Molecular characterization and phylogenetic analysis of canine parvovirus strains in Andhra Pradesh, India

Kumari, G. Deepika; Pushpa, R.; Subramanyam, K.V.; Rao, T. S.; Satheesh, K.; Srinivas, Vivek

doi:10.36062/ijah.60.1.2021.90-97

Cited by 1 publication

(2 citation statements)

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“…Discussions Among 203 suspected dogs, 162 were found to be positive for CPV infection by PCR assay, with an overall positivity rate of 79.8% in this study. Similar results were also found in our earlier studies [11,12] and other studies from other researchers in India [17,29]. Out of seventy four recent CPV strains sequenced, an overwhelming majority were characterized as CPV-2c strain (79.73%) followed by CPV-2a variant in 18.9% CPV samples and CPV-2b variant in just one sample (1.35%).…”

Section: B Cell Epitope Predictionsupporting

confidence: 90%

“…Interesting this set of CPV-2c strains were grouped away from the current Indian CPV vaccine strains. In clade II, very few recent CPV-2c strains without 370th variation were grouped along with recent CPV-2a/ 2b, repository isolates (CPV-2a/2b variant) as well as with the Chinese CPV-2a variants which were in agreement with the earlier ndings [18,29]. Some Recent CPV-2c strains without 324th variation (with prototype Tyr324) were formed a separate clade (clade III) with the prototype CPV-2c strain and vaccine strains.…”

Section: B Cell Epitope Predictionsupporting

confidence: 89%

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Whole genome sequence analysis of canine parvovirus reveals pan-Indian replacement with Novel CPV-2c strain

Reddy,

Mukhopadhyay,

Srini

et al. 2024

Preprint

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Canine parvovirus (CPV) infection causes severe gastroenteritis in canines with high mortality in puppies. This virus evolved from FPV by altering its transferrin receptor (TfR) followed by emergence of CPV-2 variants in subsequent years by altering the immunodominant VP2 amino acid residues. Though the previous studies analysed VP2 gene, there are limited studies on non-structural protein (NS1 & NS2) genes. In this study, comparative whole genomic analysis was performed with the CPV suspected clinical samples collected Pan-India in 2023 with the repository CPV strains (2009–2019 isolates) and the current Indian CPV vaccine strain. The present pan-Indian study showed that the CPV-2c (N426E) had almost completely replaced the previously dominant CPV-2a variant (N426) in India. The Q370R mutation of VP2 was the most common change in the recent CPV-2c strain (CPV-2c 370Arg variant). Phylogeny showed the existence of three clades among the recent CPV-2c strains. The study identified several new positive selection sites in VP1 (N terminus), VP2, NS1 and NS2 genes in the recent CPV strains indicating emergence of new CPV-2c variants with varied antigenic and replication properties. The predominant ‘CPV-2c 370Arg variants’ were grouped along with the Chinese and Nigerian CPV-2c strains away from the CPV vaccine strain and repositories. VP2 epitope analysis predicted nine amino acid variations (includes 2 new variations) at four potential linear B-cell epitopes in the ‘CPV-2c 370Arg variants’ underlying chance of vaccine failure. This pan-Indian study, laid the foundation for further research concerning the dynamics of virus evolution and understanding genetic mutations.

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