The present study was conducted on the incidence studies of the canine parvovirus (CPV) infection from different parts of Andhra Pradesh, India. A total of three hundred and forty two (n=342) canine parvovirus suspected clinical faecal samples of different age group of dogs from both vaccinated and non-vaccinated animals were collected. The faecal samples were preliminarily screened by haemagglutination assay and further confirmation by employing polymerase chain reaction (PCR). Dogs of age group below four months were more susceptible for CPV infection at a higher rate (77.59%) and were predominantly seen in males. PCR technique was found to be more sensitive in confirming the CPV infection (68.42%) than haemagglutination assay (20.67%).
Ovine pulmonary adenocarcinoma (OPA) caused by an exogenous Jaagsiekte sheep retrovirus (JSRV) is prevalent in Indian sheep. In the present study, OPA was diagnosed in sheep by clinical signs, gross and histopathology and polymerase chain reaction (PCR). Proviral DNA of exogenous JSRV was detected in lung tumor tissues, mediastinal lymph nodes, blood and lung fluid samples from natural cases of OPA by using U3-hn PCR and the PCR amplicons were sequenced to analyze nucleotide divergence. In total, six isolates were sequenced that had 96%-100% homology with a UK strain (AF105220.1) but more divergent from a South African strain (M80216) with 88%-93% identity. The phylogenetic analysis revealed segregation of the six isolates into two clusters. In conclusion, this study is the first report on sequencing and phylogenetic analysis of JSRV in India and further studies are suggested to know the complete sequencing and genetic divergence of JSRV in Indian sheep.
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