Abstract:Experimental infections of Aeromonas hydrophila in juvenile pangasius (Pangasianodon hypophthalmus) were studied. Five different challenge routes included intraperitoneal (IP) injection, intramuscular (IM) injection, oral administration, bath and agar implantation were used with different preparations of the bacteria to infect fish. The challenge experiments were continued for 15 days. A challenge dose of 4.6×106 colony forming unit (cfu) fish-1 was used for IP and IM injection and oral administration method. … Show more
“…The infected fish showed various clinical symptoms of MAS disease, namely ulcers, hemorrhage, pigmentation, swollen abdomen and eroded fins. Similar clinical signs have been found in common carp that suffer from MAS disease [6][7][8] . In addition, fish infected with 1.8×10 8 CPU/ml of A. hydrophila die between 8 and 24 h and show alterations in behavior, which are not observed in control fish 9,10 .…”
Background: Turmeric is known as a natural remedy to improve the immunity of organisms. This study aims to understand the effectiveness of turmeric-enriched pellets to improve the immunity of Clarias batrachus to Aeromonas hydrophila. Methods: The study was conducted from May to August 2020. C. batrachus fingerlings, 7-8 cm total length (TL) and 4-5 g (BW) at baseline, were kept in 30 L aquaria (10 fishes/aquarium; three replicated/treatment). Commercial pellets were mixed with turmeric powder. There were five treatment groups: P0 (control, no turmeric); P1 (0.5 g turmeric per Kg of pellets); P2 (0.7 g/Kg); P3 (0.9 g/Kg); Pp (positive control). Thirty days after being feed with turmeric-enriched pellets, all groups of fish were infected with 0.1 ml (108) of A. hydrophila suspension, intramuscularly. The P0 group did not receive injection, while Pp group were not fed with turmeric-enriched pellets but were infected with the bacteria. Fourteen days after infection, clinical signs and hematology of the fish were studied. Results: Pp fish showed heavy clinical signs of A. hydrophila, such as loss of balance, pigmentation, hemorrhages and ulcers. P0 fish did not show any symptoms, while the treated fish reveled some clinical signs of A. hydrophila to a lesser extent than Pp, indicating that the fish is able to face the A. hydrophila attack. Hematology for Pp fish revealed high white blood cells, indicating that the fish were infected. The blood condition of the P0 fish, as well as those of the turmeric-treated fish were normal. In general, the P3 fish showed the least clinical signs of A. hydrophila and normal blood condition, indicating that P3 treatment is best. Conclusion: The best turmeric dosage to improve the immunity of C. batrachus toward A. hydrophila infection is 0.9 g/Kg pellets.
“…The infected fish showed various clinical symptoms of MAS disease, namely ulcers, hemorrhage, pigmentation, swollen abdomen and eroded fins. Similar clinical signs have been found in common carp that suffer from MAS disease [6][7][8] . In addition, fish infected with 1.8×10 8 CPU/ml of A. hydrophila die between 8 and 24 h and show alterations in behavior, which are not observed in control fish 9,10 .…”
Background: Turmeric is known as a natural remedy to improve the immunity of organisms. This study aims to understand the effectiveness of turmeric-enriched pellets to improve the immunity of Clarias batrachus to Aeromonas hydrophila. Methods: The study was conducted from May to August 2020. C. batrachus fingerlings, 7-8 cm total length (TL) and 4-5 g (BW) at baseline, were kept in 30 L aquaria (10 fishes/aquarium; three replicated/treatment). Commercial pellets were mixed with turmeric powder. There were five treatment groups: P0 (control, no turmeric); P1 (0.5 g turmeric per Kg of pellets); P2 (0.7 g/Kg); P3 (0.9 g/Kg); Pp (positive control). Thirty days after being feed with turmeric-enriched pellets, all groups of fish were infected with 0.1 ml (108) of A. hydrophila suspension, intramuscularly. The P0 group did not receive injection, while Pp group were not fed with turmeric-enriched pellets but were infected with the bacteria. Fourteen days after infection, clinical signs and hematology of the fish were studied. Results: Pp fish showed heavy clinical signs of A. hydrophila, such as loss of balance, pigmentation, hemorrhages and ulcers. P0 fish did not show any symptoms, while the treated fish reveled some clinical signs of A. hydrophila to a lesser extent than Pp, indicating that the fish is able to face the A. hydrophila attack. Hematology for Pp fish revealed high white blood cells, indicating that the fish were infected. The blood condition of the P0 fish, as well as those of the turmeric-treated fish were normal. In general, the P3 fish showed the least clinical signs of A. hydrophila and normal blood condition, indicating that P3 treatment is best. Conclusion: The best turmeric dosage to improve the immunity of C. batrachus toward A. hydrophila infection is 0.9 g/Kg pellets.
“…However, the challenge test via intramuscular injection is an unusual administrative way for Aeromonas sp. infection; instead, the pathogen showed the best clinical signs after administrating via IP injection (Sarker & Faruk, 2016); hence, the challenge test with Aeromonas sp. via IM injection requires careful observation to elucidate studies further.…”
The study focused on identifying autochthonous bacteria from the indigenous catfish intestine and evaluating their impacts on survival, growth and against Aeromonas veronii infection in Clarias batrachus and Heteropneustes fossilis under laboratory conditions. Candidate bacteria were isolated using Nutrient Agar (NA) and de Man, Rogosa and Sharpe (MRS) agar in duplicate. An immersion technique was employed to determine the biosafety of these isolates in both catfish species at a dose of 107 CFU/mL. Three experimental diets: D1 (basal diet + putative Bacillus sp., 108–109 CFU/g), D2 (basal diet + 3% BioFav Aqua) and D3 (basal diet + putative Lactobacillus sp., 108–109 CFU/g) were prepared to conduct a 4‐week long feeding trial, whereas the control diet received no probiotic supplementation. The probiotic performance was analysed by comparing the growth performance and survival of C. batrachus and H. fossilis. Resistance against Aeromonas veronii infection was determined by a challenge test of catfish. Initially, 16 autochthonous isolates were collected, and among them, an isolate from C. batrachus intestine and another from the gut of H. fossilis were selected for further studies. A series of biochemical analyses identified that the putative bacteria were Bacillus sp. and Lactobacillus sp., respectively. The biosafety test showed no clinical signs of infection or mortality among experimental catfishes. All probiotic‐treated fish showed higher growth increments than control. Incorporation of Bacillus sp. and Lactobacillus sp. in the basal diet significantly (p < 0.05) improved growth and survival in both catfish, where Bacillus sp. showed the best effects in C. batrachus, and Lactobacillus sp. in H. fossilis. Intramuscular (IM) injection with A. veronii revealed that probiotic‐treated fish had better survival rates. The study demonstrated the potentiality of autochthonous bacteria to enhance growth, survival, feed efficiency and immunity and hence recommended as a better health management approach in catfish aquaculture.
“…On the other hand, infection by immersion did not reproduce the disease. Notably, the difficulty to reliably induce disease through routes of entry beside injection is well documented in multiple fish species, including cyprinids, for motile Aeromonas, in particular for A. hydrophila, which is the most studied of the motile Aeromonas (Das Mahapatra et al 2008, Sarker & Faruk 2016, Zhang et al 2016, Peatman et al 2018.…”
In northern Vietnam, a disease called ‘red spot disease’ has been causing high morbidity and mortality in populations of farmed grass carp Ctenopharyngodon idella for about 2 decades. The name ‘red spot disease’ refers to a condition characterised by haemorrhagic lesions, reddening and ulceration of the skin. Eight different bacterial isolates, namely Aeromonas hydrophila, A. sobria, Staphylococcus epidermidis, Vibrio alginolyticus, Pseudomonas fluorescens, P. luteola, Citrobacter freundii and P. putida, were isolated from diseased grass carp and used for experimental infection of the same species. Fish were challenged with the different bacterial isolates both by immersion and intramuscular injection. Different concentrations of bacteria were tested to evaluate their pathogenicity. Injection with 1 × 105 CFU of A. hydrophila and A. sobria resulted in clinical signs identical to those of red spot-diseased grass carp in Vietnam. None of the other bacterial isolates tested caused any morbidity or mortality in fish challenged either intramuscularly (1 × 106 CFU) or by bath immersion (1 × 106 or 1 × 108 CFU ml-1).
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