Fast and innovative methodology to monitors the addition of soybean oil in extra virgin olive oil was developed employing ESI-MS with ionization operating in positive mode. A certified extra virgin olive oil and refined soybean oil samples were analyzed by direct infusion, the identification of a natural lipid marker present only in soybean oil (m/z 886.68 [TAG+NH]) was possible. The certified extra virgin olive oil was purposely adulterated with soybean oil in different levels (1, 5, 10, 20, 50, 70, 90%) being possible to observe that the new methodology is able to detect even small fraud concentration, such as 1% (v/v). Additionally, commercial samples were analyzed and were observed the addition of soybean oil as a common fraud in this segment. This powerful analytical method proposed could be applied as routine analysis by control organization, as well as food industries, considering its pronounced advantages; simplicity, rapidity, elevated detectability and minor amounts of sample and solvent consumed.

Total phenolic content (TPC), total flavonoids (TF), total anthocyanins (TA), and antioxidant capacity of different parts of four unexplored fruits from Brazil (Syzygium cumini Lam, Solanum nigrum Linn, Inga edulis Mart, and Hovenia dulcis Thunb) were determined; the bioactive compounds and fatty acids were quantified and identified by high-performance liquid chromatography and gas chromatography, respectively. S. cumini peels contained the most TA (63.31 mg/100 g), whilst H. dulcis pulp and peels had the highest TPC (518.18 mg GAE/100 g) and TF (76.54 mg EQ/g). Phenolic compounds responsible for antioxidant capacity of fruits were gallic acid, ellagic acid, kaempferol, and epicatechin. H. dulcis seed showed the highest level of the essential fatty acid omega-3 (3985.95 mg/100 g). PCA showed that PC1 and PC2 explained 90.43% of the total variability of the antioxidant data. Most of the seeds showed omega-3, omega-6, and omega-9 fatty acids at significant concentrations, with two PCs explaining 93.80% of the total variance of the fatty acid contents.

No abstract

This study identified and quantified five phenolic compounds, and evaluated the antioxidant capacity in vitro of fifteen native and exotic Brazilian fruit seeds that are typically discarded as waste. The contents of phenolic compounds were determined by ultra-performance liquid chromatography coupled with tandem mass spectrometry, and the antioxidant capacity was determined by oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH•) antioxidant assays. The results showed the antioxidant activity of Campomanesia pubescens, Hovenia dulcis Thunberg and Syzygium jambos (L.) Alston in the ORAC assay, and Hymenaea stigonocarpa, Hovenia dulcis Thunberg and Campomanesia pubescens in the DPPH• assay. Among the fifteen samples, four were highlighted regarding phenolic compound analyzes: Hovenia dulcis Thunberg (5.723 µg g-1) for gallic acid and myricetin (111.057 µg g-1), Passiflora edulis (1.208 µg g-1) for chlorogenic acid, Annona atemoya (1.0580 µg g-1) for vanillic acid, and Campomanesia pubescens (0.420 µg g-1) for ferulic acid. Therefore, these fruit seeds can be used as alternative sources of natural antioxidants.

The objective was identifying three different anthocyanidins and to quantify them in six different berries. Results from the conventional method (using extraction with hydrochloric acid) were compared with data from the proposed method (without acid extraction). The following figures of merit were used: linearity, accuracy, linear range, limits of detection and quantification, using an ultra performance liquid chromatography (UPLC) coupled to a triple quadrupole mass spectrometer equipped with an electrospray ionization source. The separation of anthocyanidins can be achieved in a much smaller period of time (1.30 min) and the cyanidin from Morus nigra L., Rubus idaeus L. and Vaccinium myrtillus L. was found in higher amounts in extracts obtained from the proposed method. Proposed mechanisms of mass spectrometry (MS/MS) collisional induced dissociation for pelargonidin, cyanidin and delphinidin were obtained and this is the first time that concentrations of anthocyanidins present in fruits of Solanum americanum Mill. were reported.

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Solanum americanum Mill. is a plant that belongs to the Solanaceae family, its respective ripe fruit is dark purple. Ripe S. americanum Mill. fruits were submitted to physicochemical characterisation, and their trans-resveratrol contents were quantified by high-performance liquid chromatography. Such determination was executed with fruits at different stages of ripeness and freeze-stored fruits as well. In natura ripe fruit pulp and peel presented average trans-resveratrol amounts of 1.07 and 0.7960 μg per gram of sample, respectively. These amounts are significantly higher when compared to freeze-stored fruit (0.1353 μg of trans-resveratrol per gram of sample) and to other berries. All ripe fruits showed significant amounts of total anthocyanins and total antioxidants. Thus, for the first time, trans-resveratrol has been identified and quantified in S. americanum Mill. fruit samples.

O objetivo foi testar duas formulações de esfirra integral, uma com adição de aveia e outra sem adição de aveia, ambas com recheio de proteína texturizada de soja, além de realizar a pesquisa de mercado, identificar a porção recomendada, o valor calórico, o índice de aceitação e a análise sensorial. A análise sensorial foi realizada com 117 provadores não treinados, os atributos avaliados foram aroma, sabor, textura, aparência global e intenção de compra. O índice de aceitação para as duas formulações de esfirras foi maior que 70%, sendo que não foi observada a rejeição dos produtos avaliados. A composição centesimal demonstrou esfirras com bom aporte protéico e de fibra alimentar. Estudos sobre esfirras integrais são escassos, e este estudo demonstrou a oportunidade da junção de ingredientes com alegação funcional, os quais apresentam tanto aplicabilidade na fabricação dos produtos quanto benefício aos consumidores. PALAVRAS-CHAVE: proteína de soja texturizada; intenção de compra; composição centesimal; valor calórico; testes sensoriais.