This study aims to determine the content and total levels of secondary metabolites of ethanol extract of moringa seed (Moringa oleifera Lam.). Extraction of moringa seed using the maceration method to obtain a filtrate. The filtrate obtained was concentrated using a rotary evaporator to obtain a concentrated extract. The extract was tested qualitatively for alkaloids, flavonoids, saponins, and tannins using a suitable reagent with the test parameters. The quantitative test was using UV-Visible spectrophotometry. Alkaloids using test parameters equivalent total alkaloid quinine, flavonoids use parameter test equivalent of the total flavonoids quercetin, saponin using test parameters from Quillaja total saponins and tannins quantitative bark using test parameters total tannin tannic acid equivalent. The result showed that moringa seed positive for alkaloids characterized by orange deposition, flavonoids are characterized by the formation of the yellow color orange, saponin their stable foam, and tannins are marked in black. Quantitative test results alkaloids of 916,87 µg/g, flavonoids of 0.255%, saponin of 6.367%, and tannin of 3,724.5 µg/g. Keywords: Moringa oleifera Lam., secondary metabolites, spectrophotometry
The objective of this study is to determine the effect and the effective dose of ethanolic extract of Red Gendola (Basella alba L.) leaves on creatinine, urea and description of histological tubular kidney of diabetic male rats induced by streptozotocin. 30 male rats devided into six groups : groups I, II, and III were the control groups and groups IV, V, and VI were the experimental groups. Groups II, III, IV, V, and VI are given induction of STZ 40 mg/kg BW in a single dose i.p on day 0. Normal control group I and negatif control group II were given only Sodium-CMC 0,5%; positive control group III was given glibenclamide 0,45 mg/kg BW; group IV, V, and VI were given each extract at a dose of 100, 200, and 400 mg/kg BW in p.o every day. The obtained data were analyzed using one way Anova test at the level of 95 % early followed by LSD Post Hoc Test, and the level of regeneration of histological tubular kidney was observed by HE staining using Olympus BX-51 microscope with a scale of 100 µm and scoring data result of kidney tubular damage levels were analyzed by Kruskal-Wallis test followed by Mann-Whitney test to see the differences between treatments. The results showed that ethanolic extract of red gendola with dose of 100 mg / kg BW give significant effect to decrease the creatinine content and 200 mg / kg BW give effect of decrease of ureum level of diabetic white male rats. Moreover, red gendola ethanolic extract has also an effect on cell regeneration in renal tubules with an effective dose of 400 mg / kg BW on diabetic male rats.
Nephroprotective compound is a compound that has the ability to protect the kidneys from Keywords: Nefroprotective, ethylene glycol, Abelmoschus manihot, Orthosiphon stamineus ABSTRAKSenyawa yang bersifat nefroprotektif adalah senyawa yang memiliki kemampuan melindungi ginjal dari gangguan ginjal yang disebabkan oleh radikal bebas. Penelitian ini bertujuan untuk mengetahui efek nefroprotektif kombinasi ekstrak daun gedi merah dan daun kumis kucing serta mengetahui kombinasi dosis ekstrak yang efektif sebagai nefroprotektif. Penelitian ini menggunakan Rancangan Acak Kelompok dengan 6 kelompok perlakuan terdiri dari 5 ekor tikus. Kelompok I kontrol normal. Kelompok II kontrol negatif. Semua kelompok ekstrak diberikan etilen glikol 0,75%, amonium klorida 2% & perlakuan ekstrak untuk masing-masing kelompok. Kelompok III (kombinasi 1) diberikan ekstrak daun gedi merah 100 mg/kg BB dan daun kumis kucing 200 mg/kg BB. Kelompok IV (kombinasi 2) diberikan ekstrak daun gedi merah 50 mg/kg BB dan daun kumis kucing 100 mg/kg BB. Kelompok V diberikan ekstrak daun gedi merah 100 mg/kg BB. Kelompok VI ekstrak daun kumis kucing 200 mg/kg BB. Perlakuan diberikan selama 14 hari dan dilakukan pengukuran kadar kreatinin dan ureum pada
This study aims to determine the content of secondary metabolites and the levels of total secondary metabolites in ethanol extract of Okra (Abelmoschus esculentus L. Moench) fruit with UV-Vis Spectrophotometry method. Okra (Abelmoschus esculentus L. Moench) fruit extract was prepared by maceration method with 96% ethanol. The extract was concentrated using a rotary evaporator that followed by a qualitative test for alkaloids, flavonoids, saponins, and tannins using suitable reagents with the test parameters, while for the qualitative assay using a UV-Vis Spectrophotometry. Qualitative analysis of alkaloids using test parameters of total alkaloids equivalent to quinine, flavonoid using parameter of total flavonoids equivalent to quercetin, saponin using parameters of standard saponins total from the Quillaja Bark, and tannin using test parameters of total tannins equivalent to tannic acid. Qualitative test results indicated that the ethanol extract of Okra fruit tested positive for alkaloids content wich characterized by the orange deposition, flavonoids characterized by the formation of an orange-yellow color, a stable foam for saponins and tannins marked in black. The quantitative analysis resulted alkaloids of 2228.06 mg/gram, flavonoids of 2.79 mg/gram, saponins of 10.03 mg/gram, and tannins of 1973.27 mg/gram. Keywords: Okra fruit, secondary metabolites, UV- Vis spectrophotometry, qualitative, quantitative.
UJI EFEK KOMBINASI EEDS DAN DPW TERHADAP PENURUNAN KADAR KOLESTEROLTOTAL DAN GLUKOSA DARAH TIKUSPUTIH JANTAN HIPERKOLESTEROLEMIA-DIABETES Joni Tandi1), Alpina Na’i1), Aprince Basilingan1)1)Program Studi S1 Farmasi, STIFA Pelita Mas PaluEmail :jonitandi757@yahoo.com ABSTRACT The study aimed to investigate the effect of combination on EEDS soursop extract ethanol and ethanol extract of EEDPW fragrant pandan leaves, lowering total cholesterol and blood glucose in male white rats induced high cholesterol and streptozotocin and did not cause toxicity in mice and determine the dose of EEDS and EEDPW leaf which effectively decreases total cholesterol and blood glucose levels in male white rats. This research is a laboratory experiment using posttest randomized controlled group design with posttest randomized control design with 8 treatment groups consisting of 5 test animals. Group 1 (normal control). group 2 (negative control) was given a NaCC Na suspension of 0.5% w / v. Group 3 positive (simvastatin suspension) and (metformin suspension). Group 4 was given EEDS 150 mg / kg BW. Group 5 was given EEDPW 600 mg / kg BW. Group 6 was given a combination of (I) EEDS, EEDPW 150 + 600 mg / kg BW. Group 7 was given a combination of (II) EEDS, EEDPW 75 + 300 mg / kg BW. The results showed that; there is a secondary metabolite compound in soursop leaf extract and pandan wangi, EEDS combination, EEDPW can decrease total cholesterol and blood glucose level, the most effective dose in decreasing the concentration of soursop leaves leaf sole 150 mg / kg BW. While the most effective dose is a combination (II) EEDS and EEDPW with a dose of 75 + 300 mg / kg BW, giving the maximum effect on decreased blood glucose levels. Keywords:.High Cholesterol Feed, Streptozotocin, Annona muricata L, Pandanus amarylifollius Roxb, Total Cholesterol ABSTRAK Penelitian bertujuan untuk mengetahui efekkombinasi pada ekstraketanol daun sirsak EEDS dan ekstrak etanol daun pandan wangi EEDPW, penurunkan kadar kolesterol total danglukosa darah tikus putih jantan yang diinduksi pakan tinggi kolesterol dan streptozotocin dan tidak menimbulkan toksisitas pada tikus dan menentukan dosis berapa EEDS dan daun EEDPW yang efektif menurunkan kadar kolesterol total dan glukosa darah pada tikus putih jantan. Penelitian ini merupakan eksperimen laboratorium dengan menggunakan rancangan penelitian modifikasi posttest randomized controlled group design dengan 8 kelompok perlakuan yang terdiri dari 5 ekor hewan uji. Kelompok 1(kontrol normal) dan kelompok 2(kontrol negatif) diberi suspensi Na CMC 0,5% b/v. Kelompok 3 kontrol positif (suspensi simvastatin) dan (suspensi metformin). Kelompok 4 diberiEEDS 150 mg/kg BB. Kelompok 5 diberiEEDPW 600 mg/kg BB. Kelompok 6 diberi kombinasi (I) EEDS, EEDPW 150+600 mg/kg BB. Kelompok 7 diberi kombinasi (II) EEDS, EEDPW 75+300 mg/kg BB. Hasil penelitian menunjukkan bahwa;terdapat senyawa metabolit sekunder pada ekstrak daun sirsak dan pandan wangi,pemberian kombinasi EEDS, EEDPWdapat menurunkan kadar kolesterol total dan kadar gluosa darah, dosis yang paling efektif dalam penurunan kadar koleserol daun sirsak dosis tunggal 150 mg/kg BB.Sedangkan dosis paling efektif adalah kombinasi (II) EEDS dan EEDPW dengan dosis 75+300 mg/kg BB, memberikan efek maksimal terhadap penurunan kadar glukosa darah. Kata Kunci : Pakan Tinggi Kolesterol, Streptozotocin, Annona muricata L,Pandanus amarylifollius Roxb, Kolesterol Total
Hiperkolesterolemia adalah peningkatan kadar kolesterol di dalam darah melebihi batas yang diperlukan oleh tubuh. Penelitan ini bertujuan menguji kandungan senyawa fitokimia ekstrak etanol biji labu kuning, efek pemberian ekstrak etanol biji labu kuning dan perbedaan efek ekstrak dengan dosis bertingkat terhadap penurunan degenerasi sel beta pankreas tikus putih jantan hiperkolesterolemia diabetes. Penelitian eksperimen laboratorium ini menggunakan hewan uji sebanyak 30 ekor tikus dibagi dalam enam kelompok perlakuan. Kelompok 1 (kontrol normal) diberikan Na-CMC 0,5% dan kelompok 2 (kontrol negatif) diberi pakan tinggi kolesterol, suspensi Streptozotocin 35 mg/kgBB dan Na-CMC 0,5% b/v; kelompok 3 diberi metformin 9 mg/kgBB per oral, pakan tinggi kolesterol dan suspensi streptozotocin 35 mg/kgBB; kelompok 4, 5 dan 6 masing-masing diberikan dosis 270, 360, dan 450 mg/kgBB per oral, pakan tinggi kolesterol dan suspensi streptozotocin 35 mg/kgBB. Gambaran tingkat kerusakan histopatologi pankreas diamati dengan pewarnaan HE menggunakan mikroskop Olympus BX-51 perbesaran 200x. Hasil penelitian menunjukkan: Terdapat senyawa alkaloid, flavonoid, polifenol, saponin,dan tannin pada ekstrak etanol biji labu kuning; ekstrak etanol biji labu kuning dosis 360 dan 450 mg/kgBB efektif menurunkan degenerasi jaringan pankreas tikus hiperkolesterolemia diabetes dan pemberian ekstrak etanol biji labu kuning dosis 270 mg/kgBB tidak memberikan efek maksimal jika dibandingkan dengan dosis 360 mg/kgBB dan dosis 450 mg/kgBB terhadap regenerasi sel organ pankreas tikus putih jantan hiperkolesterolemia diabetes. Hypercholesterolemia is an abnormal increase in blood cholesterol levels. This study aimed to identify the phytochemical content of pumpkin seed ethanol extract, the effect of pumpkin seed ethanol extract and the difference in the effect of various doses of extract in decreasing pancreatic beta cell degeneration in male diabetic hypercholesterolemia rat. The experimental research in laboratory used 30 rats as test animals, divided into six group. Group 1 (normal control administered with 0.5% Na CMC and group 2 (negative control) administered with high cholesterol diet, Streptozotocin 35 mg/kgBWand 0.5% Na-CMC b/v; Group 3 orally administered with metformin 9 mg/kgBW, high cholesterol diet, streptozotocin 35 mg/kgBW; group 4,5 and 6 orally administered with extract at doses of 270, 360, dan 450 mg/kgBB, high cholesterol diet, streptozotocin 35 mg/kgBW. Histopathology examination to determine pancreas damage was observed by HE staining using microscop Olympus BX-51 200x. The results showed the presence of alkaloids, flavonoids, poliphenol, saponins,andtanninsin phumpkin seeds ethanol extract; Ethanol extract of phumpkin seeds at doses of 360 dan 450 mg/kgBW effectively decreased degeneration of pancreatic tissue of diabetic hypercholesterolemia rat and ethanol extract of phumpkin seeds at the dose of 270 mg/kgBW did not show maximum effect as compared to the doses of 360 and 450 mg/kg BE on cell regeneration of pancreatic tissue of diabetic hypercholesterolemia rat
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