Growth performance of guppy fries was assessed by providing four different types of formulated diets comprised of some available ingredients (fish meal (FM), prawn meal (PM), meat and bone meal (MBM), wheat flour (WF), corn flour (CF), starch (S) and soya bean oil) and the result was compared with supplied commercial diet as control. After 30 days experimental period, diet 1 (FM: PM=6:1) showed the best result in case of length gain (0.48 cm) in guppies whereas lowest (0.01 cm) was reported from diet 2 (PM: MBM= 4:3). The body weight gain in guppies was also found higher (0.004 g) in diet 1 than other diets. Specific growth rate (SGR) of all formulated diets was between 1.03% to 4.8% while 0.7% was found in commercial diet. Maximum survival rate (95%) was recorded in formulated diet 1 whereas 80% was investigated in control .Growth trend of guppies from all the supplied formulated diets resulted better rather than the imported commercial diets. So, Commercial diets might be replaced by experimental diets for the better production of guppy.
The present study was carried out to evaluate the dietary effects of host‐associated lactic acid bacteria on growth performance, metabolic enzyme activities, and immune response of Macrobrachium rosenbergii juveniles. To formulate the test diets, a control (Con) diet was supplemented with a commercial probiotic and three host‐derived bacteria Enterococcus faecalis (EC), Lactococcus lactis I (LC‐I), and L. lactis II (LC‐II), which were previously isolated from the gastrointestinal tract of adult individuals of M. rosenbergii. Juvenile M. rosenbergii (0.65 ± 0.008 g) were randomly stocked at 20 individuals/100 L of fiberglass tanks with three replications for each test diet. After 50 days, juveniles fed the diets LC‐I and LC‐II showed significantly higher (p < .05) weight gain, specific growth rate, and the lowest feed conversion ratio. The analyses of glutamic oxaloacetate transaminase and glutamic pyruvate transaminase in muscle and hepatopancreas revealed significantly (p < .05) reduced values in LC‐I fed juveniles. The total hemocyte count and phenoloxidase activity were significantly increased (p < .05) in LC‐I and LC‐II fed juveniles. The relative mRNA expression patterns of immune‐related α2‐M, LGBP, ProPO, Cu, Zn‐SOD, TG, PE, AKP, and ACP genes were significantly (p < .05) upregulated in juveniles fed with LC‐I followed by the diet LC‐II. Finally, the study suggests that the growth performance and immune response of M. rosenbergii can be improved through supplementation of host‐associated L. lactis bacteria for its higher production.
Experimental infections of Aeromonas hydrophila in juvenile pangasius (Pangasianodon hypophthalmus) were studied. Five different challenge routes included intraperitoneal (IP) injection, intramuscular (IM) injection, oral administration, bath and agar implantation were used with different preparations of the bacteria to infect fish. The challenge experiments were continued for 15 days. A challenge dose of 4.6×106 colony forming unit (cfu) fish-1 was used for IP and IM injection and oral administration method. Generally, IP route was found more effective for infecting and reproducing clinical signs in fish that caused 100% mortality at the end of challenge. IM injection, oral and bath administration routes were also found effective for infecting and reproducing the clinical signs in fish to some extent. Agar implantation with fresh colonies of bacteria also caused 100% mortality of challenged fish very quickly with no visible clinical signs in fish. The major clinical signs of challenged fish included reddening around eyes and mouth, bilateral exophthalmia, hemorrhage and ulceration at fin bases and fin erosion.Progressive Agriculture 27 (3): 392-399, 2016
Data on growth, productivity, pigments and proximate composition of the four different indigenous marine microalgae (isolated from Cox's Bazar Coast) were collected to compare the growth performance, pigments and nutritional composition.
Chlorella
sp.,
Nannochloropsis
sp.,
Tetraselmis
sp. and
Chaetoceros
sp. are the four different marine microalgae. Growth curve was determined as the prerequisite to identify the stationary phase for each of the isolated microalgae. Data on growth curves were collected in terms of cell density and optical density to observe the growth rates and division per day. Isolated species were mass cultured in commercial culture medium. When the culture reached at stationary phase, microalgae were extracted to determine productivity, pigments, and proximate composition. The data of productivity (volumetric, areal and lipid productivity), pigments (Chlorophyll a, b, c, carotenoids, and phycobiliproteins), and proximate composition (protein, lipid, and carbohydrate) were significantly (p < 0.05) different among the four different microalgae. Therefore, this data will contribute to the selection of potential microalgae species through proper characterization for vast industrializations.
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