Abstract:Data on growth, productivity, pigments and proximate composition of the four different indigenous marine microalgae (isolated from Cox's Bazar Coast) were collected to compare the growth performance, pigments and nutritional composition.
Chlorella
sp.,
Nannochloropsis
sp.,
Tetraselmis
sp. and
Chaetoceros
sp. are the four different marine microalgae. Growth curve was determined as the prerequisite to identify the station… Show more
“…Nannochloropsis sp. cultured in Conway media showed similar growth phases [26]. The cell densities were changed significantly (p<0.05) at exponential and stationary phases.…”
Nitrogen is one of the key growth factors and structural constituent of the cellular molecules of Nannochloropsis sp. This study aimed to determine the influence of nitrogen stress in the form of potassium nitrate (KNO3 ) by altering the standard recommended nitrogen concentration to characterize the growth performance, proximate composition, and pigments content. The nitrogen concentration was arranged in following order for the culture of Nannochloropsis sp.: 50 g/L, 75 g/L, 100 g/L (standard), 125 g/L, and 150 g/L of KNO3 . All growth parameters, proximate composition, and pigment contents showed significant (p < 0.05) changes among the treatments. Cell density was increased by 2.48 folds, SGR was increased by 56.9%, and cells were doubled in number at a pace of 12.192 hours faster at the maximum nitrogen concentration (150g/L KNO3 ). The maximum protein content was 54 ± 1.05% at 150 g/L KNO3 while the highest lipid content was 36 ± 0.32% at 75 g/L KNO3 . The highest carbohydrate content was 24 ± 2.51% at 100 g/L KNO3 . The chlorophyll a content was increased to 0.632 ± 0.049 µg/L at 150 g/L KNO3 , while the highest carotenoids content was 3.301 ± 0.052 µg/ml at the 75g/L KNO3 . On the other hand, phycobiliprotein content was the highest 0.028 mg/g for the standard concentration of KNO3 . This study provides the commercial cultivation strategy for Nannochloropsis sp. by revealing the biomass productivity, biochemical and bioactive compounds status at different nitrogen-based culture conditions that will ensure their multipurpose use in aquaculture, pharmaceuticals, and nutraceuticals sectors.
“…Nannochloropsis sp. cultured in Conway media showed similar growth phases [26]. The cell densities were changed significantly (p<0.05) at exponential and stationary phases.…”
Nitrogen is one of the key growth factors and structural constituent of the cellular molecules of Nannochloropsis sp. This study aimed to determine the influence of nitrogen stress in the form of potassium nitrate (KNO3 ) by altering the standard recommended nitrogen concentration to characterize the growth performance, proximate composition, and pigments content. The nitrogen concentration was arranged in following order for the culture of Nannochloropsis sp.: 50 g/L, 75 g/L, 100 g/L (standard), 125 g/L, and 150 g/L of KNO3 . All growth parameters, proximate composition, and pigment contents showed significant (p < 0.05) changes among the treatments. Cell density was increased by 2.48 folds, SGR was increased by 56.9%, and cells were doubled in number at a pace of 12.192 hours faster at the maximum nitrogen concentration (150g/L KNO3 ). The maximum protein content was 54 ± 1.05% at 150 g/L KNO3 while the highest lipid content was 36 ± 0.32% at 75 g/L KNO3 . The highest carbohydrate content was 24 ± 2.51% at 100 g/L KNO3 . The chlorophyll a content was increased to 0.632 ± 0.049 µg/L at 150 g/L KNO3 , while the highest carotenoids content was 3.301 ± 0.052 µg/ml at the 75g/L KNO3 . On the other hand, phycobiliprotein content was the highest 0.028 mg/g for the standard concentration of KNO3 . This study provides the commercial cultivation strategy for Nannochloropsis sp. by revealing the biomass productivity, biochemical and bioactive compounds status at different nitrogen-based culture conditions that will ensure their multipurpose use in aquaculture, pharmaceuticals, and nutraceuticals sectors.
“…Then, the growth media was prepared with required nutrients; salinity and pH were adjusted to 26 ppt and 7.8, optimum for the culture of Nannochloropsis sp. [3] . Pure strains from the stock were transferred to 20 L glass tanks for mass culture at room temperature with a 12 h light: 12 h dark photoperiod, commencing with a volume of 5 L and subsequently scaling up to 16 L. Every two days, media was added to the culture tank until it reached the desired volume.…”
Section: Experimental Design Materials and Methodsmentioning
confidence: 99%
“…A 16-day culture trial was performed to determine the native strain's stationary phase in a previous investigation [3] and according to this findings microalgae was cultured, harvested by centrifugation [4] and dried in a hot air oven at 60 °C temperature for overnight [5] . The dried biomass was pulverized using small sized mortar and pestle.…”
Section: Experimental Design Materials and Methodsmentioning
Microalgae can form renewable feedstock for many sectors. However, most microalgae commercially cultivated are difficult to grow in colder climates. To overcome this problem one could cultivate cold tolerant algae. One such alga is the red marine alga Porphyridium purpureum. It is currently often cultivated in bag systems in limited volumes. In this study we cultivated P. purpureum at pilot-scale in horizontal tubular reactors in a greenhouse. P. purpureum was grown in batch mode in a total volume of either 350, 500, 1000 or 1500 L. A total of 28 batches, spread over two years, were cultivated reaching a maximum dry weight of 1.94 g L−1. The growth was continuously monitored to calculate the productivities and growth rates. The P and N concentrations were measured frequently during four batches to calculate the P and N usage per gram biomass. To calculate CO2 fixation efficiencies the total CO2 consumption was monitored during 6 of the 28 batches. A maximum productivity and growth rate of respectively 0.195 g L−1 day−1 and 0.233 day−1 were achieved while the P and N usage varied from 3.2–6.19 and 14.5–36.6 mg g−1 biomass for P and N, respectively. Growth was supported between 10 °C and 30 °C, while temperatures above 34—35 °C proved to be detrimental. The CO2 efficiencies differed among the 6 tested periods with the highest utilization efficiency being 13.5% and the lowest a mere 1.5%. Further large-scale, continuous, tests would be needed to fully optimize the cultivation of P. purpureum in larger volumes.
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