Scutellaria baicalensis Georgi is a traditional Chinese herbal medicine mainly containing flavonoids that contribute to its bioactivities. In this study, the distributions and dynamic changes of flavonoid levels in various organs of S. baicalensis at different development stages were investigated by UHPLC-QTOF-MS/MS and HPLC-DAD methods. The results indicated that the metabolic profiles of S. baicalensis changed with growth and development. During the initial germination stage, the seeds mainly contained flavonols. With growth, the main kinds of flavonoids in S. baicalensis changed from flavonols to flavanones and flavones. The results also revealed that the accumulation of flavonoids in S. baicalensis is organ-specific. The flavones without 4′-OH groups mainly accumulate in the root and the flavanones mainly accumulate in aerial organs. Dynamic accumulation analysis showed that the main flavonoids in the root of S. baicalensis accumulated rapidly before the full-bloom stage, then changed to a small extent. The results suggested the proper harvest time for the aerial parts was at the initial stage of reproductive growth and the flower buds should be collected before flowering. This study deepening the knowledge of S. baicalensis should provide valuable information for guiding the scientific cultivation of this plant and the development and utilization of S. baicalensis.
Two new transition metal(II) complexes [M(hdpa) 2 -(N(CN) 2 ) 2 ] (M ϭ Mn (1), Co (2); hdpa ϭ 2,2'-dipyridylamine) have been prepared and characterized structurally and magnetically. Both compounds crystallize in the monoclinic space group C2/c. 1 and 2 are isotypic with the unit cell parameters a ϭ 8.634(9), b ϭ 13.541 (14), c ϭ 21.99(2) Å , β ϭ 94.806(18)°, and V ϭ 2562(5) Å 3 for 1, a ϭ 8.617(3) Å , b ϭ 13.629(5)Å , c ϭ 21.598(8)Å , β ϭ 94.593(6)°, and V ϭ 2528.4(15)Å 3 for 2, and Z ϭ 4 for both. According to X-ray crystallographic studies, each metal(II) ion was
Cancer
cells rely on fatty acid synthase (FASN), a key enzyme for
de novo biosynthesis of long chain fatty acids, to sustain their proliferative
potential and drive invasion. Unfortunately, conventional FASN assays
are technically inadequate for discerning otherwise elusive FASN activity
in complex biological milieux, which has hindered progress in the
functional study of FASN and development of its inhibitors. Here,
we describe a chemical probe with unprecedented selectivity and sensitivity
for the labeling of active FASN in living cells, thus demonstrating
a new analytical modality for visualizing endogenous FASN activity
and exploring opportunities for drug discovery.
The complex [Cu 2 (L) 2 (µ 2 -C 2 O 4 )]·CH 3 OH (1) has been synthesized and characterized by IR, UV, ESR and variable temperature magnetic susceptibility measurement, where L ϭ 1-(ethylamino)-2-(salicylideneamino)ethane. The crystal X-ray diffraction reveals that complex 1 has a µ 2 -C 2 O 4 2Ϫ bridge. The complex exhibits ferromagnetic couplings between the copper atoms bridged
Synthese, Kristallstruktur und Eigenschaften eines zweikernigen Kupfer(II)-Komplexes mit einer OxalatbrückeInhaltsübersicht. Der Komplex [Cu 2 (L) 2 (µ 2 -C 2 O 4 )]·CH 3 OH(1)(L ϭ 1-(ethylamino)-2-(salicylidenamino)ethan) wurde synthetisiert und durch IR, UV, ESR sowie durch magnetische Suszeptibilitäts-Messungen bei variabler Temperatur charakterisiert. Die Röntgen-Kri-
Cobalamin-independent methionine synthases (MS) are zinc-binding methyltransferases that catalyze de novo methionine biosynthesis in higher plants, which are enzymes critically involved in seed germination and plant growth. Here, we report a highly selective sulfonyl fluoride-based probe for chemoproteomic profiling of MS enzymes in living systems of the model plant Arabidopsis thaliana, as implemented in in-gel-, mass spectrometry-, and imaging-based platforms. This probe holds promise for facilitating and accelerating fundamental research and industrial application of MS enzymes, particularly in the contexts of MS1/2targeting herbicide screening and design.
We report a quantitative chemoproteomic approach that utilizes a clickable photoreactive probe for global profiling of celastrol targets, which may significantly improve the current understanding of celastrol’s mode of action.
Sulfuric acid solution containing ferric iron is the extractant for industrial heap bioleaching of copper sulfides. To start a heap bioleaching plant, sulfuric acid is usually added to the irrigation solution to maintain adequate acidity (pH 1.0–2.0) for copper dissolution. An industrial practice of heap bioleaching of secondary copper sulfide ore that began with only water irrigation without the addition of sulfuric acid was successfully implemented and introduced in this manuscript. The mineral composition and their behavior related to the production and consumption of sulfuric acid during the bioleaching in heaps was analyzed. This indicated the possibility of self-generating of sulfuric acid in heaps without exogenous addition. After proving by batches of laboratory tests, industrial measures were implemented to promote the sulfide mineral oxidation in heaps throughout the acidifying stages, from a pH of 7.0 to 1.0, thus sulfuric acid and iron was produced especially by pyrite oxidation. After acidifying of the heaps, adapted microbial consortium was inoculated and established in a leaching system. The launch of the bioleaching heap and finally the production expansion were realized without the addition of sulfuric acid, showing great efficiency under low operation costs.
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