Despite advances in the prevention and treatment of hypertension over the past decade, hypertension remains an important public health challenge. Recent efforts to reduce the prevalence of hypertension have focused on nonpharmacologic means, specifically diet. An increased intake of minerals such as potassium, magnesium, and calcium by dietary means has been shown in some but not all studies to reduce blood pressure in patients with hypertension. This review will discuss the roles of potassium, magnesium, and calcium in the prevention and treatment of essential hypertension with specific emphasis on clinical trial evidence, mechanism of action, and recommendations for dietary intake of these minerals. A high intake of these minerals through increased consumption of fruits and vegetables may improve blood pressure levels and reduce coronary heart disease and stroke. J Clin Hypertens (Greenwich). 2008;10(7 suppl 2):2–11.

Abstract:The intake and digestibility of forages is largely influenced by the fibre content and specifically the neutral detergent fibre (NDF). Currently, the focus in commercial diet formulation and the modelling of animal performance is on the total NDF so as to achieve higher ruminant feed intakes, higher production performance and rumen health. Rations are often formulated for a specific level of NDF in the diet assuming that the digestibility of NDF operates over a narrow range. Forage NDF, particularly in C4 forages, varies greatly in potential digestibility within the rumen. This potential digestibility is defined as the NDF fraction which disappears after a long incubation period and the remaining indigestible component of NDF (iNDF) is unavailable for microbial digestion. It is hypothesized that this dietary iNDF has an important role in contributing to rumen digesta load and voluntary intake. Formulating a diet to a specific level of NDF without reference to the iNDF could markedly affect the resulting intake, digestibility and metabolisable energy (ME) content of the diet. It is concluded that nutritional models need to be modified to accept directly determined iNDF.

A spore-forming probiotic, Bacillus amyloliquefaciens strain H57 (H57), was administered to dairy calves in starter pellets to determine effects on liveweight gain, feed conversion efficiency and animal health under summer feeding conditions, without antibiotics. Twenty-four male and female calves were allocated into two groups and from 4 weeks of age individually offered 6 L/day of whole milk and ad libitum starter pellets impregnated with H57 (3.16 × 108 cfu per kg DM) or without (Control) until 12 weeks of age. The calves were housed in a non-air-conditioned animal house, with deep-straw bedding over concrete, under typically challenging subtropical summer conditions. After 12 weeks the calves were released into a grazing paddock as one group and were supplemented ad libitum with control pellets and hay, until 19 weeks of age. From Weeks 4 to 12, liveweight and feed intakes were measured weekly and health status was monitored daily. Rumen fluid and blood were collected at Weeks 4 and 12, and to test for persistence after cessation of feeding H57, each were measured again at Week 19. From Weeks 4 to 12, the H57 calves grew faster (767 vs 551 g/day, P = 0.01), tended to consume more pellets (1013 vs 740 g DM/day, P = 0.07) and were 19% more feed conversion efficiency (2.43 vs 2.90 kg milk + starter DM/kg weight gain, P = 0.01) compared with the Control calves. The mean duration of each diarrhoea event was 2 days less for the H57 calves than the Control (P = 0.01). The H57 calves weaned 9 days earlier (P = 0.02) and were heavier at Week 19 (155 vs 139 kg, P = 0.03) than the Control calves. The only effect of H57 on rumen volatile fatty acid concentrations was an elevation in valerate at Week 12 (4.10 vs 2.47 mmol/L, P = 0.03). Plasma β-hydroxy butyrate was also elevated in the H57 calves at Week 19 (0.24 vs 0.20 mmol/L), indicating the potential of H57 to improve rumen development. H57 can be used to improve the nutritional performance and reduce the risk of diarrhoea in dairy calves as they transition from milk to dry feed.

Two experiments evaluated the effects of a Bacillus-based direct-fed microbial on in vitro dry matter (DM) and neutral detergent fiber (NDF; Exp. 1), and starch (Exp. 2) digestibility of a variety of ruminant feedstuffs. In Exp. 1, ten forage sources were evaluated: ryegrass, alfalfa hay, leucaena, corn silage, spinifex, buffel grass, flinders grass, Mitchell grass, rhodes grass hay, and Queensland bluegrass. Experimental treatments were a Control, forages with no probiotic inoculation; CON) or forages sources inoculated with a mixture containing Bacillus licheniformis and B. subtilis (3.2 × 10 9 CFU/g; DFM). In vitro DM and NDF digestibility were evaluated at 24- and 48-hours post-treatment inoculation. Treatment × hour interactions were noted for IVDMD and IVNDFD (P ≤ 0.05). More specifically, DFM inoculation increased (P ≤ 0.03) IVDMD at 24 hours in four forages and increased 48-hour IVDMD (P ≤ 0.02) in alfalfa hay, ryegrass, leucaena, and Mitchell grass hay, but opposite results were observed for Queensland bluegrass (P < 0.01). Twenty-four and 48-hour IVNDFD increased following DFM inoculation (P ≤ 0.02) in five forage sources, but reduced for Queensland bluegrass (P < 0.01). When the forages were classified according to their quality, main treatment effects were detected for IVDMD (P ≤ 0.02) and IVNDFD (P < 0.01). In Exp. 2, five common cereal grains were evaluated [high-density barley (82 g/100 mL), low-density barley (69 g/100 mL), corn, sorghum, and wheat] under the same treatments as in Exp. 1. In vitro starch digestibility was evaluated at 6- and 12-hours following treatment inoculation. Treatment × hour interactions were observed for starch digestibility in 3 out of 5 concentrate sources (P ≤ 0.001). Inoculation of DFM yielded greater 24-hour starch digestibility for high-, low-density barley, and wheat (P ≤ 0.02), but also greater at 48 hours in wheat (P < 0.0001). Moreover, mean starch digestibility improved for corn and sorghum inoculated with DFM (P < 0.01). Using a Bacillus-based DFM (B. licheniformis and B. subtilis) improved mean in vitro DM and NDF digestibility of different forage sources of varying qualities (based on CP content). Similarly, in vitro starch digestibility was also greater following DFM inoculation, highlighting the potential of this probiotic to improve nutrient digestibility and utilization into the beef and dairy cattle herd.

Biopesticides are biological pest control agents that are viewed as safer alternatives to the synthetic chemicals that dominate the global insecticide market. A major constraint on the wider adoption of biopesticides is their susceptibility to the ultraviolet (UV: 290-400 nm) radiation in sunlight, which limits their persistence and efficacy. Here, we describe a novel formulation technology for biopesticides in which the active ingredient (baculovirus) is micro-encapsulated in an ENTOSTAT wax combined with a UV absorbant (titanium dioxide, TiO 2). Importantly, this capsule protects the sensitive viral DNA from degrading in sunlight, but dissolves in the alkaline insect gut to release the virus, which then infects and kills the pest. We show, using simulated sunlight, in both laboratory bioassays and trials on cabbage and tomato plants, that this can extend the efficacy of the biopesticide well beyond the few hours of existing virus formulations, potentially increasing the spray interval and/or reducing the need for high application rates. The new formulation has a shelf-life at 30 °C of at least 6 months, which is comparable to standard commercial biopesticides and has no phytotoxic effect on the host plants. Taken together, these findings suggest that the new formulation technology could reduce the costs and increase the efficacy of baculovirus biopesticides, with the potential to make them commercially competitive alternatives to synthetic chemicals. Baculoviruses are dsDNA viruses that infect insects and have, since the 1980s, been used in crop protection as commercial biological insecticides 1,2. Baculoviruses are seen as attractive biological control agents against insect crop pests for many reasons: they have a long and detailed history of research, so basic knowledge of their taxonomy, biology and pathogenicity is available 3 ; they have an established profile of safety and environmental acceptability 4 ; they are highly efficacious pathogens of some of the world's most important crop pests, such as the various Heliothis / Helicoverpa species, Spodoptera spp. and Plutella xylostella 5 ; and, finally, their use as biological pesticides is feasible because commercially-viable mass production systems are well advanced for many baculoviruses 6. These factors have motivated the establishment of a growing commercial production of baculovirus insecticides in the Americas, Europe, Asia, Australasia and Africa 5,7. Moreover, biopesticides are now seen as a major candidate for replacing the many chemical pesticides that have been, and continue to be, withdrawn from the market due to safety concerns 5,8 , and/or where the insect pests have developed resistance to conventional chemical pesticides 9. Baculovirus products, however, still represent only a $50-70 million per annum sector of a global biopesticides market estimated to be worth $2.8 billion dollars a year 10. While a number of factors have been identified as restricting the adoption and expansion of the use of baculovirus biopesticides by growers 5 , a centr...

Keynote paper presented at the International Leucaena Conference, 1‒3 November 2018, Brisbane, Queensland, Australia.Leucaena can be fed as the sole diet to fattening cattle without nutritional problems and it will promote high liveweight gains. The high crude protein concentration in leucaena suggests that energy supplements, which are readily fermented in the rumen, could be used to capture the excess rumen degradable protein and provide more microbial protein and metabolizable energy to the animal, further increasing liveweight gain or milk production. This approach has been tested in grazing cattle and also in cut-and-carry systems in Australia and Indonesia. In both systems, production (liveweight gain or milk production) increased with the addition of supplements containing large amounts of fermentable meta- bolizable energy. The substitution of the basal diet (leucaena or leucaena mixed with grass or crop residues) by the supplement also means that more animals can be carried in the system for a set amount or area of leucaena. The same principles would apply to any tree legume-based system. Energy supplements can come in many forms, viz. fermentable starch (cereal grains and cassava), sugars (molasses), pectins (soybean hulls and pulps) and fibre (rice bran, cassava bagasse), but they have not been compared for their efficacy nor for their economic benefit, if any, in these systems.

Context Formulating rations with high energy and protein feeds such as cassava tuber and gliricidia, is an important strategy to increase liveweight gain (LWG) of bulls and improve profitability of smallholder farmers in Indonesia. Aims Two on-farm experiments were conducted to evaluate the effect of increasing the supplementation level of a mixture of cassava (Manihot esculenta) whole root tuber powder and fresh gliricidia (Gliricidia sepium) on feed intake and LWG of Ongole and Bali bulls given fresh corn stover and elephant grass, respectively. Methods Two experiments were conducted, each with five treatments: a basal diet of fresh corn stover (Experiment 1 for Ongole bulls) or elephant grass (Experiment 2 for Bali bulls) fed ad libitum or this diet supplemented with a combination of cassava tuber (whole root tuber including peel, sun-dried and ground) and fresh, chopped gliricidia (1:1) on estimated dry matter (DM) basis, at DM levels of 0.4, 0.8, 1,2 and 1.6% liveweight (LW)/day. Each experiment was run for 18 weeks, consisting of a two week adaptation and 16 week experimental period. Parameters measured included basal DM intake, supplement DM intake, total DM intake, total water intake, faecal pH, daily LWG, feed conversion ratio (FCR) and income over feed cost (IOFC). Key results Increasing supplement level linearly (P < 0.05) decreased basal diet intake, but linearly increased total DM intake and LWG (P < 0.05) in both breeds. Basal diet DM intake was reduced at the rate of 0.43 kg/kg of supplement DM consumed for both Ongole and Bali bulls. Water intake and faecal pH were not affected (P > 0.05) by increasing supplement intake. Inclusion of cassava tuber and gliricidia supplement up to 1.6% LW/day, increased total feed DM intake (up to 3.28 and 3.18% LW/day, for Ongole and Bali bulls, respectively) and LWG (maximum Ongole bulls 0.69 kg/day and Bali bulls 0.46 kg/day). Daily income over feed cost (IOFC) increased significantly (P < 0.05) in association with increased supplement intake in both Experiments 1 and 2. Using the derived model without supplementation, the value of IOFC was only IDR 7802/day and IDR 7687/day, for Experiments 1 and 2, respectively. The highest IOFC was achieved at a supplement intake of 1.6% LW/day with values of IDR 13949/day and IDR 12543/day for Experiments 1 and 2 respectively. Conclusions The addition of a cassava tuber and gliricidia mixture up to 1.6% LW/day increased LWG and profit for smallholders fattening bulls. Implications Formulating a ration with cassava tuber and gliricidia can be economically beneficial in cattle fattening systems in Indonesia.

No abstract