooting of shoots was achieved at 100 % e ciency containing 2.9 µM IAA. Rooted and well-developed plantlets were initially acclimatized under greenhouse conditions and then moved to the botanical garden, where they matured and owered. Finally, 76% and 74% survivals were achieved during the acclimatization process, respectively. This is the rst report of a successfully developed micropropagation protocol of threatened T. insularum for its ex situ conservation. et al. 2011;Valletta et al. 2015). Furthermore, the genetic diversity of local ecotypes can be maximized if plants are multiplied from seeds (Benson et al. 2000a;Coste et al. 2012).Tripleurospermum Sch.Bip. is a small genus of the tribe Anthemideae of the family Asteraceae with ca. 40 species that is mainly distributed in Europe, temperate Asia, North America and North Africa (Oberprieler et al. 2007). Tripleurospermum insularum Inceer & Hayirlioglu-Ayaz is an insular endemic species with a highly restricted geographic range in Turkey, and is known from the single population in Gökçeada, one of the Aegean Islands (Inceer and Hayirlioglu-Ayaz 2014). It grows in open places and on rocky slopes in Gökçeada. On the other hand, T. insularum has a pharmaceutical potential (
Aim of study: In this study, the effects of plant growth regulators (PGRs) on shoot multiplication of Crepis bithynica Boiss. (Asteraceae), an endangered rare species in Türkiye were investigated.
Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park.
Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog (MS) basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures.
Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively.
Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.
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