Micromorphological, Anatomical and Cytogenetical Studies in Endemic Crepis macropus Boiss. & Heldr. (Asteraceae) from Turkey

Abstract

-In the present study, the micromorphological structure of achene, pappus and style using scanning electron microscope (SEM), stomatal characteristics, anatomy of stem and achene together with chromosome number and nuclear DNA content of the Turkish endemic Crepis macropus Boiss. & Heldr. are provided in order to expand knowledge of its taxonomy. The SEM studies in this species show that dense spiny cells are found on the achene surface, the pappus bristle has 3-5 spikes and the style possesses slender papillae. The stem structure is composed of epidermis, collenchyma, parenchymatous cortex and pith. The species has anomocytic stomata in both the upper and the lower surface of the leaves. The pericarp of the achene is mainly composed of several layers of sclerenchymatous cells. In this species, the chromosome number is 2n = 2x = 8, karyotype consists of two submetacentric and six subtelocentric chromosomes and nuclear DNA content (2C-value) is 12.96 pg. These data are presented here for the fi rst time and their taxonomic values are discussed.

Achene anatomy and stomatal characteristics of eighteen Crepis L. (Asteraceae) taxa from Turkey with notes on their systematic significance

“…3-4). These characteristics agree with what has previously been reported by Metcalfe and Chalk (1979), Enke (2009), Jana and Mukherjee (2012), Karaismailoglu (2015) and Inceer et al (2016). However, our results on achene anatomy also show that there are considerable differences in the pericarp among the studied taxa.…”

“…The present results show that all taxa have anomocytic stomata. This stoma type has previously been reported in C. macropus (Inceer et al 2016) and other members of the Asteraceae family (Adedeji and Jewoola 2008, Inceer and Ozcan 2011, Ozcan et al 2015.…”

“…Anatomical observation was performed on the achenes. For this purpose, transverse sections of the achene were prepared with the paraffin method (Algan 1981) and then stained with hematoxylin and mounted in entellan (Inceer et al 2016). Five slides from five individuals were prepared from each sample.…”

“…Among the taxonomically important characteristics of stomata is the arrangement of the surrounding epidermal cells, size and density, as well as the stomatal index. However, there are only a few taxonomic studies on stomata of Crepis, and they are limited to a few representatives of the genus (Gupta et al 2012, Inceer et al 2016.…”

A new subspecies of <i>Crepis palaestina</i> (Asteraceae) from Turkey

“…They were hydrolyzed in 1N HCl at 60 o C for 12-15 min. Staining was carried out in 1% lacto-propionic orcein for 12-18 h at room temperature and squash preparations were made in 45% acetic acid (Inceer et al, 2016). Five well-spread metaphase plates were used for chromosome counts.…”