The present study was conducted on the incidence studies of the canine parvovirus (CPV) infection from different parts of Andhra Pradesh, India. A total of three hundred and forty two (n=342) canine parvovirus suspected clinical faecal samples of different age group of dogs from both vaccinated and non-vaccinated animals were collected. The faecal samples were preliminarily screened by haemagglutination assay and further confirmation by employing polymerase chain reaction (PCR). Dogs of age group below four months were more susceptible for CPV infection at a higher rate (77.59%) and were predominantly seen in males. PCR technique was found to be more sensitive in confirming the CPV infection (68.42%) than haemagglutination assay (20.67%).
The present study was aimed for the confirmatory diagnosis of contagious agalactia syndrome in sheep herds belonging to Ibrahimpatnam and Mylavaram mandals of Krishna district, Andhra Pradesh. A total of 37 clinical samples were collected from the affected sheep with a history of keratoconjuctivitis, corneal opacity, arthritis, anorexia and mastitis without any reports of mortality. The symptoms of the affected sheep were preliminarily suggestive of Mycoplasmal infections. An attempt was made for confirming the organism based on genotypic characterization by employing molecular based technique, polymerase chain reaction using mycoplasma group specific primers for a quick diagnosis. All the 37 samples were processed for DNA extraction by employing high salt DNA extraction method and phenol chloroform method. Amplification of the mycoplasma gene produced an amplicon product size of 270bp indicating 100% positivity to Mycoplasma infection among the herd.
The present study aimed to assess the presence of Canine parvovirus 2 (CPV-2) antibodies in both vaccinated and unvaccinated dogs. The canine parvovirus disease in dogs was monitored by the use of live attenuated and killed vaccines besides new generation vaccines were also being developed for the control strategy. A total of 542 serum samples from different regions of Andhra Pradesh were used for the study. The age, sex, breed, and vaccination status were noted. The seroprevalence of canine parvovirus infection was studied by employing the methods of indirect ELISA and haemagglutination inhibition (HI) assay. The CPV infection rate was recorded as 94.65% in vaccinated and 72.50% in unvaccinated by indirect ELISA. Haemagglutination inhibition assay could detect 56.10% in vaccinated and 16.42% in unvaccinated ones. The titres ranged from 419 to 2337. Vaccinated dogs had more antibodies than the unvaccinated ones thus indicating the presence of antibodies in all the dogs suggesting that CPV infection is ubiquitous and natural infection of CPV aids in boosting immunity.
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