This study was carried out to determine the cutoff levels of serum pepsinogen (PG) I , II and their ratio of PG I /PG II for gastric cancer to establish a better screening system. Optimal cutoff levels for gastric cancer screening using serum pepsinogens were determined using Youden's index. The sensitivity, specificity and Youden's index for gastric cancer cases were calculated according to sex, age and the stage of gastric cancer, and the maximum Youden's index in each category was adopted as the cutoff level for gastric cancer screening using serum pepsinogens. The maximal Youden's index in all gastric cancer cases was 0.37, corresponding to a cutoff level of PG I < 40 (* g/I) and PG I /PG II < 3.5. The sensitivity and specificity for gastric cancer cases of these cutoff levels were 0.50 and 0.87, respectively. In future, better criteria for gastric cancer screening have to be examined with the estimation of Youden's index in addition to other epidemiological methods such as ROC (receiver operating characteristic) curves and/or cost benefit analyses.J Epidemiol, 1997 ; 7 : 143-151.gastric cancer screening, serum pepsinogens, cutoff levelsThe mortality rate of gastric cancer is decreasing in Japan.However, cancer is the most common and second most common cause of death in Japanese men and women, respectively 1) . The risk factors for gastric cancer are considered to be high dietary salt intake, shortage of fresh fruit and vegetables in the diet, smoking and aging 4. Chronic atrophic gastritis has recently been shown to be a precancerous lesion 3.4.5). As serum pepsinogens (PG I PG II) and the ratio of PG I /PG II are correlated with atrophic gastric mucosa lesions, serum pepsinogena I and the ratio of PG I /PG II have been used to screen for gastric cancer instead of indirect or direct X-ray examination in some medical facilities in Japan6,7,8 9). The method using serum pepsinogens is very useful to detect well-differentiated adenocarcinomas in the stomach because low PG I and low PG I /PG II are highly correlated with such lesions10,11) However, the cutoff level of serum pepsinogen used for screening of gastric cancer varies among different medical facilities 6,7,8,9). It is thus necessary to determine the optimal cutoff levels for gastric cancer screening by this method.This study was carried out to determine the optimal cutoff level for gastric cancer screening. We examined 123 gastric cancer cases collected from cooperating hospitals in Oita prefecture, and 97 subjects from the same hospitals in whom no gastrointestinal lesions were detected by gastrointestinal fiberscopy at regular medical checkup examination. The parameters related to serum pepsinogens were examined according to sex, age, stage of gastric cancer, and then the distributions of these parameters were characterized. Further, the optimal cutoff level for gastric cancer screening using the serum pepsinogens was determined as the value that yielded the maximum Youden's index"). The cutoff level given by this method was compared with those deter...