Incidence of canine parvovirus infection of dogs in Andhra Pradesh

Abstract

The present study was conducted on the incidence studies of the canine parvovirus (CPV) infection from different parts of Andhra Pradesh, India. A total of three hundred and forty two (n=342) canine parvovirus suspected clinical faecal samples of different age group of dogs from both vaccinated and non-vaccinated animals were collected. The faecal samples were preliminarily screened by haemagglutination assay and further confirmation by employing polymerase chain reaction (PCR). Dogs of age group below four months were more susceptible for CPV infection at a higher rate (77.59%) and were predominantly seen in males. PCR technique was found to be more sensitive in confirming the CPV infection (68.42%) than haemagglutination assay (20.67%).

First Isolation of Highly Cytopathic Canine Parvovirus-2 on Madin Darby Canine Kidney Cells from a Diarrheic Pet Dog of Indore in Madhya Pradesh and its Molecular Characterization as Canine Parvovirus-2a

“…In the present study, the CPV-2 viral isolate required only one passage on MDCK cells for its adaptation and production of CPE indicative of the highly cytopathic nature of isolated CPV-2. CPV-2 and its variants are being reported in India (Kaur et al, 2015;Sharma et al, 2018;Kumari et al, 2019). The highly cytopathic CPV-2 strain was further characterized at its molecular level as CPV-2a in the amplification refractory mutation system-polymerase chain reaction test (Chander et al, 2016) (National Centre for Biotechnology Information Accession Number: MZ209401).…”