Efek Ekstrak Etanol Daun Gendola Merah (Basella alba L.) terhadap Kadar Kreatinin, Ureum dan Deskripsi Histologis Tubulus Ginjal Tikus Putih Jantan (Rattus norvegicus) Diabetes yang Diinduksi Streptozotocin

Abstract

The objective of this study is to determine the effect and the effective dose of ethanolic extract of Red Gendola (Basella alba L.) leaves on creatinine, urea and description of histological tubular kidney of diabetic male rats induced by streptozotocin. 30 male rats devided into six groups : groups I, II, and III were the control groups and groups IV, V, and VI were the experimental groups. Groups II, III, IV, V, and VI are given induction of STZ 40 mg/kg BW in a single dose i.p on day 0. Normal control group I and negatif control group II were given only Sodium-CMC 0,5%; positive control group III was given glibenclamide 0,45 mg/kg BW; group IV, V, and VI were given each extract at a dose of 100, 200, and 400 mg/kg BW in p.o every day. The obtained data were analyzed using one way Anova test at the level of 95 % early followed by LSD Post Hoc Test, and the level of regeneration of histological tubular kidney was observed by HE staining using Olympus BX-51 microscope with a scale of 100 µm and scoring data result of kidney tubular damage levels were analyzed by Kruskal-Wallis test followed by Mann-Whitney test to see the differences between treatments. The results showed that ethanolic extract of red gendola with dose of 100 mg / kg BW give significant effect to decrease the creatinine content and 200 mg / kg BW give effect of decrease of ureum level of diabetic white male rats. Moreover, red gendola ethanolic extract has also an effect on cell regeneration in renal tubules with an effective dose of 400 mg / kg BW on diabetic male rats.

Histopathology of Pre-diabetic White Rat (Rattus norvegicus L.) Renal After Treatment with Turmeric Powder and Organic Quail Eggs

“…The data from the study were tested for homogeneity and normality using the Lavene test and then analysed using ANOVA that is based on Completely Randomized Design (CRD) on a 95% (α = 0.05) confidence level and continued with Duncan test (Isdadiyanto, 2018). Total analysis was calculated using SPSS 24 software (Tandi et al, 2017).…”

POTENSI EKSTRAK ETIL ASETAT Coprinus comatus TERHADAP KADAR UREUM DAN KREATININ PADA TIKUS PUTIH MODEL DIABETES

“…Ketidak seimbangan antara radikal bebas dengan antioksidan endogen (stress oksidatif) memicu terjadinya peroksidasi lipid pada membran sel endotel glomerulus, sehingga laju filtrasi glomerulus terganggu. Laju filtrasi glomerulus yang terganggu akan meningkatkan kadar ureum dan kreatinin sehingga kadar ureum dan kreatinin dapat menjadi parameter untuk menilai fungsi ginjal normal (Tandi et al, 2017).…”

Uji Nefroterapi Ekstrak Etanol Daun Afrika (Vernonia amygdalina Delile) dengan Parameter Ureum Tikus Putih Jantan yang Diinduksi Gentamisin

“…Akibatnya ginjal menjadi organ sasaran utama dari efek toksik. 2 Ginjal membuang banyak toksin dan zat asing lainnya yang diproduksi oleh tubuh atau pencernaan, seperti pestisida, makanan, dan obat-obatan. 3 Salah satu obat yang dapat merusak ginjal yaitu antibiotik golongan aminoglikosida, seperti misalnya gentamisin.…”

“…Ureum merupakan hasil utama metabolisme protein dalam tubuh. 2 Jika terjadi gangguan, zat ini akan meningkat jumlahnya di dalam darah dan menjadi indikasi rusaknya fungsi ginjal. 7 Penyakit ginjal yang tidak segera diobati dan ditangani maka kemungkinan akan terjadi gagal ginjal.…”